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Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen
Pseudomonas aeruginosa is one of the pathogenic bacteria which utilize binding of the host plasminogen (Plg) to promote their invasion throughout the host tissues. In the present study, we confirmed that P. aeruginosa exhibits binding affinity for human plasminogen. Furthermore, we showed that the p...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133640/ https://www.ncbi.nlm.nih.gov/pubmed/24671511 http://dx.doi.org/10.1007/s12223-014-0311-9 |
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author | Ceremuga, Ireneusz Seweryn, Ewa Bednarz-Misa, Iwona Pietkiewicz, Jadwiga Jermakow, Katarzyna Banaś, Teresa Gamian, Andrzej |
author_facet | Ceremuga, Ireneusz Seweryn, Ewa Bednarz-Misa, Iwona Pietkiewicz, Jadwiga Jermakow, Katarzyna Banaś, Teresa Gamian, Andrzej |
author_sort | Ceremuga, Ireneusz |
collection | PubMed |
description | Pseudomonas aeruginosa is one of the pathogenic bacteria which utilize binding of the host plasminogen (Plg) to promote their invasion throughout the host tissues. In the present study, we confirmed that P. aeruginosa exhibits binding affinity for human plasminogen. Furthermore, we showed that the protein detected on the cell wall of P. aeruginosa and binding human plasminogen is an enolase-like protein. The hypothesis that alpha-enolase, a cytoplasmatic glycolytic enzyme, resides also on the cell surface of the bacterium was supported by electron microscopy analysis. The plasminogen-binding activity of bacterial cell wall outer membrane enolase-like protein was examined by immunoblotting assay. |
format | Online Article Text |
id | pubmed-4133640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-41336402014-08-21 Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen Ceremuga, Ireneusz Seweryn, Ewa Bednarz-Misa, Iwona Pietkiewicz, Jadwiga Jermakow, Katarzyna Banaś, Teresa Gamian, Andrzej Folia Microbiol (Praha) Article Pseudomonas aeruginosa is one of the pathogenic bacteria which utilize binding of the host plasminogen (Plg) to promote their invasion throughout the host tissues. In the present study, we confirmed that P. aeruginosa exhibits binding affinity for human plasminogen. Furthermore, we showed that the protein detected on the cell wall of P. aeruginosa and binding human plasminogen is an enolase-like protein. The hypothesis that alpha-enolase, a cytoplasmatic glycolytic enzyme, resides also on the cell surface of the bacterium was supported by electron microscopy analysis. The plasminogen-binding activity of bacterial cell wall outer membrane enolase-like protein was examined by immunoblotting assay. Springer Netherlands 2014-03-27 2014 /pmc/articles/PMC4133640/ /pubmed/24671511 http://dx.doi.org/10.1007/s12223-014-0311-9 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Article Ceremuga, Ireneusz Seweryn, Ewa Bednarz-Misa, Iwona Pietkiewicz, Jadwiga Jermakow, Katarzyna Banaś, Teresa Gamian, Andrzej Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen |
title | Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen |
title_full | Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen |
title_fullStr | Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen |
title_full_unstemmed | Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen |
title_short | Enolase-like protein present on the outer membrane of Pseudomonas aeruginosa binds plasminogen |
title_sort | enolase-like protein present on the outer membrane of pseudomonas aeruginosa binds plasminogen |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133640/ https://www.ncbi.nlm.nih.gov/pubmed/24671511 http://dx.doi.org/10.1007/s12223-014-0311-9 |
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