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Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients

Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions. In the current study, self and clinician-collected vaginal and cervical samples from women were processed to detect HPV DNA using polymerase chain reaction (PCR) with PGMY09/11 primers. HPV genotypes...

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Autores principales: de Campos, Karla Lopes Mandu, Machado, Ana Paula, de Almeida, Flávia Gatto, Bonin, Camila Mareti, Prata, Thiago Theodoro Martins, Almeida, Larissa Zatorre, Padovani, Cacilda Tezelli Junqueira, Ferreira, Alda Maria Teixeira, Fernandes, Carlos Eurico dos Santos, Tozetti, Inês Aparecida
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131789/
https://www.ncbi.nlm.nih.gov/pubmed/24831549
http://dx.doi.org/10.1590/0074-0276130397
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author de Campos, Karla Lopes Mandu
Machado, Ana Paula
de Almeida, Flávia Gatto
Bonin, Camila Mareti
Prata, Thiago Theodoro Martins
Almeida, Larissa Zatorre
Padovani, Cacilda Tezelli Junqueira
Ferreira, Alda Maria Teixeira
Fernandes, Carlos Eurico dos Santos
Tozetti, Inês Aparecida
author_facet de Campos, Karla Lopes Mandu
Machado, Ana Paula
de Almeida, Flávia Gatto
Bonin, Camila Mareti
Prata, Thiago Theodoro Martins
Almeida, Larissa Zatorre
Padovani, Cacilda Tezelli Junqueira
Ferreira, Alda Maria Teixeira
Fernandes, Carlos Eurico dos Santos
Tozetti, Inês Aparecida
author_sort de Campos, Karla Lopes Mandu
collection PubMed
description Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions. In the current study, self and clinician-collected vaginal and cervical samples from women were processed to detect HPV DNA using polymerase chain reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using type-specific PCR. HPV DNA detection showed good concordance between self and clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30% women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV was detected at a higher frequency in self-collected; however, HR-HPV types were more frequently identified in clinician-collected samples than in self-collected samples. HPV infections of multiple types were detected in 20.5% of clinician-collected samples and 15.5% of self-collected samples. In this study, we demonstrated that the HPV DNA detection rate in self-collected samples has good agreement with that of clinician-collected samples. Self-collected sampling, as a primary prevention strategy in countries with few resources, could be effective for identifying cases of HR-HPV, being more acceptable. The use of this method would enhance the coverage of screening programs for cervical cancer.
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spelling pubmed-41317892014-08-14 Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients de Campos, Karla Lopes Mandu Machado, Ana Paula de Almeida, Flávia Gatto Bonin, Camila Mareti Prata, Thiago Theodoro Martins Almeida, Larissa Zatorre Padovani, Cacilda Tezelli Junqueira Ferreira, Alda Maria Teixeira Fernandes, Carlos Eurico dos Santos Tozetti, Inês Aparecida Mem Inst Oswaldo Cruz Articles Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions. In the current study, self and clinician-collected vaginal and cervical samples from women were processed to detect HPV DNA using polymerase chain reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using type-specific PCR. HPV DNA detection showed good concordance between self and clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30% women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV was detected at a higher frequency in self-collected; however, HR-HPV types were more frequently identified in clinician-collected samples than in self-collected samples. HPV infections of multiple types were detected in 20.5% of clinician-collected samples and 15.5% of self-collected samples. In this study, we demonstrated that the HPV DNA detection rate in self-collected samples has good agreement with that of clinician-collected samples. Self-collected sampling, as a primary prevention strategy in countries with few resources, could be effective for identifying cases of HR-HPV, being more acceptable. The use of this method would enhance the coverage of screening programs for cervical cancer. Instituto Oswaldo Cruz, Ministério da Saúde 2014-04-25 2014-06 /pmc/articles/PMC4131789/ /pubmed/24831549 http://dx.doi.org/10.1590/0074-0276130397 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
de Campos, Karla Lopes Mandu
Machado, Ana Paula
de Almeida, Flávia Gatto
Bonin, Camila Mareti
Prata, Thiago Theodoro Martins
Almeida, Larissa Zatorre
Padovani, Cacilda Tezelli Junqueira
Ferreira, Alda Maria Teixeira
Fernandes, Carlos Eurico dos Santos
Tozetti, Inês Aparecida
Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients
title Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients
title_full Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients
title_fullStr Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients
title_full_unstemmed Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients
title_short Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients
title_sort good agreements between self and clinician-collected specimens for the detection of human papillomavirus in brazilian patients
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131789/
https://www.ncbi.nlm.nih.gov/pubmed/24831549
http://dx.doi.org/10.1590/0074-0276130397
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