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An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice

Epidemiological evidence supports a protective effect of physical activity for breast cancer in older women, but the mechanisms are not well understood. We used 18-month-old BALB/c mice injected in the mammary fat pad with syngeneic 4T1 tumor cells as a model of invasive breast cancer. During the tu...

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Detalles Bibliográficos
Autores principales: Pettan-Brewer, Christina, Goh, Jorming, Ladiges, Warren C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131002/
https://www.ncbi.nlm.nih.gov/pubmed/25147645
http://dx.doi.org/10.3402/pba.v4.24824
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author Pettan-Brewer, Christina
Goh, Jorming
Ladiges, Warren C.
author_facet Pettan-Brewer, Christina
Goh, Jorming
Ladiges, Warren C.
author_sort Pettan-Brewer, Christina
collection PubMed
description Epidemiological evidence supports a protective effect of physical activity for breast cancer in older women, but the mechanisms are not well understood. We used 18-month-old BALB/c mice injected in the mammary fat pad with syngeneic 4T1 tumor cells as a model of invasive breast cancer. During the tumor progression phase, there was a significant decrease in labeling for F4/80, a marker for mouse macrophages, and CD34, a marker for vascular endothelial cells, in primary tumors from mice that ran higher average distances compared to mice that ran lower average distances (p≤0.05). These observations suggest that immunohistochemistry can be used to monitor stromal cell populations in tumors from old mice under exercise conditions.
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spelling pubmed-41310022014-08-21 An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice Pettan-Brewer, Christina Goh, Jorming Ladiges, Warren C. Pathobiol Aging Age Relat Dis Technical Report Epidemiological evidence supports a protective effect of physical activity for breast cancer in older women, but the mechanisms are not well understood. We used 18-month-old BALB/c mice injected in the mammary fat pad with syngeneic 4T1 tumor cells as a model of invasive breast cancer. During the tumor progression phase, there was a significant decrease in labeling for F4/80, a marker for mouse macrophages, and CD34, a marker for vascular endothelial cells, in primary tumors from mice that ran higher average distances compared to mice that ran lower average distances (p≤0.05). These observations suggest that immunohistochemistry can be used to monitor stromal cell populations in tumors from old mice under exercise conditions. Co-Action Publishing 2014-08-11 /pmc/articles/PMC4131002/ /pubmed/25147645 http://dx.doi.org/10.3402/pba.v4.24824 Text en © 2014 Christina Pettan-Brewer et al. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Report
Pettan-Brewer, Christina
Goh, Jorming
Ladiges, Warren C.
An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice
title An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice
title_full An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice
title_fullStr An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice
title_full_unstemmed An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice
title_short An immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice
title_sort immunohistochemical approach for monitoring effects of exercise on tumor stromal cells in old mice
topic Technical Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131002/
https://www.ncbi.nlm.nih.gov/pubmed/25147645
http://dx.doi.org/10.3402/pba.v4.24824
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