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Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey

OBJECTIVE(S): Ethnopharmacological field surveys demonstrated that many plants, such as Gentiana olivieri, Helichrysum graveolens, Helichrysum plicatum ssp. plicatum, Juniperus oxycedrus ssp. oxycedrus, Juniperus communis var. saxatilis, Viscum album (ssp. album, ssp. austriacum), are used as tradit...

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Autores principales: Orhan, Nilüfer, Hoçbaç, Sanem, Orhan, Didem Deliorman, Asian, Mustafa, Ergun, Fatma
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137942/
https://www.ncbi.nlm.nih.gov/pubmed/25140204
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author Orhan, Nilüfer
Hoçbaç, Sanem
Orhan, Didem Deliorman
Asian, Mustafa
Ergun, Fatma
author_facet Orhan, Nilüfer
Hoçbaç, Sanem
Orhan, Didem Deliorman
Asian, Mustafa
Ergun, Fatma
author_sort Orhan, Nilüfer
collection PubMed
description OBJECTIVE(S): Ethnopharmacological field surveys demonstrated that many plants, such as Gentiana olivieri, Helichrysum graveolens, Helichrysum plicatum ssp. plicatum, Juniperus oxycedrus ssp. oxycedrus, Juniperus communis var. saxatilis, Viscum album (ssp. album, ssp. austriacum), are used as traditional medicine for diabetes in different regions of Anatolia. The present study was designed to evaluate the in vitro antidiabetic effects of some selected plants, tested in animal models recently. MATERIALS AND METHODS: α-glucosidase and α-amylase enzyme inhibitory effects of the plant extracts were investigated and Acarbose was used as a reference drug. Additionally, radical scavenging capacities were determined using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical cation scavenging assay and total phenolic content of the extracts were evaluated using Folin Ciocalteu method. RESULTS: H. graveolens ethanol extract exhibited the highest inhibitory activity (55.7 % ± 2.2) on α-amylase enzyme. Additionally, J. oxycedrus hydro-alcoholic leaf extract had potent α-amylase inhibitory effect, while the hydro-alcoholic extract of J. communis fruit showed the highest α-glucosidase inhibitory activity (IC(50): 4.4 μg/ml). CONCLUSION: Results indicated that, antidiabetic effect of hydro-alcoholic extracts of H. graveolens capitulums, J. communis fruit and J. oxycedrus leaf might arise from inhibition of digestive enzymes.
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spelling pubmed-41379422014-08-19 Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey Orhan, Nilüfer Hoçbaç, Sanem Orhan, Didem Deliorman Asian, Mustafa Ergun, Fatma Iran J Basic Med Sci Original Article OBJECTIVE(S): Ethnopharmacological field surveys demonstrated that many plants, such as Gentiana olivieri, Helichrysum graveolens, Helichrysum plicatum ssp. plicatum, Juniperus oxycedrus ssp. oxycedrus, Juniperus communis var. saxatilis, Viscum album (ssp. album, ssp. austriacum), are used as traditional medicine for diabetes in different regions of Anatolia. The present study was designed to evaluate the in vitro antidiabetic effects of some selected plants, tested in animal models recently. MATERIALS AND METHODS: α-glucosidase and α-amylase enzyme inhibitory effects of the plant extracts were investigated and Acarbose was used as a reference drug. Additionally, radical scavenging capacities were determined using 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ABTS radical cation scavenging assay and total phenolic content of the extracts were evaluated using Folin Ciocalteu method. RESULTS: H. graveolens ethanol extract exhibited the highest inhibitory activity (55.7 % ± 2.2) on α-amylase enzyme. Additionally, J. oxycedrus hydro-alcoholic leaf extract had potent α-amylase inhibitory effect, while the hydro-alcoholic extract of J. communis fruit showed the highest α-glucosidase inhibitory activity (IC(50): 4.4 μg/ml). CONCLUSION: Results indicated that, antidiabetic effect of hydro-alcoholic extracts of H. graveolens capitulums, J. communis fruit and J. oxycedrus leaf might arise from inhibition of digestive enzymes. Mashhad University of Medical Sciences 2014-06 /pmc/articles/PMC4137942/ /pubmed/25140204 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Orhan, Nilüfer
Hoçbaç, Sanem
Orhan, Didem Deliorman
Asian, Mustafa
Ergun, Fatma
Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
title Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
title_full Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
title_fullStr Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
title_full_unstemmed Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
title_short Enzyme inhibitory and radical scavenging effects of some antidiabetic plants of Turkey
title_sort enzyme inhibitory and radical scavenging effects of some antidiabetic plants of turkey
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137942/
https://www.ncbi.nlm.nih.gov/pubmed/25140204
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