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Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice
The effect of pyridostigmine (PYR) - an acetylcholinesterase inhibitor - on hemodynamics and cardiac autonomic control, was never studied in conscious myocardial infarcted mice. Telemetry transmitters were implanted into the carotid artery under isoflurane anesthesia. Seven to ten days after recover...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136726/ https://www.ncbi.nlm.nih.gov/pubmed/25133392 http://dx.doi.org/10.1371/journal.pone.0104476 |
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author | Durand, Marina T. Becari, Christiane de Oliveira, Mauro do Carmo, Jussara M. Aguiar Silva, Carlos Alberto Prado, Cibele M. Fazan, Rubens Salgado, Helio C. |
author_facet | Durand, Marina T. Becari, Christiane de Oliveira, Mauro do Carmo, Jussara M. Aguiar Silva, Carlos Alberto Prado, Cibele M. Fazan, Rubens Salgado, Helio C. |
author_sort | Durand, Marina T. |
collection | PubMed |
description | The effect of pyridostigmine (PYR) - an acetylcholinesterase inhibitor - on hemodynamics and cardiac autonomic control, was never studied in conscious myocardial infarcted mice. Telemetry transmitters were implanted into the carotid artery under isoflurane anesthesia. Seven to ten days after recovery from the surgery, basal arterial pressure and heart rate were recorded, while parasympathetic and sympathetic tone (ΔHR) was evaluated by means of methyl atropine and propranolol. After the basal hemodynamic recording the mice were subjected to left coronary artery ligation for producing myocardial infarction (MI), or sham operation, and implantation of minipumps filled with PYR or saline. Separate groups of anesthetized (isoflurane) mice previously (4 weeks) subjected to MI, or sham coronary artery ligation, were submitted to cardiac function examination. The mice exhibited an infarct length of approximately 12%, no change in arterial pressure and increased heart rate only in the 1st week after MI. Vagal tone decreased in the 1(st) week, while the sympathetic tone was increased in the 1(st) and 4(th) week after MI. PYR prevented the increase in heart rate but did not affect the arterial pressure. Moreover, PYR prevented the increase in sympathetic tone throughout the 4 weeks. Concerning the parasympathetic tone, PYR not only impaired its attenuation in the 1(st) week, but enhanced it in the 4(th) week. MI decreased ejection fraction and increased diastolic and systolic volume. Therefore, the pharmacological increase of peripheral acetylcholine availability by means of PYR prevented tachycardia, increased parasympathetic and decreased sympathetic tone after MI in mice. |
format | Online Article Text |
id | pubmed-4136726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41367262014-08-20 Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice Durand, Marina T. Becari, Christiane de Oliveira, Mauro do Carmo, Jussara M. Aguiar Silva, Carlos Alberto Prado, Cibele M. Fazan, Rubens Salgado, Helio C. PLoS One Research Article The effect of pyridostigmine (PYR) - an acetylcholinesterase inhibitor - on hemodynamics and cardiac autonomic control, was never studied in conscious myocardial infarcted mice. Telemetry transmitters were implanted into the carotid artery under isoflurane anesthesia. Seven to ten days after recovery from the surgery, basal arterial pressure and heart rate were recorded, while parasympathetic and sympathetic tone (ΔHR) was evaluated by means of methyl atropine and propranolol. After the basal hemodynamic recording the mice were subjected to left coronary artery ligation for producing myocardial infarction (MI), or sham operation, and implantation of minipumps filled with PYR or saline. Separate groups of anesthetized (isoflurane) mice previously (4 weeks) subjected to MI, or sham coronary artery ligation, were submitted to cardiac function examination. The mice exhibited an infarct length of approximately 12%, no change in arterial pressure and increased heart rate only in the 1st week after MI. Vagal tone decreased in the 1(st) week, while the sympathetic tone was increased in the 1(st) and 4(th) week after MI. PYR prevented the increase in heart rate but did not affect the arterial pressure. Moreover, PYR prevented the increase in sympathetic tone throughout the 4 weeks. Concerning the parasympathetic tone, PYR not only impaired its attenuation in the 1(st) week, but enhanced it in the 4(th) week. MI decreased ejection fraction and increased diastolic and systolic volume. Therefore, the pharmacological increase of peripheral acetylcholine availability by means of PYR prevented tachycardia, increased parasympathetic and decreased sympathetic tone after MI in mice. Public Library of Science 2014-08-18 /pmc/articles/PMC4136726/ /pubmed/25133392 http://dx.doi.org/10.1371/journal.pone.0104476 Text en © 2014 Durand et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Durand, Marina T. Becari, Christiane de Oliveira, Mauro do Carmo, Jussara M. Aguiar Silva, Carlos Alberto Prado, Cibele M. Fazan, Rubens Salgado, Helio C. Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice |
title | Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice |
title_full | Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice |
title_fullStr | Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice |
title_full_unstemmed | Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice |
title_short | Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice |
title_sort | pyridostigmine restores cardiac autonomic balance after small myocardial infarction in mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136726/ https://www.ncbi.nlm.nih.gov/pubmed/25133392 http://dx.doi.org/10.1371/journal.pone.0104476 |
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