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The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice

We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separa...

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Autores principales: Cavalcante Miranda de Assis, Diana, Martins Lima, Êmyle, Teixeira Goes, Bruno, Zugaib Cavalcanti, João, Barbosa Paixão, Alaí, Vannier-Santos, Marcos André, Martinez, Ana Maria Blanco, Baptista, Abrahão Fontes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131508/
https://www.ncbi.nlm.nih.gov/pubmed/25147807
http://dx.doi.org/10.1155/2014/572949
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author Cavalcante Miranda de Assis, Diana
Martins Lima, Êmyle
Teixeira Goes, Bruno
Zugaib Cavalcanti, João
Barbosa Paixão, Alaí
Vannier-Santos, Marcos André
Martinez, Ana Maria Blanco
Baptista, Abrahão Fontes
author_facet Cavalcante Miranda de Assis, Diana
Martins Lima, Êmyle
Teixeira Goes, Bruno
Zugaib Cavalcanti, João
Barbosa Paixão, Alaí
Vannier-Santos, Marcos André
Martinez, Ana Maria Blanco
Baptista, Abrahão Fontes
author_sort Cavalcante Miranda de Assis, Diana
collection PubMed
description We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separated into three groups: nontreated, Low-TENS (4 Hz), and High-TENS (100 Hz). The animals of Low- and High-TENS groups were stimulated for 2 h immediately after the surgical procedure, while the nontreated group was only positioned for the same period. After five weeks the animals were euthanized, and the nerves dissected bilaterally for histological and histomorphometric analysis. Histological assessment by light and electron microscopy showed that High-TENS and nontreated nerves had a similar profile, with extensive signs of degeneration. Conversely, Low-TENS led to increased regeneration, displaying histological aspects similar to control nerves. High-TENS also led to decreased density of fibers in the range of 6–12 μm diameter and decreased fiber diameter and myelin area in the range of 0–2 μm diameter. These findings suggest that High-TENS applied just after a peripheral nerve crush may be deleterious for regeneration, whereas Low-TENS may increase nerve regeneration capacity.
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spelling pubmed-41315082014-08-21 The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice Cavalcante Miranda de Assis, Diana Martins Lima, Êmyle Teixeira Goes, Bruno Zugaib Cavalcanti, João Barbosa Paixão, Alaí Vannier-Santos, Marcos André Martinez, Ana Maria Blanco Baptista, Abrahão Fontes Biomed Res Int Research Article We investigated the effect of two frequencies of transcutaneous electrical nerve stimulation (TENS) applied immediately after lesion on peripheral nerve regeneration after a mouse sciatic crush injury. The animals were anesthetized and subjected to crushing of the right sciatic nerve and then separated into three groups: nontreated, Low-TENS (4 Hz), and High-TENS (100 Hz). The animals of Low- and High-TENS groups were stimulated for 2 h immediately after the surgical procedure, while the nontreated group was only positioned for the same period. After five weeks the animals were euthanized, and the nerves dissected bilaterally for histological and histomorphometric analysis. Histological assessment by light and electron microscopy showed that High-TENS and nontreated nerves had a similar profile, with extensive signs of degeneration. Conversely, Low-TENS led to increased regeneration, displaying histological aspects similar to control nerves. High-TENS also led to decreased density of fibers in the range of 6–12 μm diameter and decreased fiber diameter and myelin area in the range of 0–2 μm diameter. These findings suggest that High-TENS applied just after a peripheral nerve crush may be deleterious for regeneration, whereas Low-TENS may increase nerve regeneration capacity. Hindawi Publishing Corporation 2014 2014-07-24 /pmc/articles/PMC4131508/ /pubmed/25147807 http://dx.doi.org/10.1155/2014/572949 Text en Copyright © 2014 Diana Cavalcante Miranda de Assis et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cavalcante Miranda de Assis, Diana
Martins Lima, Êmyle
Teixeira Goes, Bruno
Zugaib Cavalcanti, João
Barbosa Paixão, Alaí
Vannier-Santos, Marcos André
Martinez, Ana Maria Blanco
Baptista, Abrahão Fontes
The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice
title The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice
title_full The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice
title_fullStr The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice
title_full_unstemmed The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice
title_short The Parameters of Transcutaneous Electrical Nerve Stimulation Are Critical to Its Regenerative Effects When Applied Just after a Sciatic Crush Lesion in Mice
title_sort parameters of transcutaneous electrical nerve stimulation are critical to its regenerative effects when applied just after a sciatic crush lesion in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131508/
https://www.ncbi.nlm.nih.gov/pubmed/25147807
http://dx.doi.org/10.1155/2014/572949
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