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Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study

INTRODUCTION: Lymph nodes are one of the main sites where an effective immune response develops. Normally, axillary nodes are the first place where breast cancer produces metastases. Several studies have demonstrated the importance of immune cells, especially dendritic cells, in the evolution of bre...

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Autores principales: López, Carlos, Callau, Cristina, Bosch, Ramon, Korzynska, Anna, Jaén, Joaquín, García-Rojo, Marcial, Bueno, Gloria, Salvadó, Mª Teresa, Álvaro, Tomás, Oños, Montse, Fernández-Carrobles, María del Milagro, Llobera, Montserrat, Baucells, Jordi, Orero, Guifré, Lejeune, Marylène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4127922/
https://www.ncbi.nlm.nih.gov/pubmed/25091015
http://dx.doi.org/10.1136/bmjopen-2014-005643
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author López, Carlos
Callau, Cristina
Bosch, Ramon
Korzynska, Anna
Jaén, Joaquín
García-Rojo, Marcial
Bueno, Gloria
Salvadó, Mª Teresa
Álvaro, Tomás
Oños, Montse
Fernández-Carrobles, María del Milagro
Llobera, Montserrat
Baucells, Jordi
Orero, Guifré
Lejeune, Marylène
author_facet López, Carlos
Callau, Cristina
Bosch, Ramon
Korzynska, Anna
Jaén, Joaquín
García-Rojo, Marcial
Bueno, Gloria
Salvadó, Mª Teresa
Álvaro, Tomás
Oños, Montse
Fernández-Carrobles, María del Milagro
Llobera, Montserrat
Baucells, Jordi
Orero, Guifré
Lejeune, Marylène
author_sort López, Carlos
collection PubMed
description INTRODUCTION: Lymph nodes are one of the main sites where an effective immune response develops. Normally, axillary nodes are the first place where breast cancer produces metastases. Several studies have demonstrated the importance of immune cells, especially dendritic cells, in the evolution of breast cancer. The goal of the project is to identify differences in the patterns of immune infiltrates, with particular emphasis on dendritic cells, in tumour and axillary node biopsies between patients with and without metastases in the axillary nodes at the time of diagnosis. It is expected that these differences will be able to explain differences in survival, relapse and clinicopathological variables between the two groups. METHODS AND ANALYSIS: The study will involve 100 patients diagnosed with invasive breast cancer between 2000 and 2007, 50% of whom have metastases in the axillary lymph node at diagnosis. In selected patients, two cylinders from biopsies of representative areas of tumour and axillary nodes (with and without metastasis) will be selected and organised in tissue microarrays. Samples will be stained using immunohistochemical techniques for different markers of immune response and dendritic cells. Two images of each cylinder will be captured under standardised conditions for each marker. Each marker will be quantified automatically by digital image procedures using Image-Pro Plus and Image-J software. Associations of survival, relapse and other clinicopathological variables with the automatically quantified levels of immune infiltrates in patients with and without axillary node metastasis will be sought. ETHICS AND DISSEMINATION: The present project has been approved by the Clinical Research Ethics Committee of the Hospital Universitari Joan XXIII (Ref: 22p/2011). Those patients whose biopsies and clinical data are to be used will give their signed informed consent. Results will be published in peer-reviewed journals.
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spelling pubmed-41279222014-08-12 Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study López, Carlos Callau, Cristina Bosch, Ramon Korzynska, Anna Jaén, Joaquín García-Rojo, Marcial Bueno, Gloria Salvadó, Mª Teresa Álvaro, Tomás Oños, Montse Fernández-Carrobles, María del Milagro Llobera, Montserrat Baucells, Jordi Orero, Guifré Lejeune, Marylène BMJ Open Pathology INTRODUCTION: Lymph nodes are one of the main sites where an effective immune response develops. Normally, axillary nodes are the first place where breast cancer produces metastases. Several studies have demonstrated the importance of immune cells, especially dendritic cells, in the evolution of breast cancer. The goal of the project is to identify differences in the patterns of immune infiltrates, with particular emphasis on dendritic cells, in tumour and axillary node biopsies between patients with and without metastases in the axillary nodes at the time of diagnosis. It is expected that these differences will be able to explain differences in survival, relapse and clinicopathological variables between the two groups. METHODS AND ANALYSIS: The study will involve 100 patients diagnosed with invasive breast cancer between 2000 and 2007, 50% of whom have metastases in the axillary lymph node at diagnosis. In selected patients, two cylinders from biopsies of representative areas of tumour and axillary nodes (with and without metastasis) will be selected and organised in tissue microarrays. Samples will be stained using immunohistochemical techniques for different markers of immune response and dendritic cells. Two images of each cylinder will be captured under standardised conditions for each marker. Each marker will be quantified automatically by digital image procedures using Image-Pro Plus and Image-J software. Associations of survival, relapse and other clinicopathological variables with the automatically quantified levels of immune infiltrates in patients with and without axillary node metastasis will be sought. ETHICS AND DISSEMINATION: The present project has been approved by the Clinical Research Ethics Committee of the Hospital Universitari Joan XXIII (Ref: 22p/2011). Those patients whose biopsies and clinical data are to be used will give their signed informed consent. Results will be published in peer-reviewed journals. BMJ Publishing Group 2014-08-04 /pmc/articles/PMC4127922/ /pubmed/25091015 http://dx.doi.org/10.1136/bmjopen-2014-005643 Text en Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Pathology
López, Carlos
Callau, Cristina
Bosch, Ramon
Korzynska, Anna
Jaén, Joaquín
García-Rojo, Marcial
Bueno, Gloria
Salvadó, Mª Teresa
Álvaro, Tomás
Oños, Montse
Fernández-Carrobles, María del Milagro
Llobera, Montserrat
Baucells, Jordi
Orero, Guifré
Lejeune, Marylène
Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study
title Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study
title_full Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study
title_fullStr Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study
title_full_unstemmed Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study
title_short Development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study
title_sort development of automated quantification methodologies of immunohistochemical markers to determine patterns of immune response in breast cancer: a retrospective cohort study
topic Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4127922/
https://www.ncbi.nlm.nih.gov/pubmed/25091015
http://dx.doi.org/10.1136/bmjopen-2014-005643
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