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Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials

OBJECTIVES: To investigate the effect of seasonal variation on adult clinical laboratory parameters in Rwanda, Zambia, and Uganda and determine its implications for HIV prevention and other clinical trials. METHODS: Volunteers in a cross-sectional study to establish laboratory reference intervals we...

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Autores principales: Ruzagira, Eugene, Abaasa, Andrew, Karita, Etienne, Mulenga, Joseph, Kilembe, William, Allen, Susan, Bahemuka, Ubaldo, Bwanika, Agnes N., Levin, Jonathan, Price, Matthew A., Kamali, Anatoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132051/
https://www.ncbi.nlm.nih.gov/pubmed/25118593
http://dx.doi.org/10.1371/journal.pone.0105089
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author Ruzagira, Eugene
Abaasa, Andrew
Karita, Etienne
Mulenga, Joseph
Kilembe, William
Allen, Susan
Bahemuka, Ubaldo
Bwanika, Agnes N.
Levin, Jonathan
Price, Matthew A.
Kamali, Anatoli
author_facet Ruzagira, Eugene
Abaasa, Andrew
Karita, Etienne
Mulenga, Joseph
Kilembe, William
Allen, Susan
Bahemuka, Ubaldo
Bwanika, Agnes N.
Levin, Jonathan
Price, Matthew A.
Kamali, Anatoli
author_sort Ruzagira, Eugene
collection PubMed
description OBJECTIVES: To investigate the effect of seasonal variation on adult clinical laboratory parameters in Rwanda, Zambia, and Uganda and determine its implications for HIV prevention and other clinical trials. METHODS: Volunteers in a cross-sectional study to establish laboratory reference intervals were asked to return for a seasonal visit after the local season had changed from dry to rainy or vice versa. Volunteers had to be clinically healthy, not pregnant and negative for HIV, Hepatitis B and C, and syphilis infection at both visits. At each visit, blood was taken for measurement of hemoglobin, haematocrit, mean corpuscular volume, red blood cells, platelets, total white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, basophils, CD4/CD8 T cells, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, direct bilirubin, total bilirubin, total immunoglobulin gamma, total protein, creatinine, total amylase, creatine phosphokinase and lactate dehydrogenase (LDH). Consensus dry season reference intervals were applied to rainy season values (and vice versa) and the proportion of ‘out-of-range’ values determined. Percentage differences between dry and rainy season parameter mean values were estimated. RESULTS: In this cohort of 903 volunteers, less than 10.0% of consensus parameter (except LDH) values in one season were “out-of-range” in the other. Twenty-two (22) percent of rainy season LDH values fell outside of the consensus dry season interval with the higher values observed in the rainy season. Variability between consensus seasonal means ranged from 0.0% (total WBC, neutrophils, monocytes, basophils, and direct bilirubin) to 40.0% (eosinophils). Within sites, the largest seasonal variations were observed for monocytes (Masaka, 11.5%), LDH (Lusaka, 21.7%), and basophils (Kigali, 22.2%). CONCLUSIONS: Seasonality had minimal impact on adult clinical laboratory parameter values in Rwanda, Zambia, and Uganda. Seasonal variation may not be an important factor in the evaluation of adult clinical laboratory parameters in HIV prevention and other clinical trials in these countries.
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spelling pubmed-41320512014-08-19 Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials Ruzagira, Eugene Abaasa, Andrew Karita, Etienne Mulenga, Joseph Kilembe, William Allen, Susan Bahemuka, Ubaldo Bwanika, Agnes N. Levin, Jonathan Price, Matthew A. Kamali, Anatoli PLoS One Research Article OBJECTIVES: To investigate the effect of seasonal variation on adult clinical laboratory parameters in Rwanda, Zambia, and Uganda and determine its implications for HIV prevention and other clinical trials. METHODS: Volunteers in a cross-sectional study to establish laboratory reference intervals were asked to return for a seasonal visit after the local season had changed from dry to rainy or vice versa. Volunteers had to be clinically healthy, not pregnant and negative for HIV, Hepatitis B and C, and syphilis infection at both visits. At each visit, blood was taken for measurement of hemoglobin, haematocrit, mean corpuscular volume, red blood cells, platelets, total white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, basophils, CD4/CD8 T cells, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, direct bilirubin, total bilirubin, total immunoglobulin gamma, total protein, creatinine, total amylase, creatine phosphokinase and lactate dehydrogenase (LDH). Consensus dry season reference intervals were applied to rainy season values (and vice versa) and the proportion of ‘out-of-range’ values determined. Percentage differences between dry and rainy season parameter mean values were estimated. RESULTS: In this cohort of 903 volunteers, less than 10.0% of consensus parameter (except LDH) values in one season were “out-of-range” in the other. Twenty-two (22) percent of rainy season LDH values fell outside of the consensus dry season interval with the higher values observed in the rainy season. Variability between consensus seasonal means ranged from 0.0% (total WBC, neutrophils, monocytes, basophils, and direct bilirubin) to 40.0% (eosinophils). Within sites, the largest seasonal variations were observed for monocytes (Masaka, 11.5%), LDH (Lusaka, 21.7%), and basophils (Kigali, 22.2%). CONCLUSIONS: Seasonality had minimal impact on adult clinical laboratory parameter values in Rwanda, Zambia, and Uganda. Seasonal variation may not be an important factor in the evaluation of adult clinical laboratory parameters in HIV prevention and other clinical trials in these countries. Public Library of Science 2014-08-13 /pmc/articles/PMC4132051/ /pubmed/25118593 http://dx.doi.org/10.1371/journal.pone.0105089 Text en © 2014 Ruzagira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ruzagira, Eugene
Abaasa, Andrew
Karita, Etienne
Mulenga, Joseph
Kilembe, William
Allen, Susan
Bahemuka, Ubaldo
Bwanika, Agnes N.
Levin, Jonathan
Price, Matthew A.
Kamali, Anatoli
Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials
title Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials
title_full Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials
title_fullStr Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials
title_full_unstemmed Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials
title_short Effect of Seasonal Variation on Adult Clinical Laboratory Parameters in Rwanda, Zambia, and Uganda: Implications for HIV Biomedical Prevention Trials
title_sort effect of seasonal variation on adult clinical laboratory parameters in rwanda, zambia, and uganda: implications for hiv biomedical prevention trials
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132051/
https://www.ncbi.nlm.nih.gov/pubmed/25118593
http://dx.doi.org/10.1371/journal.pone.0105089
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