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Identification of gene-based responses in human blood cells exposed to alpha particle radiation
BACKGROUND: The threat of a terrorist-precipitated nuclear event places humans at danger for radiological exposures. Isotopes which emit alpha (α)-particle radiation pose the highest risk. Currently, gene expression signatures are being developed for radiation biodosimetry and triage with respect to...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128605/ https://www.ncbi.nlm.nih.gov/pubmed/25017500 http://dx.doi.org/10.1186/1755-8794-7-43 |
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author | Chauhan, Vinita Howland, Matthew Wilkins, Ruth |
author_facet | Chauhan, Vinita Howland, Matthew Wilkins, Ruth |
author_sort | Chauhan, Vinita |
collection | PubMed |
description | BACKGROUND: The threat of a terrorist-precipitated nuclear event places humans at danger for radiological exposures. Isotopes which emit alpha (α)-particle radiation pose the highest risk. Currently, gene expression signatures are being developed for radiation biodosimetry and triage with respect to ionizing photon radiation. This study was designed to determine if similar gene expression profiles are obtained after exposures involving α-particles. METHODS: Peripheral blood mononuclear cells (PBMCs) were used to identify sensitive and robust gene-based biomarkers of α-particle radiation exposure. Cells were isolated from healthy individuals and were irradiated at doses ranging from 0-1.5 Gy. Microarray technology was employed to identify transcripts that were differentially expressed relative to unirradiated cells 24 hours post-exposure. Statistical analysis identified modulated genes at each of the individual doses. RESULTS: Twenty-nine genes were common to all doses with expression levels ranging from 2-10 fold relative to control treatment group. This subset of genes was further assessed in independent complete white blood cell (WBC) populations exposed to either α-particles or X-rays using quantitative real-time PCR. This 29 gene panel was responsive in the α-particle exposed WBCs and was shown to exhibit differential fold-changes compared to X-irradiated cells, though no α-particle specific transcripts were identified. CONCLUSION: Current gene panels for photon radiation may also be applicable for use in α-particle radiation biodosimetry. |
format | Online Article Text |
id | pubmed-4128605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41286052014-08-12 Identification of gene-based responses in human blood cells exposed to alpha particle radiation Chauhan, Vinita Howland, Matthew Wilkins, Ruth BMC Med Genomics Research Article BACKGROUND: The threat of a terrorist-precipitated nuclear event places humans at danger for radiological exposures. Isotopes which emit alpha (α)-particle radiation pose the highest risk. Currently, gene expression signatures are being developed for radiation biodosimetry and triage with respect to ionizing photon radiation. This study was designed to determine if similar gene expression profiles are obtained after exposures involving α-particles. METHODS: Peripheral blood mononuclear cells (PBMCs) were used to identify sensitive and robust gene-based biomarkers of α-particle radiation exposure. Cells were isolated from healthy individuals and were irradiated at doses ranging from 0-1.5 Gy. Microarray technology was employed to identify transcripts that were differentially expressed relative to unirradiated cells 24 hours post-exposure. Statistical analysis identified modulated genes at each of the individual doses. RESULTS: Twenty-nine genes were common to all doses with expression levels ranging from 2-10 fold relative to control treatment group. This subset of genes was further assessed in independent complete white blood cell (WBC) populations exposed to either α-particles or X-rays using quantitative real-time PCR. This 29 gene panel was responsive in the α-particle exposed WBCs and was shown to exhibit differential fold-changes compared to X-irradiated cells, though no α-particle specific transcripts were identified. CONCLUSION: Current gene panels for photon radiation may also be applicable for use in α-particle radiation biodosimetry. BioMed Central 2014-07-12 /pmc/articles/PMC4128605/ /pubmed/25017500 http://dx.doi.org/10.1186/1755-8794-7-43 Text en Copyright © 2014 Chauhan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Chauhan, Vinita Howland, Matthew Wilkins, Ruth Identification of gene-based responses in human blood cells exposed to alpha particle radiation |
title | Identification of gene-based responses in human blood cells exposed to alpha particle radiation |
title_full | Identification of gene-based responses in human blood cells exposed to alpha particle radiation |
title_fullStr | Identification of gene-based responses in human blood cells exposed to alpha particle radiation |
title_full_unstemmed | Identification of gene-based responses in human blood cells exposed to alpha particle radiation |
title_short | Identification of gene-based responses in human blood cells exposed to alpha particle radiation |
title_sort | identification of gene-based responses in human blood cells exposed to alpha particle radiation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128605/ https://www.ncbi.nlm.nih.gov/pubmed/25017500 http://dx.doi.org/10.1186/1755-8794-7-43 |
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