Cargando…
The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice
We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separa...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131508/ https://www.ncbi.nlm.nih.gov/pubmed/25147807 http://dx.doi.org/10.1155/2014/572949 |
_version_ | 1782330472461762560 |
---|---|
author | Cavalcante Miranda de Assis, Diana Martins Lima, Êmyle Teixeira Goes, Bruno Zugaib Cavalcanti, João Barbosa Paixão, Alaí Vannier-Santos, Marcos André Martinez, Ana Maria Blanco Baptista, Abrahão Fontes |
author_facet | Cavalcante Miranda de Assis, Diana Martins Lima, Êmyle Teixeira Goes, Bruno Zugaib Cavalcanti, João Barbosa Paixão, Alaí Vannier-Santos, Marcos André Martinez, Ana Maria Blanco Baptista, Abrahão Fontes |
author_sort | Cavalcante Miranda de Assis, Diana |
collection | PubMed |
description | We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separated into three groups: nontreated, Low-TENS (4 Hz), and High-TENS (100 Hz). The animals of Low- and High-TENS groups were stimulated for 2 h immediately after the surgical procedure, while the nontreated group was only positioned for the same period. After five weeks the animals were euthanized, and the nerves dissected bilaterally for histological and histomorphometric analysis. Histological assessment by light and electron microscopy showed that High-TENS and nontreated nerves had a similar profile, with extensive signs of degeneration. Conversely, Low-TENS led to increased regeneration, displaying histological aspects similar to control nerves. High-TENS also led to decreased density of fibers in the range of 6–12 μm diameter and decreased fiber diameter and myelin area in the range of 0–2 μm diameter. These findings suggest that High-TENS applied just after a peripheral nerve crush may be deleterious for regeneration, whereas Low-TENS may increase nerve regeneration capacity. |
format | Online Article Text |
id | pubmed-4131508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41315082014-08-21 The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice Cavalcante Miranda de Assis, Diana Martins Lima, Êmyle Teixeira Goes, Bruno Zugaib Cavalcanti, João Barbosa Paixão, Alaí Vannier-Santos, Marcos André Martinez, Ana Maria Blanco Baptista, Abrahão Fontes Biomed Res Int Research Article We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separated into three groups: nontreated, Low-TENS (4 Hz), and High-TENS (100 Hz). The animals of Low- and High-TENS groups were stimulated for 2 h immediately after the surgical procedure, while the nontreated group was only positioned for the same period. After five weeks the animals were euthanized, and the nerves dissected bilaterally for histological and histomorphometric analysis. Histological assessment by light and electron microscopy showed that High-TENS and nontreated nerves had a similar profile, with extensive signs of degeneration. Conversely, Low-TENS led to increased regeneration, displaying histological aspects similar to control nerves. High-TENS also led to decreased density of fibers in the range of 6–12 μm diameter and decreased fiber diameter and myelin area in the range of 0–2 μm diameter. These findings suggest that High-TENS applied just after a peripheral nerve crush may be deleterious for regeneration, whereas Low-TENS may increase nerve regeneration capacity. Hindawi Publishing Corporation 2014 2014-07-24 /pmc/articles/PMC4131508/ /pubmed/25147807 http://dx.doi.org/10.1155/2014/572949 Text en Copyright © 2014 Diana Cavalcante Miranda de Assis et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cavalcante Miranda de Assis, Diana Martins Lima, Êmyle Teixeira Goes, Bruno Zugaib Cavalcanti, João Barbosa Paixão, Alaí Vannier-Santos, Marcos André Martinez, Ana Maria Blanco Baptista, Abrahão Fontes The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice |
title | The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice |
title_full | The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice |
title_fullStr | The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice |
title_full_unstemmed | The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice |
title_short | The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice |
title_sort | parameters of transcutaneous electrical nerve stimulation are critical to its regenerative effects when applied just after a sciatic crush lesion in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131508/ https://www.ncbi.nlm.nih.gov/pubmed/25147807 http://dx.doi.org/10.1155/2014/572949 |
work_keys_str_mv | AT cavalcantemirandadeassisdiana theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT martinslimaemyle theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT teixeiragoesbruno theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT zugaibcavalcantijoao theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT barbosapaixaoalai theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT vanniersantosmarcosandre theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT martinezanamariablanco theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT baptistaabrahaofontes theparametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT cavalcantemirandadeassisdiana parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT martinslimaemyle parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT teixeiragoesbruno parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT zugaibcavalcantijoao parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT barbosapaixaoalai parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT vanniersantosmarcosandre parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT martinezanamariablanco parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice AT baptistaabrahaofontes parametersoftranscutaneouselectricalnervestimulationarecriticaltoitsregenerativeeffectswhenappliedjustafterasciaticcrushlesioninmice |