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Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells

OBJECTIVES: To investigate the effect of interleukin (IL)-1β on matrix metalloproteinase (MMP)-9 expression in cochlea and regulation of IL-1β-mediated MMP-9 expression by dexamethasone and the molecular and signaling mechanisms involved. METHODS: House ear institute-organ of Corti 1 (HEI-OC1) cells...

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Autores principales: Nam, Sung-Il, Kwon, Taeg-Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Otorhinolaryngology-Head and Neck Surgery 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4135152/
https://www.ncbi.nlm.nih.gov/pubmed/25177432
http://dx.doi.org/10.3342/ceo.2014.7.3.175
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author Nam, Sung-Il
Kwon, Taeg-Kyu
author_facet Nam, Sung-Il
Kwon, Taeg-Kyu
author_sort Nam, Sung-Il
collection PubMed
description OBJECTIVES: To investigate the effect of interleukin (IL)-1β on matrix metalloproteinase (MMP)-9 expression in cochlea and regulation of IL-1β-mediated MMP-9 expression by dexamethasone and the molecular and signaling mechanisms involved. METHODS: House ear institute-organ of Corti 1 (HEI-OC1) cells were used and exposed to IL-1β with/without dexamethasone. Glucocorticoid receptor antagonist, RU486, was used to see the role of dexamethasone. PD98059 (an extracellular signal-regulated kinases [ERKs] inhibitor), SB203580 (a p38 mitogen-activated protein kinases [MAPK] inhibitor), SP600125 (a c-Jun N-terminal kinase [JNK] inhibitor) were also used to see the role of MAPKs signaling pathway(s) in IL-1β-induced MMP-9 expression in HEI-OC1 cells. Reverse transcription-polymerase chain reaction and gelatin zymography were used to measure mRNA expression level of MMP-9 and activity of MMP-9, respectively. RESULTS: Treatment with IL-1β-induced the expression of MMP-9 in a dose- and time-dependent manner. IL-1β (1 ng/mL)-induced MMP-9 expression was inhibited by dexamethasone. Interestingly, p38 MAPK inhibitor, SB203580, significantly inhibited IL-1β-induced MMP-9 mRNA and MMP-9 activity. However, inhibition of JNKs and ERKs had no effect on the IL-1β-induced MMP-9 expression. CONCLUSION: These results suggest that the pro-inflammatory cytokine IL-1β strongly induces MMP-9 expression via activation of p38 MAPK signaling pathway in HEI-OC1 cells and the induction was inhibited by dexamethasone.
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spelling pubmed-41351522014-09-01 Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells Nam, Sung-Il Kwon, Taeg-Kyu Clin Exp Otorhinolaryngol Original Article OBJECTIVES: To investigate the effect of interleukin (IL)-1β on matrix metalloproteinase (MMP)-9 expression in cochlea and regulation of IL-1β-mediated MMP-9 expression by dexamethasone and the molecular and signaling mechanisms involved. METHODS: House ear institute-organ of Corti 1 (HEI-OC1) cells were used and exposed to IL-1β with/without dexamethasone. Glucocorticoid receptor antagonist, RU486, was used to see the role of dexamethasone. PD98059 (an extracellular signal-regulated kinases [ERKs] inhibitor), SB203580 (a p38 mitogen-activated protein kinases [MAPK] inhibitor), SP600125 (a c-Jun N-terminal kinase [JNK] inhibitor) were also used to see the role of MAPKs signaling pathway(s) in IL-1β-induced MMP-9 expression in HEI-OC1 cells. Reverse transcription-polymerase chain reaction and gelatin zymography were used to measure mRNA expression level of MMP-9 and activity of MMP-9, respectively. RESULTS: Treatment with IL-1β-induced the expression of MMP-9 in a dose- and time-dependent manner. IL-1β (1 ng/mL)-induced MMP-9 expression was inhibited by dexamethasone. Interestingly, p38 MAPK inhibitor, SB203580, significantly inhibited IL-1β-induced MMP-9 mRNA and MMP-9 activity. However, inhibition of JNKs and ERKs had no effect on the IL-1β-induced MMP-9 expression. CONCLUSION: These results suggest that the pro-inflammatory cytokine IL-1β strongly induces MMP-9 expression via activation of p38 MAPK signaling pathway in HEI-OC1 cells and the induction was inhibited by dexamethasone. Korean Society of Otorhinolaryngology-Head and Neck Surgery 2014-09 2014-08-01 /pmc/articles/PMC4135152/ /pubmed/25177432 http://dx.doi.org/10.3342/ceo.2014.7.3.175 Text en Copyright © 2014 by Korean Society of Otorhinolaryngology-Head and Neck Surgery. http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Nam, Sung-Il
Kwon, Taeg-Kyu
Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells
title Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells
title_full Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells
title_fullStr Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells
title_full_unstemmed Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells
title_short Dexamethasone Inhibits Interleukin-1β-Induced Matrix Metalloproteinase-9 Expression in Cochlear Cells
title_sort dexamethasone inhibits interleukin-1β-induced matrix metalloproteinase-9 expression in cochlear cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4135152/
https://www.ncbi.nlm.nih.gov/pubmed/25177432
http://dx.doi.org/10.3342/ceo.2014.7.3.175
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