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CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters
BACKGROUND: The bacterial genus Salmonella contains thousands of serotypes that infect humans or other hosts, causing mild gastroenteritis to potentially fatal systemic infections in humans. Pathogenically distinct Salmonella serotypes have been classified as individual species or as serological var...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4138082/ https://www.ncbi.nlm.nih.gov/pubmed/25137186 http://dx.doi.org/10.1371/journal.pone.0103388 |
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author | Tang, Le Liu, Wei-Qiao Fang, Xin Sun, Qiang Zhu, Song-Ling Wang, Chun-Xiao Wang, Xiao-Yu Li, Yong-Guo Zhu, Da-Ling Sanderson, Kenneth E. Johnston, Randal N. Liu, Gui-Rong Liu, Shu-Lin |
author_facet | Tang, Le Liu, Wei-Qiao Fang, Xin Sun, Qiang Zhu, Song-Ling Wang, Chun-Xiao Wang, Xiao-Yu Li, Yong-Guo Zhu, Da-Ling Sanderson, Kenneth E. Johnston, Randal N. Liu, Gui-Rong Liu, Shu-Lin |
author_sort | Tang, Le |
collection | PubMed |
description | BACKGROUND: The bacterial genus Salmonella contains thousands of serotypes that infect humans or other hosts, causing mild gastroenteritis to potentially fatal systemic infections in humans. Pathogenically distinct Salmonella serotypes have been classified as individual species or as serological variants of merely one or two species, causing considerable confusion in both research and clinical settings. This situation reflects a long unanswered question regarding whether the Salmonella serotypes exist as discrete genetic clusters (natural species) of organisms or as phenotypic (e.g. pathogenic) variants of a single (or two) natural species with a continuous spectrum of genetic divergence among them. Our recent work, based on genomic sequence divergence analysis, has demonstrated that genetic boundaries exist among Salmonella serotypes, circumscribing them into clear-cut genetic clusters of bacteria. METHODOLOGIES/PRINCIPAL FINDINGS: To further test the genetic boundary concept for delineating Salmonella into clearly defined natural lineages (e.g., species), we sampled a small subset of conserved genomic DNA sequences, i.e., the endonuclease cleavage sites that contain the highly conserved CTAG sequence such as TCTAGA for XbaI. We found that the CTAG-containing cleavage sequence profiles could be used to resolve the genetic boundaries as reliably and efficiently as whole genome sequence comparisons but with enormously reduced requirements for time and resources. CONCLUSIONS: Profiling of CTAG sequence subsets reflects genetic boundaries among Salmonella lineages and can delineate these bacteria into discrete natural clusters. |
format | Online Article Text |
id | pubmed-4138082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41380822014-08-20 CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters Tang, Le Liu, Wei-Qiao Fang, Xin Sun, Qiang Zhu, Song-Ling Wang, Chun-Xiao Wang, Xiao-Yu Li, Yong-Guo Zhu, Da-Ling Sanderson, Kenneth E. Johnston, Randal N. Liu, Gui-Rong Liu, Shu-Lin PLoS One Research Article BACKGROUND: The bacterial genus Salmonella contains thousands of serotypes that infect humans or other hosts, causing mild gastroenteritis to potentially fatal systemic infections in humans. Pathogenically distinct Salmonella serotypes have been classified as individual species or as serological variants of merely one or two species, causing considerable confusion in both research and clinical settings. This situation reflects a long unanswered question regarding whether the Salmonella serotypes exist as discrete genetic clusters (natural species) of organisms or as phenotypic (e.g. pathogenic) variants of a single (or two) natural species with a continuous spectrum of genetic divergence among them. Our recent work, based on genomic sequence divergence analysis, has demonstrated that genetic boundaries exist among Salmonella serotypes, circumscribing them into clear-cut genetic clusters of bacteria. METHODOLOGIES/PRINCIPAL FINDINGS: To further test the genetic boundary concept for delineating Salmonella into clearly defined natural lineages (e.g., species), we sampled a small subset of conserved genomic DNA sequences, i.e., the endonuclease cleavage sites that contain the highly conserved CTAG sequence such as TCTAGA for XbaI. We found that the CTAG-containing cleavage sequence profiles could be used to resolve the genetic boundaries as reliably and efficiently as whole genome sequence comparisons but with enormously reduced requirements for time and resources. CONCLUSIONS: Profiling of CTAG sequence subsets reflects genetic boundaries among Salmonella lineages and can delineate these bacteria into discrete natural clusters. Public Library of Science 2014-08-19 /pmc/articles/PMC4138082/ /pubmed/25137186 http://dx.doi.org/10.1371/journal.pone.0103388 Text en © 2014 Tang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tang, Le Liu, Wei-Qiao Fang, Xin Sun, Qiang Zhu, Song-Ling Wang, Chun-Xiao Wang, Xiao-Yu Li, Yong-Guo Zhu, Da-Ling Sanderson, Kenneth E. Johnston, Randal N. Liu, Gui-Rong Liu, Shu-Lin CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters |
title | CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters |
title_full | CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters |
title_fullStr | CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters |
title_full_unstemmed | CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters |
title_short | CTAG-Containing Cleavage Site Profiling to Delineate Salmonella into Natural Clusters |
title_sort | ctag-containing cleavage site profiling to delineate salmonella into natural clusters |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4138082/ https://www.ncbi.nlm.nih.gov/pubmed/25137186 http://dx.doi.org/10.1371/journal.pone.0103388 |
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