Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis

Previous studies showed that hydrolysates of β-lactoglobulin (BLG) prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV) activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system....

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Autores principales: Shigemori, Suguru, Oshiro, Kazushi, Wang, Pengfei, Yamamoto, Yoshinari, Wang, Yeqin, Sato, Takashi, Uyeno, Yutaka, Shimosato, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137494/
https://www.ncbi.nlm.nih.gov/pubmed/25157356
http://dx.doi.org/10.1155/2014/393598
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author Shigemori, Suguru
Oshiro, Kazushi
Wang, Pengfei
Yamamoto, Yoshinari
Wang, Yeqin
Sato, Takashi
Uyeno, Yutaka
Shimosato, Takeshi
author_facet Shigemori, Suguru
Oshiro, Kazushi
Wang, Pengfei
Yamamoto, Yoshinari
Wang, Yeqin
Sato, Takashi
Uyeno, Yutaka
Shimosato, Takeshi
author_sort Shigemori, Suguru
collection PubMed
description Previous studies showed that hydrolysates of β-lactoglobulin (BLG) prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV) activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system. Interestingly, trypsin-digested recombinant BLG from L. lactis inhibited DPP-IV activity, suggesting that BLG-secreting L. lactis may be useful in the treatment of type 2 diabetes mellitus.
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spelling pubmed-41374942014-08-25 Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis Shigemori, Suguru Oshiro, Kazushi Wang, Pengfei Yamamoto, Yoshinari Wang, Yeqin Sato, Takashi Uyeno, Yutaka Shimosato, Takeshi Biomed Res Int Research Article Previous studies showed that hydrolysates of β-lactoglobulin (BLG) prepared using gastrointestinal proteases strongly inhibit dipeptidyl peptidase-IV (DPP-IV) activity in vitro. In this study, we developed a BLG-secreting Lactococcus lactis strain as a delivery vehicle and in situ expression system. Interestingly, trypsin-digested recombinant BLG from L. lactis inhibited DPP-IV activity, suggesting that BLG-secreting L. lactis may be useful in the treatment of type 2 diabetes mellitus. Hindawi Publishing Corporation 2014 2014-08-03 /pmc/articles/PMC4137494/ /pubmed/25157356 http://dx.doi.org/10.1155/2014/393598 Text en Copyright © 2014 Suguru Shigemori et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shigemori, Suguru
Oshiro, Kazushi
Wang, Pengfei
Yamamoto, Yoshinari
Wang, Yeqin
Sato, Takashi
Uyeno, Yutaka
Shimosato, Takeshi
Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
title Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
title_full Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
title_fullStr Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
title_full_unstemmed Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
title_short Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
title_sort generation of dipeptidyl peptidase-iv-inhibiting peptides from β-lactoglobulin secreted by lactococcus lactis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137494/
https://www.ncbi.nlm.nih.gov/pubmed/25157356
http://dx.doi.org/10.1155/2014/393598
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