Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen

Naturally occurring fragments of the abundant semen proteins prostatic acid phosphatase (PAP) and semenogelins form amyloid fibrils in vitro. These fibrils boost HIV infection and may play a key role in the spread of the AIDS pandemic. However, the presence of amyloid fibrils in semen remained to be...

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Autores principales: Usmani, Shariq M., Zirafi, Onofrio, Müller, Janis, Sandi-Monroy, Nathallie, Yadav, Jay K., Meier, Christoph, Weil, Tanja, Roan, Nadia R., Greene, Warner C., Walther, Paul, Nilsson, K. Peter R., Hammarström, Per, Wetzel, Ronald, Pilcher, Christopher D., Gagsteiger, Friedrich, Fändrich, Marcus, Kirchhoff, Frank, Münch, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129123/
https://www.ncbi.nlm.nih.gov/pubmed/24691351
http://dx.doi.org/10.1038/ncomms4508
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author Usmani, Shariq M.
Zirafi, Onofrio
Müller, Janis
Sandi-Monroy, Nathallie
Yadav, Jay K.
Meier, Christoph
Weil, Tanja
Roan, Nadia R.
Greene, Warner C.
Walther, Paul
Nilsson, K. Peter R.
Hammarström, Per
Wetzel, Ronald
Pilcher, Christopher D.
Gagsteiger, Friedrich
Fändrich, Marcus
Kirchhoff, Frank
Münch, Jan
author_facet Usmani, Shariq M.
Zirafi, Onofrio
Müller, Janis
Sandi-Monroy, Nathallie
Yadav, Jay K.
Meier, Christoph
Weil, Tanja
Roan, Nadia R.
Greene, Warner C.
Walther, Paul
Nilsson, K. Peter R.
Hammarström, Per
Wetzel, Ronald
Pilcher, Christopher D.
Gagsteiger, Friedrich
Fändrich, Marcus
Kirchhoff, Frank
Münch, Jan
author_sort Usmani, Shariq M.
collection PubMed
description Naturally occurring fragments of the abundant semen proteins prostatic acid phosphatase (PAP) and semenogelins form amyloid fibrils in vitro. These fibrils boost HIV infection and may play a key role in the spread of the AIDS pandemic. However, the presence of amyloid fibrils in semen remained to be demonstrated. Here, we use state of the art confocal and electron microscopy techniques for direct imaging of amyloid fibrils in human ejaculates. We detect amyloid aggregates in all semen samples and find that they partially consist of PAP fragments, interact with HIV particles and increase viral infectivity. Our results establish semen as a body fluid that naturally contains amyloid fibrils that are exploited by HIV to promote its sexual transmission.
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spelling pubmed-41291232014-10-01 Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen Usmani, Shariq M. Zirafi, Onofrio Müller, Janis Sandi-Monroy, Nathallie Yadav, Jay K. Meier, Christoph Weil, Tanja Roan, Nadia R. Greene, Warner C. Walther, Paul Nilsson, K. Peter R. Hammarström, Per Wetzel, Ronald Pilcher, Christopher D. Gagsteiger, Friedrich Fändrich, Marcus Kirchhoff, Frank Münch, Jan Nat Commun Article Naturally occurring fragments of the abundant semen proteins prostatic acid phosphatase (PAP) and semenogelins form amyloid fibrils in vitro. These fibrils boost HIV infection and may play a key role in the spread of the AIDS pandemic. However, the presence of amyloid fibrils in semen remained to be demonstrated. Here, we use state of the art confocal and electron microscopy techniques for direct imaging of amyloid fibrils in human ejaculates. We detect amyloid aggregates in all semen samples and find that they partially consist of PAP fragments, interact with HIV particles and increase viral infectivity. Our results establish semen as a body fluid that naturally contains amyloid fibrils that are exploited by HIV to promote its sexual transmission. 2014-04-01 /pmc/articles/PMC4129123/ /pubmed/24691351 http://dx.doi.org/10.1038/ncomms4508 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Usmani, Shariq M.
Zirafi, Onofrio
Müller, Janis
Sandi-Monroy, Nathallie
Yadav, Jay K.
Meier, Christoph
Weil, Tanja
Roan, Nadia R.
Greene, Warner C.
Walther, Paul
Nilsson, K. Peter R.
Hammarström, Per
Wetzel, Ronald
Pilcher, Christopher D.
Gagsteiger, Friedrich
Fändrich, Marcus
Kirchhoff, Frank
Münch, Jan
Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen
title Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen
title_full Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen
title_fullStr Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen
title_full_unstemmed Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen
title_short Direct visualization of HIV-enhancing endogenous amyloid fibrils in human semen
title_sort direct visualization of hiv-enhancing endogenous amyloid fibrils in human semen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129123/
https://www.ncbi.nlm.nih.gov/pubmed/24691351
http://dx.doi.org/10.1038/ncomms4508
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