Cargando…
Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna
The Cerrado is a biome that corresponds to 24% of Brazil's territory. Only recently microbial communities of this biome have been investigated. Here we describe for the first time the diversity of archaeal communities from freshwater lake sediments of the Cerrado in the dry season and in the tr...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131120/ https://www.ncbi.nlm.nih.gov/pubmed/25147480 http://dx.doi.org/10.1155/2014/957145 |
_version_ | 1782330414807908352 |
---|---|
author | Rodrigues, Thiago Catão, Elisa Bustamante, Mercedes M. C. Quirino, Betania F. Kruger, Ricardo H. Kyaw, Cynthia M. |
author_facet | Rodrigues, Thiago Catão, Elisa Bustamante, Mercedes M. C. Quirino, Betania F. Kruger, Ricardo H. Kyaw, Cynthia M. |
author_sort | Rodrigues, Thiago |
collection | PubMed |
description | The Cerrado is a biome that corresponds to 24% of Brazil's territory. Only recently microbial communities of this biome have been investigated. Here we describe for the first time the diversity of archaeal communities from freshwater lake sediments of the Cerrado in the dry season and in the transition period between the dry and rainy seasons, when the first rains occur. Gene libraries were constructed, using Archaea-specific primers for the 16S rRNA and amoA genes. Analysis revealed marked differences between the archaeal communities found in the two seasons. I.1a and I.1c Thaumarchaeota were found in greater numbers in the transition period, while MCG Archaea was dominant on the dry season. Methanogens were only found in the dry season. Analysis of 16S rRNA sequences revealed lower diversity on the transition period. We detected archaeal amoA sequences in both seasons, but there were more OTUs during the dry season. These sequences were within the same cluster as Nitrosotalea devanaterra's amoA gene. The principal coordinate analysis (PCoA) test revealed significant differences between samples from different seasons. These results provide information on archaeal diversity in freshwater lake sediments of the Cerrado and indicates that rain is likely a factor that impacts these communities. |
format | Online Article Text |
id | pubmed-4131120 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41311202014-08-21 Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna Rodrigues, Thiago Catão, Elisa Bustamante, Mercedes M. C. Quirino, Betania F. Kruger, Ricardo H. Kyaw, Cynthia M. Archaea Research Article The Cerrado is a biome that corresponds to 24% of Brazil's territory. Only recently microbial communities of this biome have been investigated. Here we describe for the first time the diversity of archaeal communities from freshwater lake sediments of the Cerrado in the dry season and in the transition period between the dry and rainy seasons, when the first rains occur. Gene libraries were constructed, using Archaea-specific primers for the 16S rRNA and amoA genes. Analysis revealed marked differences between the archaeal communities found in the two seasons. I.1a and I.1c Thaumarchaeota were found in greater numbers in the transition period, while MCG Archaea was dominant on the dry season. Methanogens were only found in the dry season. Analysis of 16S rRNA sequences revealed lower diversity on the transition period. We detected archaeal amoA sequences in both seasons, but there were more OTUs during the dry season. These sequences were within the same cluster as Nitrosotalea devanaterra's amoA gene. The principal coordinate analysis (PCoA) test revealed significant differences between samples from different seasons. These results provide information on archaeal diversity in freshwater lake sediments of the Cerrado and indicates that rain is likely a factor that impacts these communities. Hindawi Publishing Corporation 2014-07-20 /pmc/articles/PMC4131120/ /pubmed/25147480 http://dx.doi.org/10.1155/2014/957145 Text en Copyright © 2014 Thiago Rodrigues et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Rodrigues, Thiago Catão, Elisa Bustamante, Mercedes M. C. Quirino, Betania F. Kruger, Ricardo H. Kyaw, Cynthia M. Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna |
title | Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna |
title_full | Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna |
title_fullStr | Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna |
title_full_unstemmed | Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna |
title_short | Seasonal Effects in a Lake Sediment Archaeal Community of the Brazilian Savanna |
title_sort | seasonal effects in a lake sediment archaeal community of the brazilian savanna |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131120/ https://www.ncbi.nlm.nih.gov/pubmed/25147480 http://dx.doi.org/10.1155/2014/957145 |
work_keys_str_mv | AT rodriguesthiago seasonaleffectsinalakesedimentarchaealcommunityofthebraziliansavanna AT cataoelisa seasonaleffectsinalakesedimentarchaealcommunityofthebraziliansavanna AT bustamantemercedesmc seasonaleffectsinalakesedimentarchaealcommunityofthebraziliansavanna AT quirinobetaniaf seasonaleffectsinalakesedimentarchaealcommunityofthebraziliansavanna AT krugerricardoh seasonaleffectsinalakesedimentarchaealcommunityofthebraziliansavanna AT kyawcynthiam seasonaleffectsinalakesedimentarchaealcommunityofthebraziliansavanna |