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Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells
KEY MESSAGE: PCC induction is regulated by several signaling pathways, and all observed effects associated with PCC induction are strongly dependent on the mechanism of action of each PCC inducer used. ABSTRACT: Electron microscopic observations of cells with symptoms of premature chromosome condens...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133037/ https://www.ncbi.nlm.nih.gov/pubmed/24898011 http://dx.doi.org/10.1007/s00299-014-1637-0 |
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author | Rybaczek, Dorota |
author_facet | Rybaczek, Dorota |
author_sort | Rybaczek, Dorota |
collection | PubMed |
description | KEY MESSAGE: PCC induction is regulated by several signaling pathways, and all observed effects associated with PCC induction are strongly dependent on the mechanism of action of each PCC inducer used. ABSTRACT: Electron microscopic observations of cells with symptoms of premature chromosome condensation (PCC) showed that the interphase chromatin and mitotic chromosomes differed with respect to a chemical compound inducing PCC. Induction of this process under the influence of hydroxyurea and caffeine as well as hydroxyurea and sodium metavanadate led to a slight decrease in interphase chromatin condensation and the formation of chromosomes with a considerably loosened structure in comparison with the control. Incubation in the mixture of hydroxyurea and 2-aminopurine brought about clear chromatin dispersion in interphase and very strong mitotic chromosome condensation. Electron microscopic examinations also revealed the characteristic features of the structural organization of cytoplasm of Vicia faba root meristems, which seemed to be dependent on the type of the PCC inducer used. The presence of the following was observed: (i) large plastids filled with starch grains (caffeine), (ii) mitochondria and plastids of electron dense matrix with dilated invaginations of their internal membranes (2-aminopurine), and (iii) large mitochondria of electron clear matrix and plastids containing protein crystals in their interior (sodium metavanadate). Moreover, since caffeine causes either the most effective loosening of chromatin fibrils (within the prematurely condensed chromosomes) or induction of starch formation (in the plastids surrounding the nuclei), this may be a proof that demonstrates the existence of a link between physical accessibility to chromatin and the effectiveness of cellular signaling (e.g., phosphothreonine-connected). |
format | Online Article Text |
id | pubmed-4133037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-41330372014-08-21 Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells Rybaczek, Dorota Plant Cell Rep Original Paper KEY MESSAGE: PCC induction is regulated by several signaling pathways, and all observed effects associated with PCC induction are strongly dependent on the mechanism of action of each PCC inducer used. ABSTRACT: Electron microscopic observations of cells with symptoms of premature chromosome condensation (PCC) showed that the interphase chromatin and mitotic chromosomes differed with respect to a chemical compound inducing PCC. Induction of this process under the influence of hydroxyurea and caffeine as well as hydroxyurea and sodium metavanadate led to a slight decrease in interphase chromatin condensation and the formation of chromosomes with a considerably loosened structure in comparison with the control. Incubation in the mixture of hydroxyurea and 2-aminopurine brought about clear chromatin dispersion in interphase and very strong mitotic chromosome condensation. Electron microscopic examinations also revealed the characteristic features of the structural organization of cytoplasm of Vicia faba root meristems, which seemed to be dependent on the type of the PCC inducer used. The presence of the following was observed: (i) large plastids filled with starch grains (caffeine), (ii) mitochondria and plastids of electron dense matrix with dilated invaginations of their internal membranes (2-aminopurine), and (iii) large mitochondria of electron clear matrix and plastids containing protein crystals in their interior (sodium metavanadate). Moreover, since caffeine causes either the most effective loosening of chromatin fibrils (within the prematurely condensed chromosomes) or induction of starch formation (in the plastids surrounding the nuclei), this may be a proof that demonstrates the existence of a link between physical accessibility to chromatin and the effectiveness of cellular signaling (e.g., phosphothreonine-connected). Springer Berlin Heidelberg 2014-06-05 2014 /pmc/articles/PMC4133037/ /pubmed/24898011 http://dx.doi.org/10.1007/s00299-014-1637-0 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Paper Rybaczek, Dorota Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells |
title | Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells |
title_full | Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells |
title_fullStr | Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells |
title_full_unstemmed | Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells |
title_short | Ultrastructural changes associated with the induction of premature chromosome condensation in Vicia faba root meristem cells |
title_sort | ultrastructural changes associated with the induction of premature chromosome condensation in vicia faba root meristem cells |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133037/ https://www.ncbi.nlm.nih.gov/pubmed/24898011 http://dx.doi.org/10.1007/s00299-014-1637-0 |
work_keys_str_mv | AT rybaczekdorota ultrastructuralchangesassociatedwiththeinductionofprematurechromosomecondensationinviciafabarootmeristemcells |