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White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays

Prion transmission can occur by blood transfusion in human variant Creutzfeldt-Jakob disease and in experimental animal models, including sheep. Screening of blood and its derivatives for the presence of prions became therefore a major public health issue. As infectious titer in blood is reportedly...

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Autores principales: Halliez, Sophie, Jaumain, Emilie, Huor, Alvina, Douet, Jean-Yves, Lugan, Séverine, Cassard, Hervé, Lacroux, Caroline, Béringue, Vincent, Andréoletti, Olivier, Vilette, Didier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133197/
https://www.ncbi.nlm.nih.gov/pubmed/25122456
http://dx.doi.org/10.1371/journal.pone.0104287
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author Halliez, Sophie
Jaumain, Emilie
Huor, Alvina
Douet, Jean-Yves
Lugan, Séverine
Cassard, Hervé
Lacroux, Caroline
Béringue, Vincent
Andréoletti, Olivier
Vilette, Didier
author_facet Halliez, Sophie
Jaumain, Emilie
Huor, Alvina
Douet, Jean-Yves
Lugan, Séverine
Cassard, Hervé
Lacroux, Caroline
Béringue, Vincent
Andréoletti, Olivier
Vilette, Didier
author_sort Halliez, Sophie
collection PubMed
description Prion transmission can occur by blood transfusion in human variant Creutzfeldt-Jakob disease and in experimental animal models, including sheep. Screening of blood and its derivatives for the presence of prions became therefore a major public health issue. As infectious titer in blood is reportedly low, highly sensitive and robust methods are required to detect prions in blood and blood derived products. The objectives of this study were to compare different methods - in vitro, ex vivo and in vivo assays - to detect prion infectivity in cells prepared from blood samples obtained from scrapie infected sheep at different time points of the disease. Protein misfolding cyclic amplification (PMCA) and bioassays in transgenic mice expressing the ovine prion protein were the most efficient methods to identify infected animals at any time of the disease (asymptomatic to terminally-ill stages). However scrapie cell and cerebellar organotypic slice culture assays designed to replicate ovine prions in culture also allowed detection of prion infectivity in blood cells from asymptomatic sheep. These findings confirm that white blood cells are appropriate targets for preclinical detection and introduce ex vivo tools to detect blood infectivity during the asymptomatic stage of the disease.
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spelling pubmed-41331972014-08-19 White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays Halliez, Sophie Jaumain, Emilie Huor, Alvina Douet, Jean-Yves Lugan, Séverine Cassard, Hervé Lacroux, Caroline Béringue, Vincent Andréoletti, Olivier Vilette, Didier PLoS One Research Article Prion transmission can occur by blood transfusion in human variant Creutzfeldt-Jakob disease and in experimental animal models, including sheep. Screening of blood and its derivatives for the presence of prions became therefore a major public health issue. As infectious titer in blood is reportedly low, highly sensitive and robust methods are required to detect prions in blood and blood derived products. The objectives of this study were to compare different methods - in vitro, ex vivo and in vivo assays - to detect prion infectivity in cells prepared from blood samples obtained from scrapie infected sheep at different time points of the disease. Protein misfolding cyclic amplification (PMCA) and bioassays in transgenic mice expressing the ovine prion protein were the most efficient methods to identify infected animals at any time of the disease (asymptomatic to terminally-ill stages). However scrapie cell and cerebellar organotypic slice culture assays designed to replicate ovine prions in culture also allowed detection of prion infectivity in blood cells from asymptomatic sheep. These findings confirm that white blood cells are appropriate targets for preclinical detection and introduce ex vivo tools to detect blood infectivity during the asymptomatic stage of the disease. Public Library of Science 2014-08-14 /pmc/articles/PMC4133197/ /pubmed/25122456 http://dx.doi.org/10.1371/journal.pone.0104287 Text en © 2014 Halliez et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Halliez, Sophie
Jaumain, Emilie
Huor, Alvina
Douet, Jean-Yves
Lugan, Séverine
Cassard, Hervé
Lacroux, Caroline
Béringue, Vincent
Andréoletti, Olivier
Vilette, Didier
White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays
title White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays
title_full White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays
title_fullStr White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays
title_full_unstemmed White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays
title_short White Blood Cell-Based Detection of Asymptomatic Scrapie Infection by Ex Vivo Assays
title_sort white blood cell-based detection of asymptomatic scrapie infection by ex vivo assays
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133197/
https://www.ncbi.nlm.nih.gov/pubmed/25122456
http://dx.doi.org/10.1371/journal.pone.0104287
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