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Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome

Mayer-Rokitansky-Küster-Hauser (MRKH) is a rare syndrome characterized by congenital aplasia of the uterus and vagina. The most common procedure used for surgical reconstruction of the neovagina is the McIndoe vaginoplasty, which consists in creation of a vaginal canal covered with a full-thickness...

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Autores principales: Nodale, Cristina, Vescarelli, Enrica, D'Amici, Sirio, Maffucci, Diana, Ceccarelli, Simona, Monti, Marco, Benedetti Panici, Pierluigi, Romano, Ferdinando, Angeloni, Antonio, Marchese, Cinzia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139028/
https://www.ncbi.nlm.nih.gov/pubmed/25162002
http://dx.doi.org/10.1155/2014/201518
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author Nodale, Cristina
Vescarelli, Enrica
D'Amici, Sirio
Maffucci, Diana
Ceccarelli, Simona
Monti, Marco
Benedetti Panici, Pierluigi
Romano, Ferdinando
Angeloni, Antonio
Marchese, Cinzia
author_facet Nodale, Cristina
Vescarelli, Enrica
D'Amici, Sirio
Maffucci, Diana
Ceccarelli, Simona
Monti, Marco
Benedetti Panici, Pierluigi
Romano, Ferdinando
Angeloni, Antonio
Marchese, Cinzia
author_sort Nodale, Cristina
collection PubMed
description Mayer-Rokitansky-Küster-Hauser (MRKH) is a rare syndrome characterized by congenital aplasia of the uterus and vagina. The most common procedure used for surgical reconstruction of the neovagina is the McIndoe vaginoplasty, which consists in creation of a vaginal canal covered with a full-thickness skin graft. Here we characterized the autologous in vitro cultured vaginal tissue proposed as alternative material in our developed modified McIndoe vaginoplasty in order to underlie its importance in autologous total vaginal replacement. To this aim human vaginal mucosa cells (HVMs) were isolated from vaginal mucosa of patients affected by MRKH syndrome and characterized with respect to growth kinetics, morphology, PAS staining, and expression of specific epithelial markers by immunofluorescence, Western blot, and qRT-PCR analyses. The presence of specific epithelial markers along with the morphology and the presence of mucified cells demonstrated the epithelial nature of HMVs, important for an efficient epithelialization of the neovagina walls and for creating a functional vaginal cavity. Moreover, these cells presented characteristics of effective proliferation as demonstrated by growth kinetics assay. Therefore, the autologous in vitro cultured vaginal tissue might represent a highly promising and valid material for McIndoe vaginoplasty.
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spelling pubmed-41390282014-08-26 Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome Nodale, Cristina Vescarelli, Enrica D'Amici, Sirio Maffucci, Diana Ceccarelli, Simona Monti, Marco Benedetti Panici, Pierluigi Romano, Ferdinando Angeloni, Antonio Marchese, Cinzia Biomed Res Int Research Article Mayer-Rokitansky-Küster-Hauser (MRKH) is a rare syndrome characterized by congenital aplasia of the uterus and vagina. The most common procedure used for surgical reconstruction of the neovagina is the McIndoe vaginoplasty, which consists in creation of a vaginal canal covered with a full-thickness skin graft. Here we characterized the autologous in vitro cultured vaginal tissue proposed as alternative material in our developed modified McIndoe vaginoplasty in order to underlie its importance in autologous total vaginal replacement. To this aim human vaginal mucosa cells (HVMs) were isolated from vaginal mucosa of patients affected by MRKH syndrome and characterized with respect to growth kinetics, morphology, PAS staining, and expression of specific epithelial markers by immunofluorescence, Western blot, and qRT-PCR analyses. The presence of specific epithelial markers along with the morphology and the presence of mucified cells demonstrated the epithelial nature of HMVs, important for an efficient epithelialization of the neovagina walls and for creating a functional vaginal cavity. Moreover, these cells presented characteristics of effective proliferation as demonstrated by growth kinetics assay. Therefore, the autologous in vitro cultured vaginal tissue might represent a highly promising and valid material for McIndoe vaginoplasty. Hindawi Publishing Corporation 2014 2014-08-05 /pmc/articles/PMC4139028/ /pubmed/25162002 http://dx.doi.org/10.1155/2014/201518 Text en Copyright © 2014 Cristina Nodale et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nodale, Cristina
Vescarelli, Enrica
D'Amici, Sirio
Maffucci, Diana
Ceccarelli, Simona
Monti, Marco
Benedetti Panici, Pierluigi
Romano, Ferdinando
Angeloni, Antonio
Marchese, Cinzia
Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome
title Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome
title_full Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome
title_fullStr Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome
title_full_unstemmed Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome
title_short Characterization of Human Vaginal Mucosa Cells for Autologous In Vitro Cultured Vaginal Tissue Transplantation in Patients with MRKH Syndrome
title_sort characterization of human vaginal mucosa cells for autologous in vitro cultured vaginal tissue transplantation in patients with mrkh syndrome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139028/
https://www.ncbi.nlm.nih.gov/pubmed/25162002
http://dx.doi.org/10.1155/2014/201518
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