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Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography

Speckle variance optical coherence angiography (OCA) was used to characterize the vascular tissue response from craniotomy, window implantation, and electrode insertion in mouse motor cortex. We observed initial vasodilation ~40% greater than original diameter 2-3 days post-surgery (dps). After 4 we...

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Autores principales: Hammer, Daniel X., Lozzi, Andrea, Abliz, Erkinay, Greenbaum, Noah, Agrawal, Anant, Krauthamer, Victor, Welle, Cristin G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133009/
https://www.ncbi.nlm.nih.gov/pubmed/25136505
http://dx.doi.org/10.1364/BOE.5.002823
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author Hammer, Daniel X.
Lozzi, Andrea
Abliz, Erkinay
Greenbaum, Noah
Agrawal, Anant
Krauthamer, Victor
Welle, Cristin G.
author_facet Hammer, Daniel X.
Lozzi, Andrea
Abliz, Erkinay
Greenbaum, Noah
Agrawal, Anant
Krauthamer, Victor
Welle, Cristin G.
author_sort Hammer, Daniel X.
collection PubMed
description Speckle variance optical coherence angiography (OCA) was used to characterize the vascular tissue response from craniotomy, window implantation, and electrode insertion in mouse motor cortex. We observed initial vasodilation ~40% greater than original diameter 2-3 days post-surgery (dps). After 4 weeks, dilation subsided in large vessels (>50 µm diameter) but persisted in smaller vessels (25-50 µm diameter). Neovascularization began 8-12 dps and vessel migration continued throughout the study. Vasodilation and neovascularization were primarily associated with craniotomy and window implantation rather than electrode insertion. Initial evidence of capillary re-mapping in the region surrounding the implanted electrode was manifest in OCA image dissimilarity. Further investigation, including higher resolution imaging, is required to validate the finding. Spontaneous lesions also occurred in many electrode animals, though the inception point appeared random and not directly associated with electrode insertion. OCA allows high resolution, label-free in vivo visualization of neurovascular tissue, which may help determine any biological contribution to chronic electrode signal degradation. Vascular and flow-based biomarkers can aid development of novel neural prostheses.
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spelling pubmed-41330092014-08-18 Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography Hammer, Daniel X. Lozzi, Andrea Abliz, Erkinay Greenbaum, Noah Agrawal, Anant Krauthamer, Victor Welle, Cristin G. Biomed Opt Express Article Speckle variance optical coherence angiography (OCA) was used to characterize the vascular tissue response from craniotomy, window implantation, and electrode insertion in mouse motor cortex. We observed initial vasodilation ~40% greater than original diameter 2-3 days post-surgery (dps). After 4 weeks, dilation subsided in large vessels (>50 µm diameter) but persisted in smaller vessels (25-50 µm diameter). Neovascularization began 8-12 dps and vessel migration continued throughout the study. Vasodilation and neovascularization were primarily associated with craniotomy and window implantation rather than electrode insertion. Initial evidence of capillary re-mapping in the region surrounding the implanted electrode was manifest in OCA image dissimilarity. Further investigation, including higher resolution imaging, is required to validate the finding. Spontaneous lesions also occurred in many electrode animals, though the inception point appeared random and not directly associated with electrode insertion. OCA allows high resolution, label-free in vivo visualization of neurovascular tissue, which may help determine any biological contribution to chronic electrode signal degradation. Vascular and flow-based biomarkers can aid development of novel neural prostheses. Optical Society of America 2014-07-28 /pmc/articles/PMC4133009/ /pubmed/25136505 http://dx.doi.org/10.1364/BOE.5.002823 Text en © 2014 Optical Society of America author-open
spellingShingle Article
Hammer, Daniel X.
Lozzi, Andrea
Abliz, Erkinay
Greenbaum, Noah
Agrawal, Anant
Krauthamer, Victor
Welle, Cristin G.
Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
title Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
title_full Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
title_fullStr Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
title_full_unstemmed Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
title_short Longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
title_sort longitudinal vascular dynamics following cranial window and electrode implantation measured with speckle variance optical coherence angiography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133009/
https://www.ncbi.nlm.nih.gov/pubmed/25136505
http://dx.doi.org/10.1364/BOE.5.002823
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