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Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining

Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based as...

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Autores principales: Oliveira, Tatiane, Figueiredo, Camila A., Brito, Carlos, Stavroullakis, Alexander, Prakki, Anuradha, Da Silva Velozo, Eudes, Nogueira-Filho, Getulio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137552/
https://www.ncbi.nlm.nih.gov/pubmed/25221602
http://dx.doi.org/10.1155/2014/535789
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author Oliveira, Tatiane
Figueiredo, Camila A.
Brito, Carlos
Stavroullakis, Alexander
Prakki, Anuradha
Da Silva Velozo, Eudes
Nogueira-Filho, Getulio
author_facet Oliveira, Tatiane
Figueiredo, Camila A.
Brito, Carlos
Stavroullakis, Alexander
Prakki, Anuradha
Da Silva Velozo, Eudes
Nogueira-Filho, Getulio
author_sort Oliveira, Tatiane
collection PubMed
description Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO(2). Resazurin reduction and total protein content assays were used to detect cell viability. AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells.
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spelling pubmed-41375522014-09-14 Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining Oliveira, Tatiane Figueiredo, Camila A. Brito, Carlos Stavroullakis, Alexander Prakki, Anuradha Da Silva Velozo, Eudes Nogueira-Filho, Getulio Int J Cell Biol Research Article Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO(2). Resazurin reduction and total protein content assays were used to detect cell viability. AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells. Hindawi Publishing Corporation 2014 2014-08-03 /pmc/articles/PMC4137552/ /pubmed/25221602 http://dx.doi.org/10.1155/2014/535789 Text en Copyright © 2014 Tatiane Oliveira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Oliveira, Tatiane
Figueiredo, Camila A.
Brito, Carlos
Stavroullakis, Alexander
Prakki, Anuradha
Da Silva Velozo, Eudes
Nogueira-Filho, Getulio
Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
title Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
title_full Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
title_fullStr Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
title_full_unstemmed Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
title_short Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
title_sort effect of allium cepa l. on lipopolysaccharide-stimulated osteoclast precursor cell viability, count, and morphology using 4′,6-diamidino-2-phenylindole-staining
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137552/
https://www.ncbi.nlm.nih.gov/pubmed/25221602
http://dx.doi.org/10.1155/2014/535789
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