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Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE

The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformati...

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Autores principales: Wisedchaisri, Goragot, Park, Min-Sun, Iadanza, Matthew G., Zheng, Hongjin, Gonen, Tamir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137407/
https://www.ncbi.nlm.nih.gov/pubmed/25088546
http://dx.doi.org/10.1038/ncomms5521
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author Wisedchaisri, Goragot
Park, Min-Sun
Iadanza, Matthew G.
Zheng, Hongjin
Gonen, Tamir
author_facet Wisedchaisri, Goragot
Park, Min-Sun
Iadanza, Matthew G.
Zheng, Hongjin
Gonen, Tamir
author_sort Wisedchaisri, Goragot
collection PubMed
description The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformations, including both the outward and inward-facing states. Here we report the crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the rocker-switch movement of the N-domain against the C-domain during the transport cycle. Using molecular dynamics simulation, and functional transport assays, we describe the movement of XylE that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133. This study addresses the structural basis for proton-coupled substrate transport and release mechanism for the sugar porter family of proteins.
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spelling pubmed-41374072015-02-04 Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE Wisedchaisri, Goragot Park, Min-Sun Iadanza, Matthew G. Zheng, Hongjin Gonen, Tamir Nat Commun Article The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformations, including both the outward and inward-facing states. Here we report the crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the rocker-switch movement of the N-domain against the C-domain during the transport cycle. Using molecular dynamics simulation, and functional transport assays, we describe the movement of XylE that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133. This study addresses the structural basis for proton-coupled substrate transport and release mechanism for the sugar porter family of proteins. Nature Pub. Group 2014-08-04 /pmc/articles/PMC4137407/ /pubmed/25088546 http://dx.doi.org/10.1038/ncomms5521 Text en Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Wisedchaisri, Goragot
Park, Min-Sun
Iadanza, Matthew G.
Zheng, Hongjin
Gonen, Tamir
Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE
title Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE
title_full Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE
title_fullStr Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE
title_full_unstemmed Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE
title_short Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE
title_sort proton-coupled sugar transport in the prototypical major facilitator superfamily protein xyle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137407/
https://www.ncbi.nlm.nih.gov/pubmed/25088546
http://dx.doi.org/10.1038/ncomms5521
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