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The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans

PGC-1α regulates critical processes in muscle physiology, including mitochondrial biogenesis, lipid metabolism and angiogenesis. Furthermore, PGC-1α was suggested as an important regulator of fiber type determination. However, whether a muscle fiber type-specific PGC-1α content exists, whether PGC-1...

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Autores principales: Gouspillou, Gilles, Sgarioto, Nicolas, Norris, Brandon, Barbat-Artigas, Sébastien, Aubertin-Leheudre, Mylène, Morais, Jose A., Burelle, Yan, Taivassalo, Tanja, Hepple, Russell T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133187/
https://www.ncbi.nlm.nih.gov/pubmed/25121500
http://dx.doi.org/10.1371/journal.pone.0103044
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author Gouspillou, Gilles
Sgarioto, Nicolas
Norris, Brandon
Barbat-Artigas, Sébastien
Aubertin-Leheudre, Mylène
Morais, Jose A.
Burelle, Yan
Taivassalo, Tanja
Hepple, Russell T.
author_facet Gouspillou, Gilles
Sgarioto, Nicolas
Norris, Brandon
Barbat-Artigas, Sébastien
Aubertin-Leheudre, Mylène
Morais, Jose A.
Burelle, Yan
Taivassalo, Tanja
Hepple, Russell T.
author_sort Gouspillou, Gilles
collection PubMed
description PGC-1α regulates critical processes in muscle physiology, including mitochondrial biogenesis, lipid metabolism and angiogenesis. Furthermore, PGC-1α was suggested as an important regulator of fiber type determination. However, whether a muscle fiber type-specific PGC-1α content exists, whether PGC-1α content relates to basal levels of mitochondrial content, and whether such relationships are preserved between humans and classically used rodent models are all questions that have been either poorly addressed or never investigated. To address these issues, we investigated the fiber type-specific content of PGC-1α and its relationship to basal mitochondrial content in mouse, rat and human muscles using in situ immunolabeling and histochemical methods on muscle serial cross-sections. Whereas type IIa fibers exhibited the highest PGC-1α in all three species, other fiber types displayed a hierarchy of type IIx>I>IIb in mouse, type I = IIx> IIb in rat, and type IIx>I in human. In terms of mitochondrial content, we observed a hierarchy of IIa>IIx>I>IIb in mouse, IIa >I>IIx> IIb in rat, and I>IIa> IIx in human skeletal muscle. We also found in rat skeletal muscle that type I fibers displayed the highest capillarization followed by type IIa >IIx>IIb. Finally, we found in human skeletal muscle that type I fibers display the highest lipid content, followed by type IIa>IIx. Altogether, our results reveal that (i) the fiber type-specific PGC-1α and mitochondrial contents were only matched in mouse, (ii) the patterns of PGC-1α and mitochondrial contents observed in mice and rats do not correspond to that seen in humans in several respects, and (iii) the classical phenotypes thought to be regulated by PGC-1α do not vary exclusively as a function of PGC-1α content in rat and human muscles.
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spelling pubmed-41331872014-08-19 The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans Gouspillou, Gilles Sgarioto, Nicolas Norris, Brandon Barbat-Artigas, Sébastien Aubertin-Leheudre, Mylène Morais, Jose A. Burelle, Yan Taivassalo, Tanja Hepple, Russell T. PLoS One Research Article PGC-1α regulates critical processes in muscle physiology, including mitochondrial biogenesis, lipid metabolism and angiogenesis. Furthermore, PGC-1α was suggested as an important regulator of fiber type determination. However, whether a muscle fiber type-specific PGC-1α content exists, whether PGC-1α content relates to basal levels of mitochondrial content, and whether such relationships are preserved between humans and classically used rodent models are all questions that have been either poorly addressed or never investigated. To address these issues, we investigated the fiber type-specific content of PGC-1α and its relationship to basal mitochondrial content in mouse, rat and human muscles using in situ immunolabeling and histochemical methods on muscle serial cross-sections. Whereas type IIa fibers exhibited the highest PGC-1α in all three species, other fiber types displayed a hierarchy of type IIx>I>IIb in mouse, type I = IIx> IIb in rat, and type IIx>I in human. In terms of mitochondrial content, we observed a hierarchy of IIa>IIx>I>IIb in mouse, IIa >I>IIx> IIb in rat, and I>IIa> IIx in human skeletal muscle. We also found in rat skeletal muscle that type I fibers displayed the highest capillarization followed by type IIa >IIx>IIb. Finally, we found in human skeletal muscle that type I fibers display the highest lipid content, followed by type IIa>IIx. Altogether, our results reveal that (i) the fiber type-specific PGC-1α and mitochondrial contents were only matched in mouse, (ii) the patterns of PGC-1α and mitochondrial contents observed in mice and rats do not correspond to that seen in humans in several respects, and (iii) the classical phenotypes thought to be regulated by PGC-1α do not vary exclusively as a function of PGC-1α content in rat and human muscles. Public Library of Science 2014-08-14 /pmc/articles/PMC4133187/ /pubmed/25121500 http://dx.doi.org/10.1371/journal.pone.0103044 Text en © 2014 Gouspillou et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gouspillou, Gilles
Sgarioto, Nicolas
Norris, Brandon
Barbat-Artigas, Sébastien
Aubertin-Leheudre, Mylène
Morais, Jose A.
Burelle, Yan
Taivassalo, Tanja
Hepple, Russell T.
The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans
title The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans
title_full The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans
title_fullStr The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans
title_full_unstemmed The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans
title_short The Relationship between Muscle Fiber Type-Specific PGC-1α Content and Mitochondrial Content Varies between Rodent Models and Humans
title_sort relationship between muscle fiber type-specific pgc-1α content and mitochondrial content varies between rodent models and humans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133187/
https://www.ncbi.nlm.nih.gov/pubmed/25121500
http://dx.doi.org/10.1371/journal.pone.0103044
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