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Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads

In this work, lipase from Candida rugosa was immobilized onto chitosan/graphene oxide beads. This was to provide an enzyme-immobilizing carrier with excellent enzyme immobilization activity for an enzyme group requiring hydrophilicity on the immobilizing carrier. In addition, this work involved a pr...

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Autores principales: Lau, Siaw Cheng, Lim, Hong Ngee, Basri, Mahiran, Fard Masoumi, Hamid Reza, Ahmad Tajudin, Asilah, Huang, Nay Ming, Pandikumar, Alagarsamy, Chia, Chi Hua, Andou, Yoshito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134220/
https://www.ncbi.nlm.nih.gov/pubmed/25127038
http://dx.doi.org/10.1371/journal.pone.0104695
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author Lau, Siaw Cheng
Lim, Hong Ngee
Basri, Mahiran
Fard Masoumi, Hamid Reza
Ahmad Tajudin, Asilah
Huang, Nay Ming
Pandikumar, Alagarsamy
Chia, Chi Hua
Andou, Yoshito
author_facet Lau, Siaw Cheng
Lim, Hong Ngee
Basri, Mahiran
Fard Masoumi, Hamid Reza
Ahmad Tajudin, Asilah
Huang, Nay Ming
Pandikumar, Alagarsamy
Chia, Chi Hua
Andou, Yoshito
author_sort Lau, Siaw Cheng
collection PubMed
description In this work, lipase from Candida rugosa was immobilized onto chitosan/graphene oxide beads. This was to provide an enzyme-immobilizing carrier with excellent enzyme immobilization activity for an enzyme group requiring hydrophilicity on the immobilizing carrier. In addition, this work involved a process for the preparation of an enzymatically active product insoluble in a reaction medium consisting of lauric acid and oleyl alcohol as reactants and hexane as a solvent. This product enabled the stability of the enzyme under the working conditions and allowed the enzyme to be readily isolated from the support. In particular, this meant that an enzymatic reaction could be stopped by the simple mechanical separation of the “insoluble” enzyme from the reaction medium. Chitosan was incorporated with graphene oxide because the latter was able to enhance the physical strength of the chitosan beads by its superior mechanical integrity and low thermal conductivity. The X-ray diffraction pattern showed that the graphene oxide was successfully embedded within the structure of the chitosan. Further, the lipase incorporation on the beads was confirmed by a thermo-gravimetric analysis. The lipase immobilization on the beads involved the functionalization with coupling agents, N-hydroxysulfosuccinimide sodium (NHS) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC), and it possessed a high enzyme activity of 64 U. The overall esterification conversion of the prepared product was 78% at 60°C, and it attained conversions of 98% and 88% with commercially available lipozyme and novozyme, respectively, under similar experimental conditions.
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spelling pubmed-41342202014-08-19 Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads Lau, Siaw Cheng Lim, Hong Ngee Basri, Mahiran Fard Masoumi, Hamid Reza Ahmad Tajudin, Asilah Huang, Nay Ming Pandikumar, Alagarsamy Chia, Chi Hua Andou, Yoshito PLoS One Research Article In this work, lipase from Candida rugosa was immobilized onto chitosan/graphene oxide beads. This was to provide an enzyme-immobilizing carrier with excellent enzyme immobilization activity for an enzyme group requiring hydrophilicity on the immobilizing carrier. In addition, this work involved a process for the preparation of an enzymatically active product insoluble in a reaction medium consisting of lauric acid and oleyl alcohol as reactants and hexane as a solvent. This product enabled the stability of the enzyme under the working conditions and allowed the enzyme to be readily isolated from the support. In particular, this meant that an enzymatic reaction could be stopped by the simple mechanical separation of the “insoluble” enzyme from the reaction medium. Chitosan was incorporated with graphene oxide because the latter was able to enhance the physical strength of the chitosan beads by its superior mechanical integrity and low thermal conductivity. The X-ray diffraction pattern showed that the graphene oxide was successfully embedded within the structure of the chitosan. Further, the lipase incorporation on the beads was confirmed by a thermo-gravimetric analysis. The lipase immobilization on the beads involved the functionalization with coupling agents, N-hydroxysulfosuccinimide sodium (NHS) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC), and it possessed a high enzyme activity of 64 U. The overall esterification conversion of the prepared product was 78% at 60°C, and it attained conversions of 98% and 88% with commercially available lipozyme and novozyme, respectively, under similar experimental conditions. Public Library of Science 2014-08-15 /pmc/articles/PMC4134220/ /pubmed/25127038 http://dx.doi.org/10.1371/journal.pone.0104695 Text en © 2014 Lau et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lau, Siaw Cheng
Lim, Hong Ngee
Basri, Mahiran
Fard Masoumi, Hamid Reza
Ahmad Tajudin, Asilah
Huang, Nay Ming
Pandikumar, Alagarsamy
Chia, Chi Hua
Andou, Yoshito
Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads
title Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads
title_full Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads
title_fullStr Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads
title_full_unstemmed Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads
title_short Enhanced Biocatalytic Esterification with Lipase-Immobilized Chitosan/Graphene Oxide Beads
title_sort enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134220/
https://www.ncbi.nlm.nih.gov/pubmed/25127038
http://dx.doi.org/10.1371/journal.pone.0104695
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