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Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing
[Image: see text] Polymerized high internal phase emulsions (polyHIPEs) have been utilized in the creation of injectable scaffolds that cure in situ to fill irregular bone defects and potentially improve tissue healing. Previously, thermally initiated scaffolds required hours to cure, which diminish...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4130241/ https://www.ncbi.nlm.nih.gov/pubmed/25006990 http://dx.doi.org/10.1021/bm500754r |
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author | Moglia, Robert S. Whitely, Michael Dhavalikar, Prachi Robinson, Jennifer Pearce, Hannah Brooks, Megan Stuebben, Melissa Cordner, Nicole Cosgriff-Hernandez, Elizabeth |
author_facet | Moglia, Robert S. Whitely, Michael Dhavalikar, Prachi Robinson, Jennifer Pearce, Hannah Brooks, Megan Stuebben, Melissa Cordner, Nicole Cosgriff-Hernandez, Elizabeth |
author_sort | Moglia, Robert S. |
collection | PubMed |
description | [Image: see text] Polymerized high internal phase emulsions (polyHIPEs) have been utilized in the creation of injectable scaffolds that cure in situ to fill irregular bone defects and potentially improve tissue healing. Previously, thermally initiated scaffolds required hours to cure, which diminished the potential for clinical translation. Here, a double-barrel syringe system for fabricating redox-initiated polyHIPEs with dramatically shortened cure times upon injection was demonstrated with three methacrylated macromers. The polyHIPE cure time, compressive properties, and pore architecture were investigated with respect to redox initiator chemistry and concentration. Increased concentrations of redox initiators reduced cure times from hours to minutes and increased the compressive modulus and strength without compromising the pore architecture. Additionally, storage of the uncured emulsion at reduced temperatures for 6 months was shown to have minimal effects on the resulting graft properties. These studies indicate that the uncured emulsions can be stored in the clinic until they are needed and then rapidly cured after injection to rigid, high-porosity scaffolds. In summary, we have improved upon current methods of generating injectable polyHIPE grafts to meet translational design goals of long storage times and rapid curing (<15 min) without sacrificing porosity or mechanical properties. |
format | Online Article Text |
id | pubmed-4130241 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-41302412015-07-09 Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing Moglia, Robert S. Whitely, Michael Dhavalikar, Prachi Robinson, Jennifer Pearce, Hannah Brooks, Megan Stuebben, Melissa Cordner, Nicole Cosgriff-Hernandez, Elizabeth Biomacromolecules [Image: see text] Polymerized high internal phase emulsions (polyHIPEs) have been utilized in the creation of injectable scaffolds that cure in situ to fill irregular bone defects and potentially improve tissue healing. Previously, thermally initiated scaffolds required hours to cure, which diminished the potential for clinical translation. Here, a double-barrel syringe system for fabricating redox-initiated polyHIPEs with dramatically shortened cure times upon injection was demonstrated with three methacrylated macromers. The polyHIPE cure time, compressive properties, and pore architecture were investigated with respect to redox initiator chemistry and concentration. Increased concentrations of redox initiators reduced cure times from hours to minutes and increased the compressive modulus and strength without compromising the pore architecture. Additionally, storage of the uncured emulsion at reduced temperatures for 6 months was shown to have minimal effects on the resulting graft properties. These studies indicate that the uncured emulsions can be stored in the clinic until they are needed and then rapidly cured after injection to rigid, high-porosity scaffolds. In summary, we have improved upon current methods of generating injectable polyHIPE grafts to meet translational design goals of long storage times and rapid curing (<15 min) without sacrificing porosity or mechanical properties. American Chemical Society 2014-07-09 2014-08-11 /pmc/articles/PMC4130241/ /pubmed/25006990 http://dx.doi.org/10.1021/bm500754r Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
spellingShingle | Moglia, Robert S. Whitely, Michael Dhavalikar, Prachi Robinson, Jennifer Pearce, Hannah Brooks, Megan Stuebben, Melissa Cordner, Nicole Cosgriff-Hernandez, Elizabeth Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing |
title | Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing |
title_full | Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing |
title_fullStr | Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing |
title_full_unstemmed | Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing |
title_short | Injectable Polymerized High Internal Phase Emulsions with Rapid in Situ Curing |
title_sort | injectable polymerized high internal phase emulsions with rapid in situ curing |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4130241/ https://www.ncbi.nlm.nih.gov/pubmed/25006990 http://dx.doi.org/10.1021/bm500754r |
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