Cargando…
Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season
BACKGROUND: Switchgrass (Panicum virgatum L.) is being developed as a bioenergy crop for many temperate regions of the world. One way to increase biomass yields is to move southern adapted lowland cultivars to more northern latitudes. However, many southerly adapted switchgrass germplasm can suffer...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136849/ https://www.ncbi.nlm.nih.gov/pubmed/25133804 http://dx.doi.org/10.1371/journal.pone.0105138 |
_version_ | 1782331038241914880 |
---|---|
author | Palmer, Nathan A. Saathoff, Aaron J. Tobias, Christian M. Twigg, Paul Xia, Yuannan Vogel, Kenneth P. Madhavan, Soundararajan Sattler, Scott E. Sarath, Gautam |
author_facet | Palmer, Nathan A. Saathoff, Aaron J. Tobias, Christian M. Twigg, Paul Xia, Yuannan Vogel, Kenneth P. Madhavan, Soundararajan Sattler, Scott E. Sarath, Gautam |
author_sort | Palmer, Nathan A. |
collection | PubMed |
description | BACKGROUND: Switchgrass (Panicum virgatum L.) is being developed as a bioenergy crop for many temperate regions of the world. One way to increase biomass yields is to move southern adapted lowland cultivars to more northern latitudes. However, many southerly adapted switchgrass germplasm can suffer significant winter kill in northerly climes. MATERIALS AND METHODS: Here, we have applied next-generation sequencing in combination with biochemical analyses to query the metabolism of crowns and rhizomes obtained from two contrasting switchgrass cultivars. Crowns and rhizomes from field-grown lowland (cv Kanlow) and upland (cv Summer) switchgrass cultivars were collected from three randomly selected post-flowering plants. Summer plants were senescing, whereas Kanlow plants were not at this harvest date. RESULTS: Principal component analysis (PCA) differentiated between both the Summer and Kanlow transcriptomes and metabolomes. Significant differences in transcript abundances were detected for 8,050 genes, including transcription factors such as WRKYs and those associated with phenylpropanoid biosynthesis. Gene-set enrichment analyses showed that a number of pathways were differentially up-regulated in the two populations. For both populations, protein levels and enzyme activities agreed well with transcript abundances for genes involved in the phenylpropanoid pathway that were up-regulated in Kanlow crowns and rhizomes. The combination of these datasets suggests that dormancy-related mechanisms had been triggered in the crowns and rhizomes of the Summer plants, whereas the crowns and rhizomes of Kanlow plants had yet to enter dormancy. CONCLUSIONS: Delayed establishment of dormancy at more northerly latitudes could be one factor that reduces winter-survival in the high-yielding Kanlow plants. Understanding the cellular signatures that accompany the transition to dormancy can be used in the future to select plants with improved winter hardiness. |
format | Online Article Text |
id | pubmed-4136849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41368492014-08-20 Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season Palmer, Nathan A. Saathoff, Aaron J. Tobias, Christian M. Twigg, Paul Xia, Yuannan Vogel, Kenneth P. Madhavan, Soundararajan Sattler, Scott E. Sarath, Gautam PLoS One Research Article BACKGROUND: Switchgrass (Panicum virgatum L.) is being developed as a bioenergy crop for many temperate regions of the world. One way to increase biomass yields is to move southern adapted lowland cultivars to more northern latitudes. However, many southerly adapted switchgrass germplasm can suffer significant winter kill in northerly climes. MATERIALS AND METHODS: Here, we have applied next-generation sequencing in combination with biochemical analyses to query the metabolism of crowns and rhizomes obtained from two contrasting switchgrass cultivars. Crowns and rhizomes from field-grown lowland (cv Kanlow) and upland (cv Summer) switchgrass cultivars were collected from three randomly selected post-flowering plants. Summer plants were senescing, whereas Kanlow plants were not at this harvest date. RESULTS: Principal component analysis (PCA) differentiated between both the Summer and Kanlow transcriptomes and metabolomes. Significant differences in transcript abundances were detected for 8,050 genes, including transcription factors such as WRKYs and those associated with phenylpropanoid biosynthesis. Gene-set enrichment analyses showed that a number of pathways were differentially up-regulated in the two populations. For both populations, protein levels and enzyme activities agreed well with transcript abundances for genes involved in the phenylpropanoid pathway that were up-regulated in Kanlow crowns and rhizomes. The combination of these datasets suggests that dormancy-related mechanisms had been triggered in the crowns and rhizomes of the Summer plants, whereas the crowns and rhizomes of Kanlow plants had yet to enter dormancy. CONCLUSIONS: Delayed establishment of dormancy at more northerly latitudes could be one factor that reduces winter-survival in the high-yielding Kanlow plants. Understanding the cellular signatures that accompany the transition to dormancy can be used in the future to select plants with improved winter hardiness. Public Library of Science 2014-08-18 /pmc/articles/PMC4136849/ /pubmed/25133804 http://dx.doi.org/10.1371/journal.pone.0105138 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Palmer, Nathan A. Saathoff, Aaron J. Tobias, Christian M. Twigg, Paul Xia, Yuannan Vogel, Kenneth P. Madhavan, Soundararajan Sattler, Scott E. Sarath, Gautam Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season |
title | Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season |
title_full | Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season |
title_fullStr | Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season |
title_full_unstemmed | Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season |
title_short | Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season |
title_sort | contrasting metabolism in perenniating structures of upland and lowland switchgrass plants late in the growing season |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136849/ https://www.ncbi.nlm.nih.gov/pubmed/25133804 http://dx.doi.org/10.1371/journal.pone.0105138 |
work_keys_str_mv | AT palmernathana contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT saathoffaaronj contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT tobiaschristianm contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT twiggpaul contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT xiayuannan contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT vogelkennethp contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT madhavansoundararajan contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT sattlerscotte contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason AT sarathgautam contrastingmetabolisminperenniatingstructuresofuplandandlowlandswitchgrassplantslateinthegrowingseason |