In vivo Magnetic Resonance Imaging of Tumor Protease Activity

Increased expression of cathepsins has diagnostic as well as prognostic value in several types of cancer. Here, we demonstrate a novel magnetic resonance imaging (MRI) method, which uses poly-L-glutamate (PLG) as an MRI probe to map cathepsin expression in vivo, in a rat brain tumor model. This noni...

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Autores principales: Haris, Mohammad, Singh, Anup, Mohammed, Imran, Ittyerah, Ranjit, Nath, Kavindra, Nanga, Ravi Prakash Reddy, Debrosse, Catherine, Kogan, Feliks, Cai, Kejia, Poptani, Harish, Reddy, Damodar, Hariharan, Hari, Reddy, Ravinder
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133714/
https://www.ncbi.nlm.nih.gov/pubmed/25124082
http://dx.doi.org/10.1038/srep06081
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author Haris, Mohammad
Singh, Anup
Mohammed, Imran
Ittyerah, Ranjit
Nath, Kavindra
Nanga, Ravi Prakash Reddy
Debrosse, Catherine
Kogan, Feliks
Cai, Kejia
Poptani, Harish
Reddy, Damodar
Hariharan, Hari
Reddy, Ravinder
author_facet Haris, Mohammad
Singh, Anup
Mohammed, Imran
Ittyerah, Ranjit
Nath, Kavindra
Nanga, Ravi Prakash Reddy
Debrosse, Catherine
Kogan, Feliks
Cai, Kejia
Poptani, Harish
Reddy, Damodar
Hariharan, Hari
Reddy, Ravinder
author_sort Haris, Mohammad
collection PubMed
description Increased expression of cathepsins has diagnostic as well as prognostic value in several types of cancer. Here, we demonstrate a novel magnetic resonance imaging (MRI) method, which uses poly-L-glutamate (PLG) as an MRI probe to map cathepsin expression in vivo, in a rat brain tumor model. This noninvasive, high-resolution and non-radioactive method exploits the differences in the CEST signals of PLG in the native form and cathepsin mediated cleaved form. The method was validated in phantoms with known physiological concentrations, in tumor cells and in an animal model of brain tumor along with immunohistochemical analysis. Potential applications in tumor diagnosis and evaluation of therapeutic response are outlined.
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spelling pubmed-41337142014-08-18 In vivo Magnetic Resonance Imaging of Tumor Protease Activity Haris, Mohammad Singh, Anup Mohammed, Imran Ittyerah, Ranjit Nath, Kavindra Nanga, Ravi Prakash Reddy Debrosse, Catherine Kogan, Feliks Cai, Kejia Poptani, Harish Reddy, Damodar Hariharan, Hari Reddy, Ravinder Sci Rep Article Increased expression of cathepsins has diagnostic as well as prognostic value in several types of cancer. Here, we demonstrate a novel magnetic resonance imaging (MRI) method, which uses poly-L-glutamate (PLG) as an MRI probe to map cathepsin expression in vivo, in a rat brain tumor model. This noninvasive, high-resolution and non-radioactive method exploits the differences in the CEST signals of PLG in the native form and cathepsin mediated cleaved form. The method was validated in phantoms with known physiological concentrations, in tumor cells and in an animal model of brain tumor along with immunohistochemical analysis. Potential applications in tumor diagnosis and evaluation of therapeutic response are outlined. Nature Publishing Group 2014-08-15 /pmc/articles/PMC4133714/ /pubmed/25124082 http://dx.doi.org/10.1038/srep06081 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Haris, Mohammad
Singh, Anup
Mohammed, Imran
Ittyerah, Ranjit
Nath, Kavindra
Nanga, Ravi Prakash Reddy
Debrosse, Catherine
Kogan, Feliks
Cai, Kejia
Poptani, Harish
Reddy, Damodar
Hariharan, Hari
Reddy, Ravinder
In vivo Magnetic Resonance Imaging of Tumor Protease Activity
title In vivo Magnetic Resonance Imaging of Tumor Protease Activity
title_full In vivo Magnetic Resonance Imaging of Tumor Protease Activity
title_fullStr In vivo Magnetic Resonance Imaging of Tumor Protease Activity
title_full_unstemmed In vivo Magnetic Resonance Imaging of Tumor Protease Activity
title_short In vivo Magnetic Resonance Imaging of Tumor Protease Activity
title_sort in vivo magnetic resonance imaging of tumor protease activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133714/
https://www.ncbi.nlm.nih.gov/pubmed/25124082
http://dx.doi.org/10.1038/srep06081
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