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Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus

Multiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicr...

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Autores principales: Huang, Jonathan P., Mojib, Nazia, Goli, Rakesh R., Watkins, Samantha, Waites, Ken B., Ravindra, Rasik, Andersen, Dale T., Bej, Asim K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131597/
http://dx.doi.org/10.1007/s13659-012-0021-4
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author Huang, Jonathan P.
Mojib, Nazia
Goli, Rakesh R.
Watkins, Samantha
Waites, Ken B.
Ravindra, Rasik
Andersen, Dale T.
Bej, Asim K.
author_facet Huang, Jonathan P.
Mojib, Nazia
Goli, Rakesh R.
Watkins, Samantha
Waites, Ken B.
Ravindra, Rasik
Andersen, Dale T.
Bej, Asim K.
author_sort Huang, Jonathan P.
collection PubMed
description Multiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen. In this study, we have investigated the antimicrobial activity of a purple violet pigment (PVP) from an Antarctic bacterium, Janthinobacterium sp. Ant5-2 on 15 clinical MDR and MRSA strains. The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration (MIC(90)) of PVP against MDR and MRSA. The MIC(90) ranged between 1.57 µg/mL and 3.13 µg/mL, which are significantly lower than many antimicrobials tested from natural sources against this pathogen. The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead® BacLight™ fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels. In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound. The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens. [Image: see text]
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spelling pubmed-41315972014-08-20 Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus Huang, Jonathan P. Mojib, Nazia Goli, Rakesh R. Watkins, Samantha Waites, Ken B. Ravindra, Rasik Andersen, Dale T. Bej, Asim K. Nat Prod Bioprospect Regular Article Multiple drug resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) have become increasingly prevalent as a community acquired infection. As a result limited treatment options are available with conventional synthetic antibiotics. Bioprospecting natural products with potent antimicrobial activity show promise for developing new drugs against this pathogen. In this study, we have investigated the antimicrobial activity of a purple violet pigment (PVP) from an Antarctic bacterium, Janthinobacterium sp. Ant5-2 on 15 clinical MDR and MRSA strains. The colorimetric resazurin assay was employed to determine the minimum inhibitory concentration (MIC(90)) of PVP against MDR and MRSA. The MIC(90) ranged between 1.57 µg/mL and 3.13 µg/mL, which are significantly lower than many antimicrobials tested from natural sources against this pathogen. The spectrophotometrically determined growth analysis and total microscopic counts using Live/dead® BacLight™ fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC levels. In silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial compound. The continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens. [Image: see text] Springer Berlin Heidelberg 2012-04-11 /pmc/articles/PMC4131597/ http://dx.doi.org/10.1007/s13659-012-0021-4 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/This article is published under license to BioMed Central Ltd. Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Regular Article
Huang, Jonathan P.
Mojib, Nazia
Goli, Rakesh R.
Watkins, Samantha
Waites, Ken B.
Ravindra, Rasik
Andersen, Dale T.
Bej, Asim K.
Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
title Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
title_full Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
title_fullStr Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
title_full_unstemmed Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
title_short Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus
title_sort antimicrobial activity of pvp from an antarctic bacterium, janthinobacterium sp. ant5-2, on multi-drug and methicillin resistant staphylococcus aureus
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131597/
http://dx.doi.org/10.1007/s13659-012-0021-4
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