Cargando…
Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining
Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based as...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137552/ https://www.ncbi.nlm.nih.gov/pubmed/25221602 http://dx.doi.org/10.1155/2014/535789 |
_version_ | 1782331122166792192 |
---|---|
author | Oliveira, Tatiane Figueiredo, Camila A. Brito, Carlos Stavroullakis, Alexander Prakki, Anuradha Da Silva Velozo, Eudes Nogueira-Filho, Getulio |
author_facet | Oliveira, Tatiane Figueiredo, Camila A. Brito, Carlos Stavroullakis, Alexander Prakki, Anuradha Da Silva Velozo, Eudes Nogueira-Filho, Getulio |
author_sort | Oliveira, Tatiane |
collection | PubMed |
description | Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO(2). Resazurin reduction and total protein content assays were used to detect cell viability. AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells. |
format | Online Article Text |
id | pubmed-4137552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-41375522014-09-14 Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining Oliveira, Tatiane Figueiredo, Camila A. Brito, Carlos Stavroullakis, Alexander Prakki, Anuradha Da Silva Velozo, Eudes Nogueira-Filho, Getulio Int J Cell Biol Research Article Allium cepa L. is known to possess numerous pharmacological properties. Our aim was to examine the in vitro effects of Allium cepa L. extract (AcE) on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells to determine cell viability to other future cell-based assays. Osteoclast precursor cells (RAW 264.7) were stimulated by Pg LPS (1 μg/mL) and E. coli LPS (1 μg/mL) in the presence or absence of different concentrations of AcE (10–1000 μg/mL) for 5 days at 37°C/5% CO(2). Resazurin reduction and total protein content assays were used to detect cell viability. AcE did not affect cell viability. Resazurin reduction assay showed that AcE, at up to 1000 μg/mL, did not significantly affect cell viability and cellular protein levels. Additionally a caspase 3/7 luminescence assay was used to disclose apoptosis and there was no difference in apoptotic activity between tested groups and control group. Fluorescence images stained by DAPI showed no alteration on the morphology and cell counts of LPS-stimulated osteoclast precursor cells with the use of AcE in all tested concentrations when compared to control. These findings suggest that Allium cepa L. extract could be used for in vitro studies on Porphyromonas gingivalis LPS and Escherichia coli LPS-stimulated osteoclast precursor cells. Hindawi Publishing Corporation 2014 2014-08-03 /pmc/articles/PMC4137552/ /pubmed/25221602 http://dx.doi.org/10.1155/2014/535789 Text en Copyright © 2014 Tatiane Oliveira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Oliveira, Tatiane Figueiredo, Camila A. Brito, Carlos Stavroullakis, Alexander Prakki, Anuradha Da Silva Velozo, Eudes Nogueira-Filho, Getulio Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
title | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
title_full | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
title_fullStr | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
title_full_unstemmed | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
title_short | Effect of Allium cepa L. on Lipopolysaccharide-Stimulated Osteoclast Precursor Cell Viability, Count, and Morphology Using 4′,6-Diamidino-2-phenylindole-Staining |
title_sort | effect of allium cepa l. on lipopolysaccharide-stimulated osteoclast precursor cell viability, count, and morphology using 4′,6-diamidino-2-phenylindole-staining |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137552/ https://www.ncbi.nlm.nih.gov/pubmed/25221602 http://dx.doi.org/10.1155/2014/535789 |
work_keys_str_mv | AT oliveiratatiane effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT figueiredocamilaa effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT britocarlos effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT stavroullakisalexander effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT prakkianuradha effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT dasilvavelozoeudes effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining AT nogueirafilhogetulio effectofalliumcepalonlipopolysaccharidestimulatedosteoclastprecursorcellviabilitycountandmorphologyusing46diamidino2phenylindolestaining |