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DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin
The regulation of chromatin structure in eukaryotic cells involves abundant architectural factors such as high mobility group B (HMGB) proteins. It is not understood how these factors control the interplay between genome accessibility and compaction. In vivo, HMO1 binds the promoter and coding regio...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132745/ https://www.ncbi.nlm.nih.gov/pubmed/25063301 http://dx.doi.org/10.1093/nar/gku635 |
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author | Murugesapillai, Divakaran McCauley, Micah J. Huo, Ran Nelson Holte, Molly H. Stepanyants, Armen Maher, L. James Israeloff, Nathan E. Williams, Mark C. |
author_facet | Murugesapillai, Divakaran McCauley, Micah J. Huo, Ran Nelson Holte, Molly H. Stepanyants, Armen Maher, L. James Israeloff, Nathan E. Williams, Mark C. |
author_sort | Murugesapillai, Divakaran |
collection | PubMed |
description | The regulation of chromatin structure in eukaryotic cells involves abundant architectural factors such as high mobility group B (HMGB) proteins. It is not understood how these factors control the interplay between genome accessibility and compaction. In vivo, HMO1 binds the promoter and coding regions of most ribosomal RNA genes, facilitating transcription and possibly stabilizing chromatin in the absence of histones. To understand how HMO1 performs these functions, we combine single molecule stretching and atomic force microscopy (AFM). By stretching HMO1-bound DNA, we demonstrate a hierarchical organization of interactions, in which HMO1 initially compacts DNA on a timescale of seconds, followed by bridge formation and stabilization of DNA loops on a timescale of minutes. AFM experiments demonstrate DNA bridging between strands as well as looping by HMO1. Our results support a model in which HMO1 maintains the stability of nucleosome-free chromatin regions by forming complex and dynamic DNA structures mediated by protein–protein interactions. |
format | Online Article Text |
id | pubmed-4132745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41327452014-12-01 DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin Murugesapillai, Divakaran McCauley, Micah J. Huo, Ran Nelson Holte, Molly H. Stepanyants, Armen Maher, L. James Israeloff, Nathan E. Williams, Mark C. Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The regulation of chromatin structure in eukaryotic cells involves abundant architectural factors such as high mobility group B (HMGB) proteins. It is not understood how these factors control the interplay between genome accessibility and compaction. In vivo, HMO1 binds the promoter and coding regions of most ribosomal RNA genes, facilitating transcription and possibly stabilizing chromatin in the absence of histones. To understand how HMO1 performs these functions, we combine single molecule stretching and atomic force microscopy (AFM). By stretching HMO1-bound DNA, we demonstrate a hierarchical organization of interactions, in which HMO1 initially compacts DNA on a timescale of seconds, followed by bridge formation and stabilization of DNA loops on a timescale of minutes. AFM experiments demonstrate DNA bridging between strands as well as looping by HMO1. Our results support a model in which HMO1 maintains the stability of nucleosome-free chromatin regions by forming complex and dynamic DNA structures mediated by protein–protein interactions. Oxford University Press 2014-08-18 2014-07-24 /pmc/articles/PMC4132745/ /pubmed/25063301 http://dx.doi.org/10.1093/nar/gku635 Text en © The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene regulation, Chromatin and Epigenetics Murugesapillai, Divakaran McCauley, Micah J. Huo, Ran Nelson Holte, Molly H. Stepanyants, Armen Maher, L. James Israeloff, Nathan E. Williams, Mark C. DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin |
title | DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin |
title_full | DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin |
title_fullStr | DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin |
title_full_unstemmed | DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin |
title_short | DNA bridging and looping by HMO1 provides a mechanism for stabilizing nucleosome-free chromatin |
title_sort | dna bridging and looping by hmo1 provides a mechanism for stabilizing nucleosome-free chromatin |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132745/ https://www.ncbi.nlm.nih.gov/pubmed/25063301 http://dx.doi.org/10.1093/nar/gku635 |
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