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Analysis of qPCR reference gene stability determination methods and a practical approach for efficiency calculation on a turbot (Scophthalmus maximus) gonad dataset

BACKGROUND: Gene expression analysis by reverse transcription quantitative PCR (qPCR) is the most widely used method for analyzing the expression of a moderate number of genes and also for the validation of microarray results. Several issues are crucial for a successful qPCR study, particularly the...

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Detalles Bibliográficos
Autores principales:	Robledo, Diego, Hernández-Urcera, Jorge, Cal, Rosa M, Pardo, Belén G, Sánchez, Laura, Martínez, Paulino, Viñas, Ana
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133071/ https://www.ncbi.nlm.nih.gov/pubmed/25091330 http://dx.doi.org/10.1186/1471-2164-15-648

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