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Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran
BACKGROUND: Staphylococci release a large number of enzymes. Some of these, such as coagulase, beta- lactamase, hemolysins and biofilms are considered indices of pathogenicity. OBJECTIVES: The aim of the current study was based on the isolation and identification of Staphylococcus aureus and coagula...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4138617/ https://www.ncbi.nlm.nih.gov/pubmed/25147697 http://dx.doi.org/10.5812/jjm.9225 |
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author | Ebrahimi, Azizollah Ghasemi, Maryam Ghasemi, Bahram |
author_facet | Ebrahimi, Azizollah Ghasemi, Maryam Ghasemi, Bahram |
author_sort | Ebrahimi, Azizollah |
collection | PubMed |
description | BACKGROUND: Staphylococci release a large number of enzymes. Some of these, such as coagulase, beta- lactamase, hemolysins and biofilms are considered indices of pathogenicity. OBJECTIVES: The aim of the current study was based on the isolation and identification of Staphylococcus aureus and coagulase negative Staphylococci (CNS) strains from various skin lesions and examining their biofilms, beta- lactamase, hemolysins production and antibiotic resistance pattern. MATERIALS AND METHODS: Sixty one infected wounds and 39 skin infections samples were collected and examined. After the culture and identification, examination for production of hemolysins, beta- lactamase, biofilm and susceptibility toward 9 antimicrobials was performed. RESULTS: Out of 75 isolated Staphylococci, sixty (80%) were biofilm producers. Two overall prevalence of 28.5% and 100% of ß-lactamase production were recorded for isolated S. aureus and CNS, respectively. Twenty out of 49 (40.8%), the same number of α- and β- hemolytic S. aureus, were isolated while six (12.24%) were ∂ -hemolysin producers. Twenty two of Twenty six (84.6%) isolates of CNS, were hemolysin producers that all were ∂ type. The S. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics, this sensitivity pattern for isolates from skin dermatitis is relatively low, though. CONCLUSIONS: High percentage of hemolysins, biofilm and beta lactamase production by isolated Staphylococci, suggests an important role of these virulence factors in the pathogenesis of isolated Staphylococci from dermatitis lesions. The S. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics. Only ciprofloxacin was found to be active against all isolates from dedermatitis lesions. |
format | Online Article Text |
id | pubmed-4138617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-41386172014-08-21 Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran Ebrahimi, Azizollah Ghasemi, Maryam Ghasemi, Bahram Jundishapur J Microbiol Research Article BACKGROUND: Staphylococci release a large number of enzymes. Some of these, such as coagulase, beta- lactamase, hemolysins and biofilms are considered indices of pathogenicity. OBJECTIVES: The aim of the current study was based on the isolation and identification of Staphylococcus aureus and coagulase negative Staphylococci (CNS) strains from various skin lesions and examining their biofilms, beta- lactamase, hemolysins production and antibiotic resistance pattern. MATERIALS AND METHODS: Sixty one infected wounds and 39 skin infections samples were collected and examined. After the culture and identification, examination for production of hemolysins, beta- lactamase, biofilm and susceptibility toward 9 antimicrobials was performed. RESULTS: Out of 75 isolated Staphylococci, sixty (80%) were biofilm producers. Two overall prevalence of 28.5% and 100% of ß-lactamase production were recorded for isolated S. aureus and CNS, respectively. Twenty out of 49 (40.8%), the same number of α- and β- hemolytic S. aureus, were isolated while six (12.24%) were ∂ -hemolysin producers. Twenty two of Twenty six (84.6%) isolates of CNS, were hemolysin producers that all were ∂ type. The S. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics, this sensitivity pattern for isolates from skin dermatitis is relatively low, though. CONCLUSIONS: High percentage of hemolysins, biofilm and beta lactamase production by isolated Staphylococci, suggests an important role of these virulence factors in the pathogenesis of isolated Staphylococci from dermatitis lesions. The S. aureus isolates from wound infections, show a high sensitivity pattern to all examined antibiotics. Only ciprofloxacin was found to be active against all isolates from dedermatitis lesions. Kowsar 2014-04-01 2014-04 /pmc/articles/PMC4138617/ /pubmed/25147697 http://dx.doi.org/10.5812/jjm.9225 Text en Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar Corp. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ebrahimi, Azizollah Ghasemi, Maryam Ghasemi, Bahram Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran |
title | Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran |
title_full | Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran |
title_fullStr | Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran |
title_full_unstemmed | Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran |
title_short | Some Virulence Factors of Staphylococci Isolated From Wound and Skin Infections in Shahrekord, IR Iran |
title_sort | some virulence factors of staphylococci isolated from wound and skin infections in shahrekord, ir iran |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4138617/ https://www.ncbi.nlm.nih.gov/pubmed/25147697 http://dx.doi.org/10.5812/jjm.9225 |
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