Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors

[Image: see text] Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiologic...

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Autores principales: Ulissi, Zachary W., Sen, Fatih, Gong, Xun, Sen, Selda, Iverson, Nicole, Boghossian, Ardemis A., Godoy, Luiz C., Wogan, Gerald N., Mukhopadhyay, Debabrata, Strano, Michael S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134139/
https://www.ncbi.nlm.nih.gov/pubmed/25029087
http://dx.doi.org/10.1021/nl502338y
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author Ulissi, Zachary W.
Sen, Fatih
Gong, Xun
Sen, Selda
Iverson, Nicole
Boghossian, Ardemis A.
Godoy, Luiz C.
Wogan, Gerald N.
Mukhopadhyay, Debabrata
Strano, Michael S.
author_facet Ulissi, Zachary W.
Sen, Fatih
Gong, Xun
Sen, Selda
Iverson, Nicole
Boghossian, Ardemis A.
Godoy, Luiz C.
Wogan, Gerald N.
Mukhopadhyay, Debabrata
Strano, Michael S.
author_sort Ulissi, Zachary W.
collection PubMed
description [Image: see text] Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics.
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spelling pubmed-41341392015-07-16 Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors Ulissi, Zachary W. Sen, Fatih Gong, Xun Sen, Selda Iverson, Nicole Boghossian, Ardemis A. Godoy, Luiz C. Wogan, Gerald N. Mukhopadhyay, Debabrata Strano, Michael S. Nano Lett [Image: see text] Fluorescent nanosensor probes have suffered from limited molecular recognition and a dearth of strategies for spatial-temporal operation in cell culture. In this work, we spatially imaged the dynamics of nitric oxide (NO) signaling, important in numerous pathologies and physiological functions, using intracellular near-infrared fluorescent single-walled carbon nanotubes. The observed spatial-temporal NO signaling gradients clarify and refine the existing paradigm of NO signaling based on averaged local concentrations. This work enables the study of transient intracellular phenomena associated with signaling and therapeutics. American Chemical Society 2014-07-16 2014-08-13 /pmc/articles/PMC4134139/ /pubmed/25029087 http://dx.doi.org/10.1021/nl502338y Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Ulissi, Zachary W.
Sen, Fatih
Gong, Xun
Sen, Selda
Iverson, Nicole
Boghossian, Ardemis A.
Godoy, Luiz C.
Wogan, Gerald N.
Mukhopadhyay, Debabrata
Strano, Michael S.
Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors
title Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors
title_full Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors
title_fullStr Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors
title_full_unstemmed Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors
title_short Spatiotemporal Intracellular Nitric Oxide Signaling Captured Using Internalized, Near-Infrared Fluorescent Carbon Nanotube Nanosensors
title_sort spatiotemporal intracellular nitric oxide signaling captured using internalized, near-infrared fluorescent carbon nanotube nanosensors
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134139/
https://www.ncbi.nlm.nih.gov/pubmed/25029087
http://dx.doi.org/10.1021/nl502338y
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