One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System

Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in...

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Autores principales: FUJII, Wataru, ONUMA, Asuka, SUGIURA, Koji, NAITO, Kunihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2014
Materias:
Technology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139508/
https://www.ncbi.nlm.nih.gov/pubmed/25110137
http://dx.doi.org/10.1262/jrd.2013-139
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author FUJII, Wataru
ONUMA, Asuka
SUGIURA, Koji
NAITO, Kunihiko
author_facet FUJII, Wataru
ONUMA, Asuka
SUGIURA, Koji
NAITO, Kunihiko
author_sort FUJII, Wataru
collection PubMed
description Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in all-alleles. F0 mice showed knockout phenotypes or no protein expression of target genes simultaneously, and these mutations were normally inherited in the next generation.
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spelling pubmed-41395082014-08-21 One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System FUJII, Wataru ONUMA, Asuka SUGIURA, Koji NAITO, Kunihiko J Reprod Dev Technology Report Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in all-alleles. F0 mice showed knockout phenotypes or no protein expression of target genes simultaneously, and these mutations were normally inherited in the next generation. The Society for Reproduction and Development 2014-05-04 2014-08 /pmc/articles/PMC4139508/ /pubmed/25110137 http://dx.doi.org/10.1262/jrd.2013-139 Text en ©2014 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Technology Report
FUJII, Wataru
ONUMA, Asuka
SUGIURA, Koji
NAITO, Kunihiko
One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
title One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
title_full One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
title_fullStr One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
title_full_unstemmed One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
title_short One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System
title_sort one-step generation of phenotype-expressing triple-knockout mice with heritable mutated alleles by the crispr/cas9 system
topic Technology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139508/
https://www.ncbi.nlm.nih.gov/pubmed/25110137
http://dx.doi.org/10.1262/jrd.2013-139
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