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Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice
To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respir...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129438/ https://www.ncbi.nlm.nih.gov/pubmed/25027440 http://dx.doi.org/10.7554/eLife.03427 |
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author | Wang, Xueying Hayes, John A Revill, Ann L Song, Hanbing Kottick, Andrew Vann, Nikolas C LaMar, M Drew Picardo, Maria Cristina D Akins, Victoria T Funk, Gregory D Del Negro, Christopher A |
author_facet | Wang, Xueying Hayes, John A Revill, Ann L Song, Hanbing Kottick, Andrew Vann, Nikolas C LaMar, M Drew Picardo, Maria Cristina D Akins, Victoria T Funk, Gregory D Del Negro, Christopher A |
author_sort | Wang, Xueying |
collection | PubMed |
description | To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm generator in mammals are derived from Dbx1-expressing precursors. Here, we show that selective photonic destruction of Dbx1 preBötC neurons in neonatal mouse slices impairs respiratory rhythm but surprisingly also the magnitude of motor output; respiratory hypoglossal nerve discharge decreased and its frequency steadily diminished until rhythm stopped irreversibly after 85±20 (mean ± SEM) cellular ablations, which corresponds to ∼15% of the estimated population. These results demonstrate that a single canonical interneuron class generates respiratory rhythm and contributes in a premotor capacity, whereas these functions are normally attributed to discrete populations. We also establish quantitative cellular parameters that govern network viability, which may have ramifications for respiratory pathology in disease states. DOI: http://dx.doi.org/10.7554/eLife.03427.001 |
format | Online Article Text |
id | pubmed-4129438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41294382014-08-22 Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice Wang, Xueying Hayes, John A Revill, Ann L Song, Hanbing Kottick, Andrew Vann, Nikolas C LaMar, M Drew Picardo, Maria Cristina D Akins, Victoria T Funk, Gregory D Del Negro, Christopher A eLife Neuroscience To understand the neural origins of rhythmic behavior one must characterize the central pattern generator circuit and quantify the population size needed to sustain functionality. Breathing-related interneurons of the brainstem pre-Bötzinger complex (preBötC) that putatively comprise the core respiratory rhythm generator in mammals are derived from Dbx1-expressing precursors. Here, we show that selective photonic destruction of Dbx1 preBötC neurons in neonatal mouse slices impairs respiratory rhythm but surprisingly also the magnitude of motor output; respiratory hypoglossal nerve discharge decreased and its frequency steadily diminished until rhythm stopped irreversibly after 85±20 (mean ± SEM) cellular ablations, which corresponds to ∼15% of the estimated population. These results demonstrate that a single canonical interneuron class generates respiratory rhythm and contributes in a premotor capacity, whereas these functions are normally attributed to discrete populations. We also establish quantitative cellular parameters that govern network viability, which may have ramifications for respiratory pathology in disease states. DOI: http://dx.doi.org/10.7554/eLife.03427.001 eLife Sciences Publications, Ltd 2014-07-15 /pmc/articles/PMC4129438/ /pubmed/25027440 http://dx.doi.org/10.7554/eLife.03427 Text en Copyright © 2014, Wang et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Wang, Xueying Hayes, John A Revill, Ann L Song, Hanbing Kottick, Andrew Vann, Nikolas C LaMar, M Drew Picardo, Maria Cristina D Akins, Victoria T Funk, Gregory D Del Negro, Christopher A Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice |
title | Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice |
title_full | Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice |
title_fullStr | Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice |
title_full_unstemmed | Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice |
title_short | Laser ablation of Dbx1 neurons in the pre-Bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice |
title_sort | laser ablation of dbx1 neurons in the pre-bötzinger complex stops inspiratory rhythm and impairs output in neonatal mice |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129438/ https://www.ncbi.nlm.nih.gov/pubmed/25027440 http://dx.doi.org/10.7554/eLife.03427 |
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