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Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats

Diabetes mellitus is associated with dysregulation of adipose tissue metabolism and increased level of serum lipids. In our previous work we found that Securigera securidaca decreases cholesterol level in blood of diabetic rats. The present study was carried out to further investigate the effects of...

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Autores principales: Ghorbani, Ahmad, Moradi Marjaneh, Reyhaneh, Rajaei, Ziba, Hadjzadeh, Mousa-Al-Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137550/
https://www.ncbi.nlm.nih.gov/pubmed/25161769
http://dx.doi.org/10.1155/2014/582106
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author Ghorbani, Ahmad
Moradi Marjaneh, Reyhaneh
Rajaei, Ziba
Hadjzadeh, Mousa-Al-Reza
author_facet Ghorbani, Ahmad
Moradi Marjaneh, Reyhaneh
Rajaei, Ziba
Hadjzadeh, Mousa-Al-Reza
author_sort Ghorbani, Ahmad
collection PubMed
description Diabetes mellitus is associated with dysregulation of adipose tissue metabolism and increased level of serum lipids. In our previous work we found that Securigera securidaca decreases cholesterol level in blood of diabetic rats. The present study was carried out to further investigate the effects of this plant on lipid metabolism, lipolysis, and adipogenesis, in diabetic rats. Female Wistar rats were rendered diabetic by intraperitoneal injection of streptozotocin. Retroperitoneal adipose tissue was removed from diabetic animals after seven days of streptozotocin injection. Effect of hydroalcoholic extract of S. securidaca seeds (100–800 μg/mL) on adipose tissue lipolysis was evaluated in ex vivo condition. Also, to evaluate adipogenesis, preadipocytes were isolated from adipose tissue and differentiated to adipocytes in the presence of the extract. The extract at concentration of 800 μg/mL decreased both basal and catecholamine-stimulated lipolysis (P < 0.05). Incubation of differentiating preadipocytes with 800 μg/mL of S. securidaca extract decreased intracellular lipid droplet accumulation as evaluated with Oil Red O staining (P < 0.001). The extract even at high concentrations had no effect on viability of preadipocytes. In conclusion, S. securidaca decreases lipolysis and adipogenesis without cytotoxicity, which makes it a good candidate for management of dyslipidemia and reduction of cardiovascular risks in diabetes.
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spelling pubmed-41375502014-08-26 Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats Ghorbani, Ahmad Moradi Marjaneh, Reyhaneh Rajaei, Ziba Hadjzadeh, Mousa-Al-Reza Cholesterol Research Article Diabetes mellitus is associated with dysregulation of adipose tissue metabolism and increased level of serum lipids. In our previous work we found that Securigera securidaca decreases cholesterol level in blood of diabetic rats. The present study was carried out to further investigate the effects of this plant on lipid metabolism, lipolysis, and adipogenesis, in diabetic rats. Female Wistar rats were rendered diabetic by intraperitoneal injection of streptozotocin. Retroperitoneal adipose tissue was removed from diabetic animals after seven days of streptozotocin injection. Effect of hydroalcoholic extract of S. securidaca seeds (100–800 μg/mL) on adipose tissue lipolysis was evaluated in ex vivo condition. Also, to evaluate adipogenesis, preadipocytes were isolated from adipose tissue and differentiated to adipocytes in the presence of the extract. The extract at concentration of 800 μg/mL decreased both basal and catecholamine-stimulated lipolysis (P < 0.05). Incubation of differentiating preadipocytes with 800 μg/mL of S. securidaca extract decreased intracellular lipid droplet accumulation as evaluated with Oil Red O staining (P < 0.001). The extract even at high concentrations had no effect on viability of preadipocytes. In conclusion, S. securidaca decreases lipolysis and adipogenesis without cytotoxicity, which makes it a good candidate for management of dyslipidemia and reduction of cardiovascular risks in diabetes. Hindawi Publishing Corporation 2014 2014-08-03 /pmc/articles/PMC4137550/ /pubmed/25161769 http://dx.doi.org/10.1155/2014/582106 Text en Copyright © 2014 Ahmad Ghorbani et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ghorbani, Ahmad
Moradi Marjaneh, Reyhaneh
Rajaei, Ziba
Hadjzadeh, Mousa-Al-Reza
Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats
title Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats
title_full Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats
title_fullStr Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats
title_full_unstemmed Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats
title_short Effects of Securigera securidaca Extract on Lipolysis and Adipogenesis in Diabetic Rats
title_sort effects of securigera securidaca extract on lipolysis and adipogenesis in diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137550/
https://www.ncbi.nlm.nih.gov/pubmed/25161769
http://dx.doi.org/10.1155/2014/582106
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