Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients

Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in t...

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Autores principales: Della Vittoria Scarpati, Giuseppina, Calura, Enrica, Di Marino, Mariacristina, Romualdi, Chiara, Beltrame, Luca, Malapelle, Umberto, Troncone, Giancarlo, De Stefano, Alfonso, Pepe, Stefano, De Placido, Sabino, D'Incalci, Maurizio, Marchini, Sergio, Carlomagno, Chiara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128171/
https://www.ncbi.nlm.nih.gov/pubmed/25143946
http://dx.doi.org/10.1155/2014/840921
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author Della Vittoria Scarpati, Giuseppina
Calura, Enrica
Di Marino, Mariacristina
Romualdi, Chiara
Beltrame, Luca
Malapelle, Umberto
Troncone, Giancarlo
De Stefano, Alfonso
Pepe, Stefano
De Placido, Sabino
D'Incalci, Maurizio
Marchini, Sergio
Carlomagno, Chiara
author_facet Della Vittoria Scarpati, Giuseppina
Calura, Enrica
Di Marino, Mariacristina
Romualdi, Chiara
Beltrame, Luca
Malapelle, Umberto
Troncone, Giancarlo
De Stefano, Alfonso
Pepe, Stefano
De Placido, Sabino
D'Incalci, Maurizio
Marchini, Sergio
Carlomagno, Chiara
author_sort Della Vittoria Scarpati, Giuseppina
collection PubMed
description Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival.
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spelling pubmed-41281712014-08-20 Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients Della Vittoria Scarpati, Giuseppina Calura, Enrica Di Marino, Mariacristina Romualdi, Chiara Beltrame, Luca Malapelle, Umberto Troncone, Giancarlo De Stefano, Alfonso Pepe, Stefano De Placido, Sabino D'Incalci, Maurizio Marchini, Sergio Carlomagno, Chiara Biomed Res Int Research Article Microarray technology was used to profile miRNA expression in primary tumor and stromal tissue from paraffin embedded material of 51 patients with colorectal cancer. 26 miRNAs resulted differentially expressed with at least 2-fold change in tumor tissue with respect to stroma (16 more expressed in the tumor and 10 more expressed in the stroma). 10/26 were confirmed as differentially expressed at qRTPCR: miR-200c-3p, miR-141-3p, miR-200b-3p, miR-200a-3p, miR-1246, miR-92a-3p, miR-194-5p, miR-192-5p, miR-3651-5p, and miR-574-3p. No significant association was found between miRNA expressions and stage at diagnosis, site of primary tumor, first site of metastasis, progression-free, or overall survival. Hindawi Publishing Corporation 2014 2014-07-10 /pmc/articles/PMC4128171/ /pubmed/25143946 http://dx.doi.org/10.1155/2014/840921 Text en Copyright © 2014 Giuseppina Della Vittoria Scarpati et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Della Vittoria Scarpati, Giuseppina
Calura, Enrica
Di Marino, Mariacristina
Romualdi, Chiara
Beltrame, Luca
Malapelle, Umberto
Troncone, Giancarlo
De Stefano, Alfonso
Pepe, Stefano
De Placido, Sabino
D'Incalci, Maurizio
Marchini, Sergio
Carlomagno, Chiara
Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients
title Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients
title_full Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients
title_fullStr Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients
title_full_unstemmed Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients
title_short Analysis of Differential miRNA Expression in Primary Tumor and Stroma of Colorectal Cancer Patients
title_sort analysis of differential mirna expression in primary tumor and stroma of colorectal cancer patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128171/
https://www.ncbi.nlm.nih.gov/pubmed/25143946
http://dx.doi.org/10.1155/2014/840921
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