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Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice

In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (R(L)) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inf...

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Autores principales: Verheijden, Kim A. T., Henricks, Paul A. J., Redegeld, Frank A., Garssen, Johan, Folkerts, Gert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129625/
https://www.ncbi.nlm.nih.gov/pubmed/25161620
http://dx.doi.org/10.3389/fphar.2014.00190
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author Verheijden, Kim A. T.
Henricks, Paul A. J.
Redegeld, Frank A.
Garssen, Johan
Folkerts, Gert
author_facet Verheijden, Kim A. T.
Henricks, Paul A. J.
Redegeld, Frank A.
Garssen, Johan
Folkerts, Gert
author_sort Verheijden, Kim A. T.
collection PubMed
description In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (R(L)) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when R(L) was measured. Significant changes in both R(L) and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the R(L) measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring R(L) gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways.
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spelling pubmed-41296252014-08-26 Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice Verheijden, Kim A. T. Henricks, Paul A. J. Redegeld, Frank A. Garssen, Johan Folkerts, Gert Front Pharmacol Pharmacology In this study a direct comparison was made between non-invasive and non-ventilated unrestrained whole body plethysmography (Penh) (conscious animals) and the invasive ventilated lung resistance (R(L)) method (anesthetized animals) in both mild and severe allergic airway inflammation models. Mild inflammation was induced by intraperitoneal sensitization and aerosols of ovalbumin. Severe inflammation was induced by intraperitoneal sensitization using trinitrophenyl-ovalbumin, followed by intranasal challenges with IgE-allergen complexes. A significant increase in airway responsiveness to methacholine was observed in the mild inflammation group when R(L) was measured. Significant changes in both R(L) and Penh were observed in the severe inflammation groups. There was a significant increase in the number of inflammatory cells in the Broncho-Alveolar Lavage Fluid (BALF) in both the mild and severe inflammation animals. The enforced ventilation of the animals during the R(L) measurement further increased the number of cells in the BALF. IL-2 and RANTES levels in the BALF were higher in the severe inflammation groups compared to the mild inflammation groups. Penh gave only reliable measurements during severe airway inflammation. Measuring R(L) gave consistent results in both mild and severe allergic airway inflammation models however, ventilation induced an additional cell influx into the airways. Frontiers Media S.A. 2014-08-12 /pmc/articles/PMC4129625/ /pubmed/25161620 http://dx.doi.org/10.3389/fphar.2014.00190 Text en Copyright © 2014 Verheijden, Henricks, Redegeld, Garssen and Folkerts. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Verheijden, Kim A. T.
Henricks, Paul A. J.
Redegeld, Frank A.
Garssen, Johan
Folkerts, Gert
Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
title Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
title_full Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
title_fullStr Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
title_full_unstemmed Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
title_short Measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
title_sort measurement of airway function using invasive and non-invasive methods in mild and severe models for allergic airway inflammation in mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4129625/
https://www.ncbi.nlm.nih.gov/pubmed/25161620
http://dx.doi.org/10.3389/fphar.2014.00190
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