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Extraction of human genomic DNA from whole blood using a magnetic microsphere method
With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134021/ https://www.ncbi.nlm.nih.gov/pubmed/25143727 http://dx.doi.org/10.2147/IJN.S59545 |
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author | Gong, Rui Li, Shengying |
author_facet | Gong, Rui Li, Shengying |
author_sort | Gong, Rui |
collection | PubMed |
description | With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time‐consuming, and phenol and chloroform are toxic reagents that endanger health. Therefore, it is necessary to find a more convenient and efficient method for obtaining human genomic DNA. In this study, we developed urea–formaldehyde resin magnetic microspheres and magnetic silica microspheres for extraction of human genomic DNA. First, a magnetic microsphere suspension was prepared and used to extract genomic DNA from fresh whole blood, frozen blood, dried blood, and trace blood. Second, DNA content and purity were measured by agarose electrophoresis and ultraviolet spectrophotometry. The human genomic DNA extracted from whole blood was then subjected to polymerase chain reaction analysis to further confirm its quality. The results of this study lay a good foundation for future research and development of a high‐throughput and rapid extraction method for extracting genomic DNA from various types of blood samples. |
format | Online Article Text |
id | pubmed-4134021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41340212014-08-20 Extraction of human genomic DNA from whole blood using a magnetic microsphere method Gong, Rui Li, Shengying Int J Nanomedicine Original Research With the rapid development of molecular biology and the life sciences, magnetic extraction is a simple, automatic, and highly efficient method for separating biological molecules, performing immunoassays, and other applications. Human blood is an ideal source of human genomic DNA. Extracting genomic DNA by traditional methods is time‐consuming, and phenol and chloroform are toxic reagents that endanger health. Therefore, it is necessary to find a more convenient and efficient method for obtaining human genomic DNA. In this study, we developed urea–formaldehyde resin magnetic microspheres and magnetic silica microspheres for extraction of human genomic DNA. First, a magnetic microsphere suspension was prepared and used to extract genomic DNA from fresh whole blood, frozen blood, dried blood, and trace blood. Second, DNA content and purity were measured by agarose electrophoresis and ultraviolet spectrophotometry. The human genomic DNA extracted from whole blood was then subjected to polymerase chain reaction analysis to further confirm its quality. The results of this study lay a good foundation for future research and development of a high‐throughput and rapid extraction method for extracting genomic DNA from various types of blood samples. Dove Medical Press 2014-08-08 /pmc/articles/PMC4134021/ /pubmed/25143727 http://dx.doi.org/10.2147/IJN.S59545 Text en © 2014 Gong and Li. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Gong, Rui Li, Shengying Extraction of human genomic DNA from whole blood using a magnetic microsphere method |
title | Extraction of human genomic DNA from whole blood using a magnetic microsphere method |
title_full | Extraction of human genomic DNA from whole blood using a magnetic microsphere method |
title_fullStr | Extraction of human genomic DNA from whole blood using a magnetic microsphere method |
title_full_unstemmed | Extraction of human genomic DNA from whole blood using a magnetic microsphere method |
title_short | Extraction of human genomic DNA from whole blood using a magnetic microsphere method |
title_sort | extraction of human genomic dna from whole blood using a magnetic microsphere method |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134021/ https://www.ncbi.nlm.nih.gov/pubmed/25143727 http://dx.doi.org/10.2147/IJN.S59545 |
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