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Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE
The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Pub. Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137407/ https://www.ncbi.nlm.nih.gov/pubmed/25088546 http://dx.doi.org/10.1038/ncomms5521 |
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author | Wisedchaisri, Goragot Park, Min-Sun Iadanza, Matthew G. Zheng, Hongjin Gonen, Tamir |
author_facet | Wisedchaisri, Goragot Park, Min-Sun Iadanza, Matthew G. Zheng, Hongjin Gonen, Tamir |
author_sort | Wisedchaisri, Goragot |
collection | PubMed |
description | The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformations, including both the outward and inward-facing states. Here we report the crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the rocker-switch movement of the N-domain against the C-domain during the transport cycle. Using molecular dynamics simulation, and functional transport assays, we describe the movement of XylE that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133. This study addresses the structural basis for proton-coupled substrate transport and release mechanism for the sugar porter family of proteins. |
format | Online Article Text |
id | pubmed-4137407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Pub. Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-41374072015-02-04 Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE Wisedchaisri, Goragot Park, Min-Sun Iadanza, Matthew G. Zheng, Hongjin Gonen, Tamir Nat Commun Article The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformations, including both the outward and inward-facing states. Here we report the crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the rocker-switch movement of the N-domain against the C-domain during the transport cycle. Using molecular dynamics simulation, and functional transport assays, we describe the movement of XylE that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133. This study addresses the structural basis for proton-coupled substrate transport and release mechanism for the sugar porter family of proteins. Nature Pub. Group 2014-08-04 /pmc/articles/PMC4137407/ /pubmed/25088546 http://dx.doi.org/10.1038/ncomms5521 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/ |
spellingShingle | Article Wisedchaisri, Goragot Park, Min-Sun Iadanza, Matthew G. Zheng, Hongjin Gonen, Tamir Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE |
title | Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE |
title_full | Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE |
title_fullStr | Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE |
title_full_unstemmed | Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE |
title_short | Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE |
title_sort | proton-coupled sugar transport in the prototypical major facilitator superfamily protein xyle |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137407/ https://www.ncbi.nlm.nih.gov/pubmed/25088546 http://dx.doi.org/10.1038/ncomms5521 |
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