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Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists

[Image: see text] The innate immune response is controlled, in part, by the synergistic interaction of multiple Toll-like receptors (TLRs). This multi-receptor cooperation is responsible for the potent activity of many vaccines, but few tools have been developed to understand the spatio-temporal ele...

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Autores principales: Ryu, Keun Ah, Stutts, Lalisa, Tom, Janine K., Mancini, Rock J., Esser-Kahn, Aaron P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132957/
https://www.ncbi.nlm.nih.gov/pubmed/25029205
http://dx.doi.org/10.1021/ja412314j
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author Ryu, Keun Ah
Stutts, Lalisa
Tom, Janine K.
Mancini, Rock J.
Esser-Kahn, Aaron P.
author_facet Ryu, Keun Ah
Stutts, Lalisa
Tom, Janine K.
Mancini, Rock J.
Esser-Kahn, Aaron P.
author_sort Ryu, Keun Ah
collection PubMed
description [Image: see text] The innate immune response is controlled, in part, by the synergistic interaction of multiple Toll-like receptors (TLRs). This multi-receptor cooperation is responsible for the potent activity of many vaccines, but few tools have been developed to understand the spatio-temporal elements of TLR synergies. In this Communication, we present photo-controlled agonists of TLR7/8. By strategically protecting the active agonist moiety based on an agonist-bound crystal structure, TLR activity is suppressed and then regained upon exposure to light. We confirmed NF-κB production upon light exposure in a model macrophage cell line. Primary cell activity was confirmed by examining cytokine and cell surface marker production in bone-marrow-derived dendritic cells. Finally, we used light to activate dendritic cell sub-populations within a larger population.
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spelling pubmed-41329572015-07-16 Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists Ryu, Keun Ah Stutts, Lalisa Tom, Janine K. Mancini, Rock J. Esser-Kahn, Aaron P. J Am Chem Soc [Image: see text] The innate immune response is controlled, in part, by the synergistic interaction of multiple Toll-like receptors (TLRs). This multi-receptor cooperation is responsible for the potent activity of many vaccines, but few tools have been developed to understand the spatio-temporal elements of TLR synergies. In this Communication, we present photo-controlled agonists of TLR7/8. By strategically protecting the active agonist moiety based on an agonist-bound crystal structure, TLR activity is suppressed and then regained upon exposure to light. We confirmed NF-κB production upon light exposure in a model macrophage cell line. Primary cell activity was confirmed by examining cytokine and cell surface marker production in bone-marrow-derived dendritic cells. Finally, we used light to activate dendritic cell sub-populations within a larger population. American Chemical Society 2014-07-16 2014-08-06 /pmc/articles/PMC4132957/ /pubmed/25029205 http://dx.doi.org/10.1021/ja412314j Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Ryu, Keun Ah
Stutts, Lalisa
Tom, Janine K.
Mancini, Rock J.
Esser-Kahn, Aaron P.
Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
title Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
title_full Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
title_fullStr Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
title_full_unstemmed Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
title_short Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
title_sort stimulation of innate immune cells by light-activated tlr7/8 agonists
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132957/
https://www.ncbi.nlm.nih.gov/pubmed/25029205
http://dx.doi.org/10.1021/ja412314j
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