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Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres
Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro. However their adoption in regular screening programs has been hindered by uneven culture growth, poor reproducibility and...
Autores principales: | Ivanov, Delyan P., Parker, Terry L., Walker, David A., Alexander, Cameron, Ashford, Marianne B., Gellert, Paul R., Garnett, Martin C. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4131917/ https://www.ncbi.nlm.nih.gov/pubmed/25119185 http://dx.doi.org/10.1371/journal.pone.0103817 |
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