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Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi

Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individual Paramphistomum cervi were determined for the first time. The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2),...

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Autores principales: Zheng, Xu, Chang, Qiao-Cheng, Zhang, Yan, Tian, Si-Qin, Lou, Yan, Duan, Hong, Guo, Dong-Hui, Wang, Chun-Ren, Zhu, Xing-Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129167/
https://www.ncbi.nlm.nih.gov/pubmed/25140347
http://dx.doi.org/10.1155/2014/751907
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author Zheng, Xu
Chang, Qiao-Cheng
Zhang, Yan
Tian, Si-Qin
Lou, Yan
Duan, Hong
Guo, Dong-Hui
Wang, Chun-Ren
Zhu, Xing-Quan
author_facet Zheng, Xu
Chang, Qiao-Cheng
Zhang, Yan
Tian, Si-Qin
Lou, Yan
Duan, Hong
Guo, Dong-Hui
Wang, Chun-Ren
Zhu, Xing-Quan
author_sort Zheng, Xu
collection PubMed
description Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individual Paramphistomum cervi were determined for the first time. The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2), the 28S rDNA, and the intergenic spacer (IGS) regions, had a length range of 8,493–10,221 bp. The lengths of the investigated 18S, ITS1, 5.8S, ITS2, and 28S rDNA sequences, which were 1,994 bp, 1,293 bp, 157 bp, 286 bp, and 4,186 bp, respectively, did not vary. However, the IGS rDNA sequences had a length range of 577–2,305 bp. The 5.8S and ITS-2 rDNA sequences had 100% identity among the five investigated samples, while the identities among the IGS had a range of 53.7–99.8%. A comparative analysis revealed that different types and numbers of repeats were found within each ITS1 and IGS region, which may be related to the length polymorphism of IGS. The phylogenetic position of P. cervi in Paramphistomatidae was analyzed based on the 18S rDNA sequences. These results will aid in studying the intra- and interspecific variation of the Paramphistomatidae and the systematics and phylogenetics of Digenea.
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spelling pubmed-41291672014-08-19 Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi Zheng, Xu Chang, Qiao-Cheng Zhang, Yan Tian, Si-Qin Lou, Yan Duan, Hong Guo, Dong-Hui Wang, Chun-Ren Zhu, Xing-Quan ScientificWorldJournal Research Article Sequences of the complete nuclear ribosomal DNA (rDNA) gene from five individual Paramphistomum cervi were determined for the first time. The five complete rDNA sequences, which included the 18S rDNA, the internal transcribed spacer 1 (ITS1), the 5.8S rDNA, the internal transcribed spacer 2 (ITS2), the 28S rDNA, and the intergenic spacer (IGS) regions, had a length range of 8,493–10,221 bp. The lengths of the investigated 18S, ITS1, 5.8S, ITS2, and 28S rDNA sequences, which were 1,994 bp, 1,293 bp, 157 bp, 286 bp, and 4,186 bp, respectively, did not vary. However, the IGS rDNA sequences had a length range of 577–2,305 bp. The 5.8S and ITS-2 rDNA sequences had 100% identity among the five investigated samples, while the identities among the IGS had a range of 53.7–99.8%. A comparative analysis revealed that different types and numbers of repeats were found within each ITS1 and IGS region, which may be related to the length polymorphism of IGS. The phylogenetic position of P. cervi in Paramphistomatidae was analyzed based on the 18S rDNA sequences. These results will aid in studying the intra- and interspecific variation of the Paramphistomatidae and the systematics and phylogenetics of Digenea. Hindawi Publishing Corporation 2014 2014-07-20 /pmc/articles/PMC4129167/ /pubmed/25140347 http://dx.doi.org/10.1155/2014/751907 Text en Copyright © 2014 Xu Zheng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zheng, Xu
Chang, Qiao-Cheng
Zhang, Yan
Tian, Si-Qin
Lou, Yan
Duan, Hong
Guo, Dong-Hui
Wang, Chun-Ren
Zhu, Xing-Quan
Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi
title Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi
title_full Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi
title_fullStr Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi
title_full_unstemmed Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi
title_short Characterization of the Complete Nuclear Ribosomal DNA Sequences of Paramphistomum cervi
title_sort characterization of the complete nuclear ribosomal dna sequences of paramphistomum cervi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129167/
https://www.ncbi.nlm.nih.gov/pubmed/25140347
http://dx.doi.org/10.1155/2014/751907
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