Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients

Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in t...

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Detalles Bibliográficos
Autores principales: Della Vittoria Scarpati, Giuseppina, Calura, Enrica, Di Marino, Mariacristina, Romualdi, Chiara, Beltrame, Luca, Malapelle, Umberto, Troncone, Giancarlo, De Stefano, Alfonso, Pepe, Stefano, De Placido, Sabino, D'Incalci, Maurizio, Marchini, Sergio, Carlomagno, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128171/
https://www.ncbi.nlm.nih.gov/pubmed/25143946
http://dx.doi.org/10.1155/2014/840921
Descripción
Sumario:Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.

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