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Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (R(L)) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inf...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129625/ https://www.ncbi.nlm.nih.gov/pubmed/25161620 http://dx.doi.org/10.3389/fphar.2014.00190 |
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author | Verheijden, Kim A. T. Henricks, Paul A. J. Redegeld, Frank A. Garssen, Johan Folkerts, Gert |
author_facet | Verheijden, Kim A. T. Henricks, Paul A. J. Redegeld, Frank A. Garssen, Johan Folkerts, Gert |
author_sort | Verheijden, Kim A. T. |
collection | PubMed |
description | In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (R(L)) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when R(L) was measured. Significant changes in both R(L) and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the R(L) measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring R(L) gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways. |
format | Online Article Text |
id | pubmed-4129625 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41296252014-08-26 Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice Verheijden, Kim A. T. Henricks, Paul A. J. Redegeld, Frank A. Garssen, Johan Folkerts, Gert Front Pharmacol Pharmacology In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (R(L)) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when R(L) was measured. Significant changes in both R(L) and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the R(L) measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring R(L) gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways. Frontiers Media S.A. 2014-08-12 /pmc/articles/PMC4129625/ /pubmed/25161620 http://dx.doi.org/10.3389/fphar.2014.00190 Text en Copyright © 2014 Verheijden, Henricks, Redegeld, Garssen and Folkerts. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Verheijden, Kim A. T. Henricks, Paul A. J. Redegeld, Frank A. Garssen, Johan Folkerts, Gert Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice |
title | Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice |
title_full | Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice |
title_fullStr | Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice |
title_full_unstemmed | Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice |
title_short | Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice |
title_sort | measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129625/ https://www.ncbi.nlm.nih.gov/pubmed/25161620 http://dx.doi.org/10.3389/fphar.2014.00190 |
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