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Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method

To establish a satisfactory delivery system for the delivery of salinomycin (Sal), a novel, selective cancer stem cell inhibitor with prominent toxicity, gelatinase-responsive core-shell nanoparticles (NPs), were prepared by nanoprecipitation method (NR-NPs) and single emulsion method (SE-NPs). The...

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Autores principales: Wang, Qin, Wu, Puyuan, Ren, Wei, Xin, Kai, Yang, Yang, Xie, Chen, Yang, Chenchen, Liu, Qin, Yu, Lixia, Jiang, Xiqun, Liu, Baorui, Li, Rutain, Wang, Lifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134115/
https://www.ncbi.nlm.nih.gov/pubmed/25147486
http://dx.doi.org/10.1186/1556-276X-9-351
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author Wang, Qin
Wu, Puyuan
Ren, Wei
Xin, Kai
Yang, Yang
Xie, Chen
Yang, Chenchen
Liu, Qin
Yu, Lixia
Jiang, Xiqun
Liu, Baorui
Li, Rutain
Wang, Lifeng
author_facet Wang, Qin
Wu, Puyuan
Ren, Wei
Xin, Kai
Yang, Yang
Xie, Chen
Yang, Chenchen
Liu, Qin
Yu, Lixia
Jiang, Xiqun
Liu, Baorui
Li, Rutain
Wang, Lifeng
author_sort Wang, Qin
collection PubMed
description To establish a satisfactory delivery system for the delivery of salinomycin (Sal), a novel, selective cancer stem cell inhibitor with prominent toxicity, gelatinase-responsive core-shell nanoparticles (NPs), were prepared by nanoprecipitation method (NR-NPs) and single emulsion method (SE-NPs). The gelatinase-responsive copolymer was prepared by carboxylation and double amination method. We studied the stability of NPs prepared by nanoprecipitation method with different proportions of F68 in aqueous phase to determine the best proportion used in our study. Then, the NPs were prepared by nanoprecipitation method with the best proportion of F68 and single emulsion method, and their physiochemical traits including morphology, particle size, zeta potential, drug loading content, stability, and in vitro release profiles were studied. The SE-NPs showed significant differences in particle size, drug loading content, stability, and in vitro release profiles compared to NR-NPs. The SE-NPs presented higher drug entrapment efficiency and superior stability than the NR-NPs. The drug release rate of SE-NPs was more sustainable than that of the NR-NPs, and in vivo experiment indicated that NPs could prominently reduce the toxicity of Sal. Our study demonstrates that the SE-NPs could be a satisfactory method for the preparation of gelatinase-responsive NPs for intelligent delivery of Sal.
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spelling pubmed-41341152014-08-21 Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method Wang, Qin Wu, Puyuan Ren, Wei Xin, Kai Yang, Yang Xie, Chen Yang, Chenchen Liu, Qin Yu, Lixia Jiang, Xiqun Liu, Baorui Li, Rutain Wang, Lifeng Nanoscale Res Lett Nano Express To establish a satisfactory delivery system for the delivery of salinomycin (Sal), a novel, selective cancer stem cell inhibitor with prominent toxicity, gelatinase-responsive core-shell nanoparticles (NPs), were prepared by nanoprecipitation method (NR-NPs) and single emulsion method (SE-NPs). The gelatinase-responsive copolymer was prepared by carboxylation and double amination method. We studied the stability of NPs prepared by nanoprecipitation method with different proportions of F68 in aqueous phase to determine the best proportion used in our study. Then, the NPs were prepared by nanoprecipitation method with the best proportion of F68 and single emulsion method, and their physiochemical traits including morphology, particle size, zeta potential, drug loading content, stability, and in vitro release profiles were studied. The SE-NPs showed significant differences in particle size, drug loading content, stability, and in vitro release profiles compared to NR-NPs. The SE-NPs presented higher drug entrapment efficiency and superior stability than the NR-NPs. The drug release rate of SE-NPs was more sustainable than that of the NR-NPs, and in vivo experiment indicated that NPs could prominently reduce the toxicity of Sal. Our study demonstrates that the SE-NPs could be a satisfactory method for the preparation of gelatinase-responsive NPs for intelligent delivery of Sal. Springer 2014-07-15 /pmc/articles/PMC4134115/ /pubmed/25147486 http://dx.doi.org/10.1186/1556-276X-9-351 Text en Copyright © 2014 Wang et al.; licensee Springer. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.
spellingShingle Nano Express
Wang, Qin
Wu, Puyuan
Ren, Wei
Xin, Kai
Yang, Yang
Xie, Chen
Yang, Chenchen
Liu, Qin
Yu, Lixia
Jiang, Xiqun
Liu, Baorui
Li, Rutain
Wang, Lifeng
Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method
title Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method
title_full Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method
title_fullStr Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method
title_full_unstemmed Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method
title_short Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method
title_sort comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134115/
https://www.ncbi.nlm.nih.gov/pubmed/25147486
http://dx.doi.org/10.1186/1556-276X-9-351
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