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An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection
Several critical ocular diseases that can lead to blindness are due to retinal disorders. Subretinal drug delivery has been developed recently for the treatment of retinal disorders such as hemorrhage because of the specific ocular structure, namely, the blood retinal barrier (BRB). In the present s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128757/ https://www.ncbi.nlm.nih.gov/pubmed/25111562 http://dx.doi.org/10.1371/journal.pone.0104145 |
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author | You, Yong Sung Lee, Chang Yeol Li, Chengguo Lee, Sung Ho Kim, Kibom Jung, Hyungil |
author_facet | You, Yong Sung Lee, Chang Yeol Li, Chengguo Lee, Sung Ho Kim, Kibom Jung, Hyungil |
author_sort | You, Yong Sung |
collection | PubMed |
description | Several critical ocular diseases that can lead to blindness are due to retinal disorders. Subretinal drug delivery has been developed recently for the treatment of retinal disorders such as hemorrhage because of the specific ocular structure, namely, the blood retinal barrier (BRB). In the present study, we developed an Arched Micro-injector (ARCMI) for subretinal drug delivery with minimal retinal tissue damage. ARCMIs were fabricated using three major techniques: reverse drawing lithography, controlled air flow, and electroplating. In order to achieve minimal retinal tissue damage, ARCMIs were fabricated with specific features such as a 0.15 mm(−1) curvature, 45° tip bevel, 5 mm length, inner diameter of 40 µm, and an outer diameter of 100 µm. These specific features were optimized via in-vitro experiments in artificial ocular hemispherical structures and subretinal injection of indocyanine green in porcine eye ex-vivo. We confirmed that the ARCMI was capable of delivering ocular drugs by subretinal injection without unusual subretinal tissue damage, including hemorrhage. |
format | Online Article Text |
id | pubmed-4128757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41287572014-08-12 An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection You, Yong Sung Lee, Chang Yeol Li, Chengguo Lee, Sung Ho Kim, Kibom Jung, Hyungil PLoS One Research Article Several critical ocular diseases that can lead to blindness are due to retinal disorders. Subretinal drug delivery has been developed recently for the treatment of retinal disorders such as hemorrhage because of the specific ocular structure, namely, the blood retinal barrier (BRB). In the present study, we developed an Arched Micro-injector (ARCMI) for subretinal drug delivery with minimal retinal tissue damage. ARCMIs were fabricated using three major techniques: reverse drawing lithography, controlled air flow, and electroplating. In order to achieve minimal retinal tissue damage, ARCMIs were fabricated with specific features such as a 0.15 mm(−1) curvature, 45° tip bevel, 5 mm length, inner diameter of 40 µm, and an outer diameter of 100 µm. These specific features were optimized via in-vitro experiments in artificial ocular hemispherical structures and subretinal injection of indocyanine green in porcine eye ex-vivo. We confirmed that the ARCMI was capable of delivering ocular drugs by subretinal injection without unusual subretinal tissue damage, including hemorrhage. Public Library of Science 2014-08-11 /pmc/articles/PMC4128757/ /pubmed/25111562 http://dx.doi.org/10.1371/journal.pone.0104145 Text en © 2014 You et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article You, Yong Sung Lee, Chang Yeol Li, Chengguo Lee, Sung Ho Kim, Kibom Jung, Hyungil An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection |
title | An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection |
title_full | An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection |
title_fullStr | An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection |
title_full_unstemmed | An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection |
title_short | An Arched Micro-Injector (ARCMI) for Innocuous Subretinal Injection |
title_sort | arched micro-injector (arcmi) for innocuous subretinal injection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4128757/ https://www.ncbi.nlm.nih.gov/pubmed/25111562 http://dx.doi.org/10.1371/journal.pone.0104145 |
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