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The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer

OBJECTIVES: The epithelial-mesenchymal-transition (EMT) is an important step in the invasion and metastasis of cancer. A critical molecular feature of this process is the downregulation of the E-cadherin expression, which is primarily controlled by Snail-related zinc-finger transcription factors. Th...

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Autores principales: Takai, Masaaki, Terai, Yoshito, Kawaguchi, Hiroshi, Ashihara, Keisuke, Fujiwara, Satoe, Tanaka, Tomohito, Tsunetoh, Satoshi, Tanaka, Yoshimichi, Sasaki, Hiroshi, Kanemura, Masanori, Tanabe, Akiko, Ohmichi, Masahide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4127950/
https://www.ncbi.nlm.nih.gov/pubmed/25296567
http://dx.doi.org/10.1186/1757-2215-7-76
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author Takai, Masaaki
Terai, Yoshito
Kawaguchi, Hiroshi
Ashihara, Keisuke
Fujiwara, Satoe
Tanaka, Tomohito
Tsunetoh, Satoshi
Tanaka, Yoshimichi
Sasaki, Hiroshi
Kanemura, Masanori
Tanabe, Akiko
Ohmichi, Masahide
author_facet Takai, Masaaki
Terai, Yoshito
Kawaguchi, Hiroshi
Ashihara, Keisuke
Fujiwara, Satoe
Tanaka, Tomohito
Tsunetoh, Satoshi
Tanaka, Yoshimichi
Sasaki, Hiroshi
Kanemura, Masanori
Tanabe, Akiko
Ohmichi, Masahide
author_sort Takai, Masaaki
collection PubMed
description OBJECTIVES: The epithelial-mesenchymal-transition (EMT) is an important step in the invasion and metastasis of cancer. A critical molecular feature of this process is the downregulation of the E-cadherin expression, which is primarily controlled by Snail-related zinc-finger transcription factors. The aim of this study was to evaluate the prognostic impact of the expression of EMT-related proteins (E-cadherin and Snail) in patients with ovarian cancer. METHODS: An immunohistochemical analysis was conducted using tissue microarray samples of 174 primary tumors and 34 metastases of ovarian carcinoma, and the relationships between the protein expression, clinicopathological features and outcomes were investigated. RESULTS: A reduced E-cadherin expression was observed in 36.8% of the primary tumors and 30.4%, 35.7%, 37.7% and 52.7% of the stage I, II, III and IV tumors, respectively. The nuclear expression of Snail was positive in 33.9% of the primary tumors. The rate of an EMT-positive status, as represented by both a reduced E-cadherin expression and a nuclear expression of Snail, was significantly higher in the patients with peritoneal dissemination than in those without (p < 0.05). The EMT status was significantly associated with both the progression-free survival and overall survival (p <0.01). A multivariate analysis showed an EMT-positive status to be a significant predictor of both the progression-free survival (p < 0.05) and overall survival (P < 0.01). CONCLUSIONS: These data indicate that the EMT status is significantly associated with peritoneal metastasis and both the progression-free survival and overall survival in patients with ovarian cancer. Therefore, clarifying and controlling EMT signaling is a promising approach to molecular targeted therapy for ovarian cancer.
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spelling pubmed-41279502014-08-12 The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer Takai, Masaaki Terai, Yoshito Kawaguchi, Hiroshi Ashihara, Keisuke Fujiwara, Satoe Tanaka, Tomohito Tsunetoh, Satoshi Tanaka, Yoshimichi Sasaki, Hiroshi Kanemura, Masanori Tanabe, Akiko Ohmichi, Masahide J Ovarian Res Research OBJECTIVES: The epithelial-mesenchymal-transition (EMT) is an important step in the invasion and metastasis of cancer. A critical molecular feature of this process is the downregulation of the E-cadherin expression, which is primarily controlled by Snail-related zinc-finger transcription factors. The aim of this study was to evaluate the prognostic impact of the expression of EMT-related proteins (E-cadherin and Snail) in patients with ovarian cancer. METHODS: An immunohistochemical analysis was conducted using tissue microarray samples of 174 primary tumors and 34 metastases of ovarian carcinoma, and the relationships between the protein expression, clinicopathological features and outcomes were investigated. RESULTS: A reduced E-cadherin expression was observed in 36.8% of the primary tumors and 30.4%, 35.7%, 37.7% and 52.7% of the stage I, II, III and IV tumors, respectively. The nuclear expression of Snail was positive in 33.9% of the primary tumors. The rate of an EMT-positive status, as represented by both a reduced E-cadherin expression and a nuclear expression of Snail, was significantly higher in the patients with peritoneal dissemination than in those without (p < 0.05). The EMT status was significantly associated with both the progression-free survival and overall survival (p <0.01). A multivariate analysis showed an EMT-positive status to be a significant predictor of both the progression-free survival (p < 0.05) and overall survival (P < 0.01). CONCLUSIONS: These data indicate that the EMT status is significantly associated with peritoneal metastasis and both the progression-free survival and overall survival in patients with ovarian cancer. Therefore, clarifying and controlling EMT signaling is a promising approach to molecular targeted therapy for ovarian cancer. BioMed Central 2014-07-27 /pmc/articles/PMC4127950/ /pubmed/25296567 http://dx.doi.org/10.1186/1757-2215-7-76 Text en Copyright © 2014 Takai et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Takai, Masaaki
Terai, Yoshito
Kawaguchi, Hiroshi
Ashihara, Keisuke
Fujiwara, Satoe
Tanaka, Tomohito
Tsunetoh, Satoshi
Tanaka, Yoshimichi
Sasaki, Hiroshi
Kanemura, Masanori
Tanabe, Akiko
Ohmichi, Masahide
The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer
title The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer
title_full The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer
title_fullStr The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer
title_full_unstemmed The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer
title_short The EMT (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer
title_sort emt (epithelial-mesenchymal-transition)-related protein expression indicates the metastatic status and prognosis in patients with ovarian cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4127950/
https://www.ncbi.nlm.nih.gov/pubmed/25296567
http://dx.doi.org/10.1186/1757-2215-7-76
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