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Mechanistic Insight with HBCH(2)CoA as a Probe to Polyhydroxybutyrate (PHB) Synthases
[Image: see text] Polyhydroxybutyrate (PHB) synthases catalyze the polymerization of 3-(R)-hydroxybutyrate coenzyme A (HBCoA) to produce polyoxoesters of 1–2 MDa. A substrate analogue HBCH(2)CoA, in which the S in HBCoA is replaced with a CH(2) group, was synthesized in 13 steps using a chemoenzymat...
Autores principales: | Zhang, Wei, Shrestha, Ruben, Buckley, Rachael M., Jewell, Jamie, Bossmann, Stefan H., Stubbe, JoAnne, Li, Ping |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136709/ https://www.ncbi.nlm.nih.gov/pubmed/24896226 http://dx.doi.org/10.1021/cb5002735 |
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