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Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield
Mammary gland is made up of a branching network of ducts that end with alveoli which surrounds the lumen. These alveolar mammary epithelial cells (MEC) reflect the milk producing ability of farm animals. In this study, we have used 2D-DIGE and mass spectrometry to identify the protein changes in MEC...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128602/ https://www.ncbi.nlm.nih.gov/pubmed/25111801 http://dx.doi.org/10.1371/journal.pone.0102515 |
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author | Janjanam, Jagadeesh Singh, Surender Jena, Manoj K. Varshney, Nishant Kola, Srujana Kumar, Sudarshan Kaushik, Jai K. Grover, Sunita Dang, Ajay K. Mukesh, Manishi Prakash, B. S. Mohanty, Ashok K. |
author_facet | Janjanam, Jagadeesh Singh, Surender Jena, Manoj K. Varshney, Nishant Kola, Srujana Kumar, Sudarshan Kaushik, Jai K. Grover, Sunita Dang, Ajay K. Mukesh, Manishi Prakash, B. S. Mohanty, Ashok K. |
author_sort | Janjanam, Jagadeesh |
collection | PubMed |
description | Mammary gland is made up of a branching network of ducts that end with alveoli which surrounds the lumen. These alveolar mammary epithelial cells (MEC) reflect the milk producing ability of farm animals. In this study, we have used 2D-DIGE and mass spectrometry to identify the protein changes in MEC during immediate early, peak and late stages of lactation and also compared differentially expressed proteins in MEC isolated from milk of high and low milk producing cows. We have identified 41 differentially expressed proteins during lactation stages and 22 proteins in high and low milk yielding cows. Bioinformatics analysis showed that a majority of the differentially expressed proteins are associated in metabolic process, catalytic and binding activity. The differentially expressed proteins were mapped to the available biological pathways and networks involved in lactation. The proteins up-regulated during late stage of lactation are associated with NF-κB stress induced signaling pathways and whereas Akt, PI3K and p38/MAPK signaling pathways are associated with high milk production mediated through insulin hormone signaling. |
format | Online Article Text |
id | pubmed-4128602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41286022014-08-12 Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield Janjanam, Jagadeesh Singh, Surender Jena, Manoj K. Varshney, Nishant Kola, Srujana Kumar, Sudarshan Kaushik, Jai K. Grover, Sunita Dang, Ajay K. Mukesh, Manishi Prakash, B. S. Mohanty, Ashok K. PLoS One Research Article Mammary gland is made up of a branching network of ducts that end with alveoli which surrounds the lumen. These alveolar mammary epithelial cells (MEC) reflect the milk producing ability of farm animals. In this study, we have used 2D-DIGE and mass spectrometry to identify the protein changes in MEC during immediate early, peak and late stages of lactation and also compared differentially expressed proteins in MEC isolated from milk of high and low milk producing cows. We have identified 41 differentially expressed proteins during lactation stages and 22 proteins in high and low milk yielding cows. Bioinformatics analysis showed that a majority of the differentially expressed proteins are associated in metabolic process, catalytic and binding activity. The differentially expressed proteins were mapped to the available biological pathways and networks involved in lactation. The proteins up-regulated during late stage of lactation are associated with NF-κB stress induced signaling pathways and whereas Akt, PI3K and p38/MAPK signaling pathways are associated with high milk production mediated through insulin hormone signaling. Public Library of Science 2014-08-11 /pmc/articles/PMC4128602/ /pubmed/25111801 http://dx.doi.org/10.1371/journal.pone.0102515 Text en © 2014 Janjanam et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Janjanam, Jagadeesh Singh, Surender Jena, Manoj K. Varshney, Nishant Kola, Srujana Kumar, Sudarshan Kaushik, Jai K. Grover, Sunita Dang, Ajay K. Mukesh, Manishi Prakash, B. S. Mohanty, Ashok K. Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield |
title | Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield |
title_full | Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield |
title_fullStr | Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield |
title_full_unstemmed | Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield |
title_short | Comparative 2D-DIGE Proteomic Analysis of Bovine Mammary Epithelial Cells during Lactation Reveals Protein Signatures for Lactation Persistency and Milk Yield |
title_sort | comparative 2d-dige proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128602/ https://www.ncbi.nlm.nih.gov/pubmed/25111801 http://dx.doi.org/10.1371/journal.pone.0102515 |
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