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The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life
The Snail transcription factor regulates diverse aspects of stem cell biology in organisms ranging from Drosophila to mammals. Here we have asked whether it regulates the biology of neural precursor cells (NPCs) in the forebrain of postnatal and adult mice, taking advantage of a mouse containing a f...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4138084/ https://www.ncbi.nlm.nih.gov/pubmed/25136812 http://dx.doi.org/10.1371/journal.pone.0104767 |
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author | Zander, Mark A. Cancino, Gonzalo I. Gridley, Thomas Kaplan, David R. Miller, Freda D. |
author_facet | Zander, Mark A. Cancino, Gonzalo I. Gridley, Thomas Kaplan, David R. Miller, Freda D. |
author_sort | Zander, Mark A. |
collection | PubMed |
description | The Snail transcription factor regulates diverse aspects of stem cell biology in organisms ranging from Drosophila to mammals. Here we have asked whether it regulates the biology of neural precursor cells (NPCs) in the forebrain of postnatal and adult mice, taking advantage of a mouse containing a floxed Snail allele (Snail(fl/fl) mice). We show that when Snail is inducibly ablated in the embryonic cortex, this has long-term consequences for cortical organization. In particular, when Snail(fl/fl) mice are crossed to Nestin-cre mice that express Cre recombinase in embryonic neural precursors, this causes inducible ablation of Snail expression throughout the postnatal cortex. This loss of Snail causes a decrease in proliferation of neonatal cortical neural precursors and mislocalization and misspecification of cortical neurons. Moreover, these precursor phenotypes persist into adulthood. Adult neural precursor cell proliferation is decreased in the forebrain subventricular zone and in the hippocampal dentate gyrus, and this is coincident with a decrease in the number of adult-born olfactory and hippocampal neurons. Thus, Snail is a key regulator of the numbers of neural precursors and newborn neurons throughout life. |
format | Online Article Text |
id | pubmed-4138084 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41380842014-08-20 The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life Zander, Mark A. Cancino, Gonzalo I. Gridley, Thomas Kaplan, David R. Miller, Freda D. PLoS One Research Article The Snail transcription factor regulates diverse aspects of stem cell biology in organisms ranging from Drosophila to mammals. Here we have asked whether it regulates the biology of neural precursor cells (NPCs) in the forebrain of postnatal and adult mice, taking advantage of a mouse containing a floxed Snail allele (Snail(fl/fl) mice). We show that when Snail is inducibly ablated in the embryonic cortex, this has long-term consequences for cortical organization. In particular, when Snail(fl/fl) mice are crossed to Nestin-cre mice that express Cre recombinase in embryonic neural precursors, this causes inducible ablation of Snail expression throughout the postnatal cortex. This loss of Snail causes a decrease in proliferation of neonatal cortical neural precursors and mislocalization and misspecification of cortical neurons. Moreover, these precursor phenotypes persist into adulthood. Adult neural precursor cell proliferation is decreased in the forebrain subventricular zone and in the hippocampal dentate gyrus, and this is coincident with a decrease in the number of adult-born olfactory and hippocampal neurons. Thus, Snail is a key regulator of the numbers of neural precursors and newborn neurons throughout life. Public Library of Science 2014-08-19 /pmc/articles/PMC4138084/ /pubmed/25136812 http://dx.doi.org/10.1371/journal.pone.0104767 Text en © 2014 Zander et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zander, Mark A. Cancino, Gonzalo I. Gridley, Thomas Kaplan, David R. Miller, Freda D. The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life |
title | The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life |
title_full | The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life |
title_fullStr | The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life |
title_full_unstemmed | The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life |
title_short | The Snail Transcription Factor Regulates the Numbers of Neural Precursor Cells and Newborn Neurons throughout Mammalian Life |
title_sort | snail transcription factor regulates the numbers of neural precursor cells and newborn neurons throughout mammalian life |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4138084/ https://www.ncbi.nlm.nih.gov/pubmed/25136812 http://dx.doi.org/10.1371/journal.pone.0104767 |
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