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The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals

OBJECTIVE: The present study evaluated the use of a reagent to stabilize the DNA extracted from human dental tissues stored under different temperature conditions and time intervals. MATERIAL AND METHODS: A total of 161 teeth were divided into two distinct groups: intact teeth and isolated dental pu...

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Autores principales: TERADA, Andrea Sayuri Silveira Dias, da SILVA, Luiz Antonio Ferreira, GALO, Rodrigo, de AZEVEDO, Aline, GERLACH, Raquel Fernanda, da SILVA, Ricardo Henrique Alves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade de Odontologia de Bauru da Universidade de São Paulo 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126830/
https://www.ncbi.nlm.nih.gov/pubmed/25141206
http://dx.doi.org/10.1590/1678-775720130683
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author TERADA, Andrea Sayuri Silveira Dias
da SILVA, Luiz Antonio Ferreira
GALO, Rodrigo
de AZEVEDO, Aline
GERLACH, Raquel Fernanda
da SILVA, Ricardo Henrique Alves
author_facet TERADA, Andrea Sayuri Silveira Dias
da SILVA, Luiz Antonio Ferreira
GALO, Rodrigo
de AZEVEDO, Aline
GERLACH, Raquel Fernanda
da SILVA, Ricardo Henrique Alves
author_sort TERADA, Andrea Sayuri Silveira Dias
collection PubMed
description OBJECTIVE: The present study evaluated the use of a reagent to stabilize the DNA extracted from human dental tissues stored under different temperature conditions and time intervals. MATERIAL AND METHODS: A total of 161 teeth were divided into two distinct groups: intact teeth and isolated dental pulp tissue. The samples were stored with or without the product at different time intervals and temperature. After storage, DNA extraction and genomic DNA quantification were performed using real-time PCR; the fragments of the 32 samples that represented each possible condition were analyzed to find the four pre-selected markers in STR analysis. RESULTS: The results of the quantification showed values ranging from 0.01 to 10,246.88 ng/μL of DNA. The statistical difference in the quantity of DNA was observed when the factors related to the time and temperature of storage were analyzed. In relation to the use of the specific reagent, its use was relevant in the group of intact teeth when they were at room temperature for 30 and 180 days. The analysis of the fragments in the 32 selected samples was possible irrespective of the amount of DNA, confirming that the STR analysis using an automated method yields good results. CONCLUSIONS: The use of a specific reagent showed a significant difference in stabilizing DNA in samples of intact human teeth stored at room temperature for 30 and 180 days, while the results showed no justification for using the product under the other conditions tested.
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spelling pubmed-41268302014-08-11 The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals TERADA, Andrea Sayuri Silveira Dias da SILVA, Luiz Antonio Ferreira GALO, Rodrigo de AZEVEDO, Aline GERLACH, Raquel Fernanda da SILVA, Ricardo Henrique Alves J Appl Oral Sci Original Articles OBJECTIVE: The present study evaluated the use of a reagent to stabilize the DNA extracted from human dental tissues stored under different temperature conditions and time intervals. MATERIAL AND METHODS: A total of 161 teeth were divided into two distinct groups: intact teeth and isolated dental pulp tissue. The samples were stored with or without the product at different time intervals and temperature. After storage, DNA extraction and genomic DNA quantification were performed using real-time PCR; the fragments of the 32 samples that represented each possible condition were analyzed to find the four pre-selected markers in STR analysis. RESULTS: The results of the quantification showed values ranging from 0.01 to 10,246.88 ng/μL of DNA. The statistical difference in the quantity of DNA was observed when the factors related to the time and temperature of storage were analyzed. In relation to the use of the specific reagent, its use was relevant in the group of intact teeth when they were at room temperature for 30 and 180 days. The analysis of the fragments in the 32 selected samples was possible irrespective of the amount of DNA, confirming that the STR analysis using an automated method yields good results. CONCLUSIONS: The use of a specific reagent showed a significant difference in stabilizing DNA in samples of intact human teeth stored at room temperature for 30 and 180 days, while the results showed no justification for using the product under the other conditions tested. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2014 /pmc/articles/PMC4126830/ /pubmed/25141206 http://dx.doi.org/10.1590/1678-775720130683 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
TERADA, Andrea Sayuri Silveira Dias
da SILVA, Luiz Antonio Ferreira
GALO, Rodrigo
de AZEVEDO, Aline
GERLACH, Raquel Fernanda
da SILVA, Ricardo Henrique Alves
The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals
title The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals
title_full The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals
title_fullStr The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals
title_full_unstemmed The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals
title_short The use of a DNA stabilizer in human dental tissues stored under different temperature conditions and time intervals
title_sort use of a dna stabilizer in human dental tissues stored under different temperature conditions and time intervals
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4126830/
https://www.ncbi.nlm.nih.gov/pubmed/25141206
http://dx.doi.org/10.1590/1678-775720130683
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