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Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells

BACKGROUND: In Europe, extracts of Equisetum arvense (common horsetail) have a long tradition in the treatment of inflammatory disorders. To understand the molecular basis for its use, we investigated the immunomodulatory capacity of a standardized commercially available common horsetail extract on...

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Autores principales: Gründemann, Carsten, Lengen, Karin, Sauer, Barbara, Garcia-Käufer, Manuel, Zehl, Martin, Huber, Roman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132922/
https://www.ncbi.nlm.nih.gov/pubmed/25088216
http://dx.doi.org/10.1186/1472-6882-14-283
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author Gründemann, Carsten
Lengen, Karin
Sauer, Barbara
Garcia-Käufer, Manuel
Zehl, Martin
Huber, Roman
author_facet Gründemann, Carsten
Lengen, Karin
Sauer, Barbara
Garcia-Käufer, Manuel
Zehl, Martin
Huber, Roman
author_sort Gründemann, Carsten
collection PubMed
description BACKGROUND: In Europe, extracts of Equisetum arvense (common horsetail) have a long tradition in the treatment of inflammatory disorders. To understand the molecular basis for its use, we investigated the immunomodulatory capacity of a standardized commercially available common horsetail extract on human primary lymphocyte function in vitro. METHODS: The standardized extract of Equisetum arvense was phytochemically characterized. Effects on proliferation, viability and activity of mitogen-activated human lymphocytes were assessed in comparison to cyclosporine A using annexin V/propidium iodide staining assays and flow cytometry-based surface receptor characterization, respectively. Intracellular levels of effector molecules (IL-2, IFN-γ and TNF-α) were analyzed with cytokine assays. RESULTS: T cell proliferation was inhibited dose dependently by the Equisetum extract without induction of apoptosis or necrosis. This effect was mediated through inhibition of lymphocyte activation, specifically by diminishing CD69 and IL-2 surface receptor expression and intracellular IL-2 production. Furthermore, treatment with Equisetum arvense inhibited effector functions, as indicated by reduced production of IFN-γ and TNF-α. CONCLUSIONS: The data indicate that the used extract of Equisetum arvense interferes with the polyfunctionality of immunocompetent cells thereby providing an anti-inflammatory mode-of-action. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1472-6882-14-283) contains supplementary material, which is available to authorized users.
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spelling pubmed-41329222014-08-15 Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells Gründemann, Carsten Lengen, Karin Sauer, Barbara Garcia-Käufer, Manuel Zehl, Martin Huber, Roman BMC Complement Altern Med Research Article BACKGROUND: In Europe, extracts of Equisetum arvense (common horsetail) have a long tradition in the treatment of inflammatory disorders. To understand the molecular basis for its use, we investigated the immunomodulatory capacity of a standardized commercially available common horsetail extract on human primary lymphocyte function in vitro. METHODS: The standardized extract of Equisetum arvense was phytochemically characterized. Effects on proliferation, viability and activity of mitogen-activated human lymphocytes were assessed in comparison to cyclosporine A using annexin V/propidium iodide staining assays and flow cytometry-based surface receptor characterization, respectively. Intracellular levels of effector molecules (IL-2, IFN-γ and TNF-α) were analyzed with cytokine assays. RESULTS: T cell proliferation was inhibited dose dependently by the Equisetum extract without induction of apoptosis or necrosis. This effect was mediated through inhibition of lymphocyte activation, specifically by diminishing CD69 and IL-2 surface receptor expression and intracellular IL-2 production. Furthermore, treatment with Equisetum arvense inhibited effector functions, as indicated by reduced production of IFN-γ and TNF-α. CONCLUSIONS: The data indicate that the used extract of Equisetum arvense interferes with the polyfunctionality of immunocompetent cells thereby providing an anti-inflammatory mode-of-action. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1472-6882-14-283) contains supplementary material, which is available to authorized users. BioMed Central 2014-08-04 /pmc/articles/PMC4132922/ /pubmed/25088216 http://dx.doi.org/10.1186/1472-6882-14-283 Text en © Gründemann et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Gründemann, Carsten
Lengen, Karin
Sauer, Barbara
Garcia-Käufer, Manuel
Zehl, Martin
Huber, Roman
Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells
title Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells
title_full Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells
title_fullStr Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells
title_full_unstemmed Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells
title_short Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells
title_sort equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132922/
https://www.ncbi.nlm.nih.gov/pubmed/25088216
http://dx.doi.org/10.1186/1472-6882-14-283
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