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MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation

Spontaneous immune tolerance in mouse liver transplantation has always been a hotspot in transplantation-immune research. Recent studies revealed that regulatory T cells (Tregs), hepatic satellite cells and Kupffer cells play a potential role in spontaneous immune tolerance, however the precise mech...

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Autores principales: Wang, Yan, Tian, Yang, Ding, Yuan, Wang, Jingcheng, Yan, Sheng, Zhou, Lin, Xie, Haiyang, Chen, Hui, Li, Hui, Zhang, Jinhua, Zhao, Jiacong, Zheng, Shusen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136864/
https://www.ncbi.nlm.nih.gov/pubmed/25133393
http://dx.doi.org/10.1371/journal.pone.0105096
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author Wang, Yan
Tian, Yang
Ding, Yuan
Wang, Jingcheng
Yan, Sheng
Zhou, Lin
Xie, Haiyang
Chen, Hui
Li, Hui
Zhang, Jinhua
Zhao, Jiacong
Zheng, Shusen
author_facet Wang, Yan
Tian, Yang
Ding, Yuan
Wang, Jingcheng
Yan, Sheng
Zhou, Lin
Xie, Haiyang
Chen, Hui
Li, Hui
Zhang, Jinhua
Zhao, Jiacong
Zheng, Shusen
author_sort Wang, Yan
collection PubMed
description Spontaneous immune tolerance in mouse liver transplantation has always been a hotspot in transplantation-immune research. Recent studies revealed that regulatory T cells (Tregs), hepatic satellite cells and Kupffer cells play a potential role in spontaneous immune tolerance, however the precise mechanism of spontaneous immune tolerance is still undefined. By using Microarray Chips, we investigated different immune regulatory factors to decipher critical mechanisms of spontaneous tolerance after mouse liver transplantation. Allogeneic (C57BL/6-C3H) and syngeneic (C3H-C3H) liver transplantation were performed by 6-8 weeks old male C57BL/6 and C3H mice. Graft samples (N = 4 each group) were collected from 8 weeks post-operation mice. 11 differentially expressed miRNAs in allogeneic grafts (Allografts) vs. syngeneic grafts (Syngrafts) were identified using Agilent Mouse miRNA Chips. It was revealed that 185 genes were modified by the 11 miRNAs, furthermore, within the 185 target genes, 11 of them were tightly correlated with immune regulation after Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Genbank data cross-comparison. Verified by real-time PCR and western blot, our results indicated that mRNA expression levels of IL-6 and TAB2 were respectively down regulated following miR-142-3p and miR-155 augment. In addition, increased miR-152 just silenced mRNA of CaMK II and down-regulated translation of CaMK II in tolerated liver grafts, which may play a critical role in immune regulation and spontaneous tolerance induction of mouse liver transplantation.
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spelling pubmed-41368642014-08-20 MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation Wang, Yan Tian, Yang Ding, Yuan Wang, Jingcheng Yan, Sheng Zhou, Lin Xie, Haiyang Chen, Hui Li, Hui Zhang, Jinhua Zhao, Jiacong Zheng, Shusen PLoS One Research Article Spontaneous immune tolerance in mouse liver transplantation has always been a hotspot in transplantation-immune research. Recent studies revealed that regulatory T cells (Tregs), hepatic satellite cells and Kupffer cells play a potential role in spontaneous immune tolerance, however the precise mechanism of spontaneous immune tolerance is still undefined. By using Microarray Chips, we investigated different immune regulatory factors to decipher critical mechanisms of spontaneous tolerance after mouse liver transplantation. Allogeneic (C57BL/6-C3H) and syngeneic (C3H-C3H) liver transplantation were performed by 6-8 weeks old male C57BL/6 and C3H mice. Graft samples (N = 4 each group) were collected from 8 weeks post-operation mice. 11 differentially expressed miRNAs in allogeneic grafts (Allografts) vs. syngeneic grafts (Syngrafts) were identified using Agilent Mouse miRNA Chips. It was revealed that 185 genes were modified by the 11 miRNAs, furthermore, within the 185 target genes, 11 of them were tightly correlated with immune regulation after Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Genbank data cross-comparison. Verified by real-time PCR and western blot, our results indicated that mRNA expression levels of IL-6 and TAB2 were respectively down regulated following miR-142-3p and miR-155 augment. In addition, increased miR-152 just silenced mRNA of CaMK II and down-regulated translation of CaMK II in tolerated liver grafts, which may play a critical role in immune regulation and spontaneous tolerance induction of mouse liver transplantation. Public Library of Science 2014-08-18 /pmc/articles/PMC4136864/ /pubmed/25133393 http://dx.doi.org/10.1371/journal.pone.0105096 Text en © 2014 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Yan
Tian, Yang
Ding, Yuan
Wang, Jingcheng
Yan, Sheng
Zhou, Lin
Xie, Haiyang
Chen, Hui
Li, Hui
Zhang, Jinhua
Zhao, Jiacong
Zheng, Shusen
MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation
title MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation
title_full MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation
title_fullStr MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation
title_full_unstemmed MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation
title_short MiR-152 May Silence Translation of CaMK II and Induce Spontaneous Immune Tolerance in Mouse Liver Transplantation
title_sort mir-152 may silence translation of camk ii and induce spontaneous immune tolerance in mouse liver transplantation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4136864/
https://www.ncbi.nlm.nih.gov/pubmed/25133393
http://dx.doi.org/10.1371/journal.pone.0105096
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