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Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48

Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation. This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their...

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Autores principales: Majid, Mohd Zabidi, Mohamad Zaini, Zuraiza, Abdul Razak, Fathilah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134791/
https://www.ncbi.nlm.nih.gov/pubmed/25147833
http://dx.doi.org/10.1155/2014/125353
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author Majid, Mohd Zabidi
Mohamad Zaini, Zuraiza
Abdul Razak, Fathilah
author_facet Majid, Mohd Zabidi
Mohamad Zaini, Zuraiza
Abdul Razak, Fathilah
author_sort Majid, Mohd Zabidi
collection PubMed
description Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation. This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their mode of cell death. The concentration producing 50% cell inhibition (IC(50)) was determined and the activity was examined under an inverted microscope. Immunohistochemistry fluorescent staining method (TUNEL) was performed to indicate the mechanism of cell death and the fragmented DNA band pattern produced was obtained for verification. Compared to Azadirachta sp. and Typhonium sp., the antiproliferative activity of Brucea sp. extract was the most potent on both KB and ORL-48 cells with IC(50) of 24.37 ± 1.75 and 6.67 ± 1.15 µg/mL, respectively. Signs of cell attrition were observed 24 hr after treatment. Green fluorescent spots indicating cell death by apoptosis were observed in images of both cells following treatment with all the three extracts. DNA fragments harvested from Brucea-treated cells produced bands in a ladder pattern suggesting the apoptotic effect of the extract. It is thus concluded that Brucea sp. extract exhibited cytotoxic activity on ORL-48 cells and their action mechanism is via apoptosis.
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spelling pubmed-41347912014-08-21 Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48 Majid, Mohd Zabidi Mohamad Zaini, Zuraiza Abdul Razak, Fathilah ScientificWorldJournal Research Article Brucea javanica, Azadirachta indica, and Typhonium flagelliforme are medicinal plants commonly used to treat conditions associated with tumour formation. This study aimed to determine the antiproliferative activity of these plants extracts on KB and ORL-48 oral cancer cell lines and to suggest their mode of cell death. The concentration producing 50% cell inhibition (IC(50)) was determined and the activity was examined under an inverted microscope. Immunohistochemistry fluorescent staining method (TUNEL) was performed to indicate the mechanism of cell death and the fragmented DNA band pattern produced was obtained for verification. Compared to Azadirachta sp. and Typhonium sp., the antiproliferative activity of Brucea sp. extract was the most potent on both KB and ORL-48 cells with IC(50) of 24.37 ± 1.75 and 6.67 ± 1.15 µg/mL, respectively. Signs of cell attrition were observed 24 hr after treatment. Green fluorescent spots indicating cell death by apoptosis were observed in images of both cells following treatment with all the three extracts. DNA fragments harvested from Brucea-treated cells produced bands in a ladder pattern suggesting the apoptotic effect of the extract. It is thus concluded that Brucea sp. extract exhibited cytotoxic activity on ORL-48 cells and their action mechanism is via apoptosis. Hindawi Publishing Corporation 2014 2014-07-24 /pmc/articles/PMC4134791/ /pubmed/25147833 http://dx.doi.org/10.1155/2014/125353 Text en Copyright © 2014 Mohd Zabidi Majid et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Majid, Mohd Zabidi
Mohamad Zaini, Zuraiza
Abdul Razak, Fathilah
Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
title Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
title_full Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
title_fullStr Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
title_full_unstemmed Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
title_short Apoptosis-Inducing Effect of Three Medicinal Plants on Oral Cancer Cells KB and ORL-48
title_sort apoptosis-inducing effect of three medicinal plants on oral cancer cells kb and orl-48
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4134791/
https://www.ncbi.nlm.nih.gov/pubmed/25147833
http://dx.doi.org/10.1155/2014/125353
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