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Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation. This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134791/ https://www.ncbi.nlm.nih.gov/pubmed/25147833 http://dx.doi.org/10.1155/2014/125353 |
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author | Majid, Mohd Zabidi Mohamad Zaini, Zuraiza Abdul Razak, Fathilah |
author_facet | Majid, Mohd Zabidi Mohamad Zaini, Zuraiza Abdul Razak, Fathilah |
author_sort | Majid, Mohd Zabidi |
collection | PubMed |
description | Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation. This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their mode of cell death. The concentration producing 50% cell inhibition (IC(50)) was determined and the activity was examined under an inverted microscope. Immunohistochemistry fluorescent staining method (TUNEL) was performed to indicate the mechanism of cell death and the fragmented DNA band pattern produced was obtained for verification. Compared to Azadirachta sp. and Typhonium sp., the antiproliferative activity of Brucea sp. extract was the most potent on both KB and ORL-48 cells with IC(50) of 24.37 ± 1.75 and 6.67 ± 1.15 µg/mL, respectively. Signs of cell attrition were observed 24 hr after treatment. Green fluorescent spots indicating cell death by apoptosis were observed in images of both cells following treatment with all the three extracts. DNA fragments harvested from Brucea-treated cells produced bands in a ladder pattern suggesting the apoptotic effect of the extract. It is thus concluded that Brucea sp. extract exhibited cytotoxic activity on ORL-48 cells and their action mechanism is via apoptosis. |
format | Online Article Text |
id | pubmed-4134791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41347912014-08-21 Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 Majid, Mohd Zabidi Mohamad Zaini, Zuraiza Abdul Razak, Fathilah ScientificWorldJournal Research Article Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation. This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their mode of cell death. The concentration producing 50% cell inhibition (IC(50)) was determined and the activity was examined under an inverted microscope. Immunohistochemistry fluorescent staining method (TUNEL) was performed to indicate the mechanism of cell death and the fragmented DNA band pattern produced was obtained for verification. Compared to Azadirachta sp. and Typhonium sp., the antiproliferative activity of Brucea sp. extract was the most potent on both KB and ORL-48 cells with IC(50) of 24.37 ± 1.75 and 6.67 ± 1.15 µg/mL, respectively. Signs of cell attrition were observed 24 hr after treatment. Green fluorescent spots indicating cell death by apoptosis were observed in images of both cells following treatment with all the three extracts. DNA fragments harvested from Brucea-treated cells produced bands in a ladder pattern suggesting the apoptotic effect of the extract. It is thus concluded that Brucea sp. extract exhibited cytotoxic activity on ORL-48 cells and their action mechanism is via apoptosis. Hindawi Publishing Corporation 2014 2014-07-24 /pmc/articles/PMC4134791/ /pubmed/25147833 http://dx.doi.org/10.1155/2014/125353 Text en Copyright © 2014 Mohd Zabidi Majid et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Majid, Mohd Zabidi Mohamad Zaini, Zuraiza Abdul Razak, Fathilah Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 |
title | Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 |
title_full | Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 |
title_fullStr | Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 |
title_full_unstemmed | Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 |
title_short | Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 |
title_sort | apoptosis-inducing effect of three medicinal plants on oral cancer cells kb and orl-48 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134791/ https://www.ncbi.nlm.nih.gov/pubmed/25147833 http://dx.doi.org/10.1155/2014/125353 |
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